2007 Volume 18 Issue 1

Traceless solid-phase synthesis of 3-substituted isoxazoles from polystyrene-supported vinyl selenide
Qin Xin , Shou Ri Sheng , Shu Ying Lin , Xiao Ling Liu , Xian Huang
2007, 18(1): 1-3  doi: 10.1016/j.cclet.2006.11.001
[Abstract](121) [FullText HTML] [PDF 209KB](0)
Abstract:
A novel facile procedure for traceless solid-phase synthesis of 3-substituted isoxazoles in good yields and with excellent purities using polymer-supported vinyl selenide has been developed.
Baeyer-Villiger oxidation of ketones with hydrogen peroxide catalyzed by Sn-aniline complex
Qing Hua Zhang , Shou Feng Wang , Zi Qiang Lei
2007, 18(1): 4-6  doi: 10.1016/j.cclet.2006.11.036
[Abstract](123) [FullText HTML] [PDF 198KB](0)
Abstract:
Sn-aniline complex was prepared by a simple procedure. Cyclic and acyclic ketones were oxidized into lactones or esters with very high selectivity and yield with 30% hydrogen peroxide in the presence of Sn-aniline complex.
Synthesis and antiangiogenic activities of 5-amino-1,3-dihydro-1,3-dioxo-2H-isoindole-2-propanoic acid derivatives
Yong Deng , Da Cheng Yang , Yi Shen , Yu Guo Zhong
2007, 18(1): 7-9  doi: 10.1016/j.cclet.2006.11.031
[Abstract](121) [FullText HTML] [PDF 220KB](0)
Abstract:
Based on the structure-activity relationships and antiangiogenic mechanism of RGD-containing peptides, a series of 5-amino-1,3-dihydro-1,3-dioxo-2H-isoindole derivatives were synthesized. The structures were characterized by 1H NMR, MS and elementary analysis. There ability to inhibit angiogenesis were evaluated by chick embryo chorioallantoic membrane assay at 10-5 mol/L. Compounds 7a and 7b displayed obvious antiangiogenic activity.
Reduction of 4-nitrophenol catalyzed by nitroreductase
Jie Chen , Rong Ji Dai , Bin Tong , Sheng Yuan Xiao , Wei Wei Meng
2007, 18(1): 10-12  doi: 10.1016/j.cclet.2006.11.009
[Abstract](129) [FullText HTML] [PDF 330KB](0)
Abstract:
The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Aminophenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygeninsensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.
Cu(OAc)2 catalyzed Sonogashira cross-coupling reaction in amines
Sheng Mei Guo , Chen Liang Deng , Jin Heng Li
2007, 18(1): 13-16  doi: 10.1016/j.cclet.2006.11.019
[Abstract](122) [FullText HTML] [PDF 193KB](0)
Abstract:
A simple Cu(OAc)2 catalyzed Sonogashira coupling protocol is presented. It was found that the couplings of a variety of aryl halides with terminal alkynes were conducted smoothly to afford the corresponding desired products in moderate to excellent yields, using Cu(OAc)2 as the catalyst and Et3N as the solvent.
Photopromoted methoxycarbonylation of olefin with methyl formate by Co(OAc)2
Ying Na Cui , Jing Mei Yin , Da Bin Gao , Ying Ping Jia , Guang Yun Zhou , Shen Min Li
2007, 18(1): 17-20  doi: 10.1016/j.cclet.2006.11.021
[Abstract](140) [FullText HTML] [PDF 240KB](0)
Abstract:
The photopromoted methoxycarbonylation of olefin with methyl formate catalyzed by Co(OAc)2 at ambient conditions has been carried out. The results indicated that the reaction activity increased with the increasing temperature. Methyl formate decomposed into CO and CH3OH firstly under irradiation, and then the methoxycarbonylation of olefin proceeded under catalysis of Co(OAc)2.The mechanism of methyl formate participating in the methoxycarbonylation is verified by the IR analysis and the labeling experiments of CD3OD and CH318OH.
Preparation and characterization of amino or carboxyl-functionalized ionic liquids
Yue Qin Cai , Feng Lu , Yan Qing Peng , Gong Hua Song
2007, 18(1): 21-23  doi: 10.1016/j.cclet.2006.11.018
[Abstract](121) [FullText HTML] [PDF 234KB](0)
Abstract:
New functionalized ionic liquids, 1-carboxylmethyl-3-methylimimidazolium hexafluorophosphate or fluoborate and l-aminoethyl-3-methylimimidazolium hexafluorophosphate or fluoborate have been synthesized and investigated. The obtained amino or carboxyl-functionalized ionic liquids were all characterized by FT-IR, 1H NMR and MS (ESI) and their properties such as freezing point, viscosity, solubility, specific gravity, surface tension, and interfacial tension were also determined.
Preparation of fructone catalyzed by water-soluble Brønsted acid ionic liquids
Yuan Yuan Wang , Rong Wang , Liang Chun Wu , Li Yi Dai
2007, 18(1): 24-26  doi: 10.1016/j.cclet.2006.11.023
[Abstract](121) [FullText HTML] [PDF 175KB](0)
Abstract:
Fructone (2-methyl-2-ethylacetoacetate-1,3-dioxolane), a flavouring material, has been synthesized from ethyl acetoacetate and glycol using five water-soluble Brønsted acid ionic liquids as catalysts for the first time. The used Brønsted acid ionic liquids include[Hmim]Tfa,[Hmim]Tsa,[Hmim]BF4,[Bmim]HSO4,[Bmim]H2PO4, and[Hmim]BF4 showed the highest catalytic activity for the preparation of fructone. After reaction, the product could be isolated from the reaction system automatically, and the ionic liquid could be directly reused without dehydration.
Ionic liquid [bmim][BF4] acts as solvent and promoter for synthesis of halo-containing N-arylphthalimides
Dong Chu Chen , Hong Qi Ye , Hao Wu
2007, 18(1): 27-29  doi: 10.1016/j.cclet.2006.11.027
[Abstract](134) [FullText HTML] [PDF 222KB](0)
Abstract:
A new synthetic process of N-arylphthalimide and halo-containing N-arylphthalimides through the reaction between phthalic anhydride and aromatic amines bearing halo groups in [bmim] [BF4] was described, ionic liquid [bmim] [BF4] acted as the dual role of solvent and promoter.
Acidichromism in the LB film of bolaform Schiff base
Mei Fang Yin , Ti Feng Jiao , Ming Hua Liu
2007, 18(1): 30-32  doi: 10.1016/j.cclet.2006.11.008
[Abstract](122) [FullText HTML] [PDF 232KB](0)
Abstract:
A bolaform (BNC10) and single-headed (HNOA) amphiphilic Schiff bases containing naphthyl group were designed and their Langmuir-Blodgett films were investigated. It was found that both the LB films show acidichromism, i.e. a reversible color change upon alternatively exposing the films to HCl and NH3 gases, respectively. It was further found that the bolaform Schiff bases film could trap NH3 gas during the acidichromic process.
Convenient synthesis of β-allenic α-difluoromethylenephosphonic acid monoesters:Potential synthons for cyclic phosphate mimics
Yun Lin , Jin Tao Liu
2007, 18(1): 33-36  doi: 10.1016/j.cclet.2006.11.029
[Abstract](133) [FullText HTML] [PDF 227KB](0)
Abstract:
β-Allenic α-difluoromethylenephosphonic acid monoesters were prepared under mild conditions for the first time by hydrolyzing the corresponding diethyl phosphonates in aqueous sodium hydroxide solution.
Preparation of polymer-supported phosphine from ferrocene for palladium-catalyzed Suzuki-Miyaura cross-coupling reactions
Hong Wei Yu , Qing Song Tong , Yi Ru Peng , Li Jia , Ji Cheng Shi , Zi Lin Jin
2007, 18(1): 37-40  doi: 10.1016/j.cclet.2006.11.030
[Abstract](120) [FullText HTML] [PDF 250KB](0)
Abstract:
Polymer-supported phosphine ligand 3b derived from ferrocene was prepared, and applied in palladium-catalyzed Suzuki-Miyaura reactions. A range of aryl bromides can couple with phenylboronic acid to obtain corresponding biaryls in excellent yields.The recycling of the polymer-bound catalyst was tested without adding palladium.
Pharmacophore-guided design, synthesis and evaluation of quinazoline-arylpiperazines as new α1-adrenoceptor antagonists
Hao Fang , Min Yong Li , Lin Xia
2007, 18(1): 41-44  doi: 10.1016/j.cclet.2006.11.022
[Abstract](137) [FullText HTML] [PDF 574KB](0)
Abstract:
A series of arylpiperazinesquinazoline-2,4-diamine compounds were designed and synthesized based on pharmacophore for uro-selective α1-adrenoceptor antagonists and 3D chemical database searching. The in vitro functional analysis showed that compounds 9 and 14 showed better and similar α1-AR antagonistic activity compared with prazosin.
Design,synthesis and in vitro evaluation of a series thiazolidinediones analogs as PPAR modulators
Jun Feng , Ying Lu , Zhe Feng Cai , Shi Peng Zhang , Zong Ru Guo
2007, 18(1): 45-47  doi: 10.1016/j.cclet.2006.11.032
[Abstract](119) [FullText HTML] [PDF 225KB](0)
Abstract:
A series of thiazolidinediones analogs, as PPAR modulators, were designed, synthesized and evaluated in vitro.
Synthesis of a kind of geminal imidazolium ionic liquid with long aliphatic chains
Yun Sheng Ding , Min Zha , Jun Zhang , Seng Shan Wang
2007, 18(1): 48-50  doi: 10.1016/j.cclet.2006.11.037
[Abstract](124) [FullText HTML] [PDF 232KB](0)
Abstract:
A novel geminal imidazolium ionic liquid with long hydrocarbon group, 1,4-bis (3-tetradecylimidazolium-1-yl) butane bromide was synthesized and an efficient synthesized method was introduced detailedly. Its structure was determined by FT-IR, 1H NMR and elemental analysis.
Synthesis of water-soluble cystine C60 derivative with catalyst and its active oxygen radical scavenging ability
Zhen Hu , Wen Chao Guan , Xiang Ying Tang , Li Zhen Huang , Hong Xu
2007, 18(1): 51-54  doi: 10.1016/j.cclet.2006.11.013
[Abstract](121) [FullText HTML] [PDF 264KB](0)
Abstract:
A novel water-soluble cystine C60 derivative was synthesized in the presence of the catalyst, tetrabutylammonium hydroxide (TBAH). The product was characterized by FT-IR, UV, 1H NMR, 13C NMR, MS and elemental analysis. Furthermore, the scavenging ability to superoxygen anion radical O2·- and hydroxyl radical ·OH was studied by chemiluminescence.It was found that cystine C60 derivative showed an excellent efficiency in eliminating superoxygen anion radical and hydroxyl radical. The 50%inhibition concentration (IC50) for superoxygen anion radical and hydroxyl radical were 0.167 and 0.008 mg/mL, respectively.
A novel plant defensin from Chinese mistletoe,Viscum coloratum (Kom.) Nakai
Shi Lei Liu , Xiu Bao Du , Jing Lin Kong , Hui Jiang
2007, 18(1): 55-58  doi: 10.1016/j.cclet.2006.11.007
[Abstract](121) [FullText HTML] [PDF 728KB](0)
Abstract:
A novel polypeptide named as defensin CM was isolated from Chinese mistletoe, Viscum coloratum (Kom.) Nakai. The amino acid sequence was determined by the combination of Edman degradation, endoproteinase Lys-C digestion, and MALDI-TOF mass spectrometry. The primary structure was ATCSAPSGRF KGACFSSNTC SNICKTLEGL KDGHCTGLAC YCSRNC.
Four new eremophilendiolides from Ligularia atroviolacea
Shu Yun Shi , Di Yao Wu , Xun Gong , Hua Peng , Rong Ping Zhang , Chang Xin Zhou , Xiao Jiang Hao , Zhong Jian Jia , Joachim StÖckigt , Yu Zhao
2007, 18(1): 59-61  doi: 10.1016/j.cclet.2006.11.004
[Abstract](121) [FullText HTML] [PDF 241KB](0)
Abstract:
From Ligularia atroviolacea, four new eremophilendiolides, 8β-hydroxy-eremophil-3,7 (11)-dien-12,8α(14,6α)-diolide (1),8β-methoxy-eremophil-3,7(11)-dien-12,8α(14,6α)-diolide (2), 8α-hydroxy-eremophil-3,7(11)-dien-12,8β(14,6α)-diolide (3) and eremophil-3,7(11),8-trien-12,8 (14,6α)-diolide (4), as well as a known diolide (5) were isolated. Their structures were elucidated on the basis of 1D and 2D NMR as well as ESI-MS spectral data.
A new triterpene and a saponin from Centella asiatica
Quan Lin Yu , Hong Quan Duan , Wen Yuan Gao , Yoshihisa Takaishi
2007, 18(1): 62-64  doi: 10.1016/j.cclet.2006.11.033
[Abstract](129) [FullText HTML] [PDF 203KB](0)
Abstract:
A new triterpene and a saponin, named 2α,3β,23-trihydroxyurs-20-en-28-oic acid (1) and 2α,3β,23-trihydroxyurs-20-en-28-oic acid O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl ester (2), have been isolated from the aerial part of Centella asiatica. Their structures were elucidated by spectral methods, including 2D-NMR spectra.
Diastereoisomeric saponins from the rhizomes of Tupistra chinensis
Kun Zou , Jun Wu , Ming Du , Chuang Liu , Zi Chun Tang , Jun Zhi Wang
2007, 18(1): 65-68  doi: 10.1016/j.cclet.2006.11.024
[Abstract](120) [FullText HTML] [PDF 222KB](0)
Abstract:
A pair of diastereoisomeric furostanol saponins was obtained from the n-butanol fraction of methanol extract from Tupistra chinensis rhizomes, a folk medicine of Shennongjia Forest District of Hubei Province. Their structures were determined, on the basis of chemical and spectroscopic evidences.
A new resveratrol tetramer from Caragana rosea
Guo Xun Yang , Chang Qi Hu
2007, 18(1): 69-72  doi: 10.1016/j.cclet.2006.11.017
[Abstract](126) [FullText HTML] [PDF 289KB](0)
Abstract:
In a continual effort to search for any anti-HIV agent from traditional Chinese medicine, one new resveratrol tetramer,cararosinols B, was isolated from the ethanol extract of aerial parts of Caragana rosea. Its structure was elucidated by spectroscopic analysis and comparison with known compounds.
A new eudesmane sesquiterpene glycoside from Parepigynum funingense
Yan Hua , Jun Zhou , Chang Xiang Chen
2007, 18(1): 73-75  doi: 10.1016/j.cclet.2006.11.026
[Abstract](120) [FullText HTML] [PDF 211KB](0)
Abstract:
A new eudesmane sesquiterpene glycoside, 1α,6β-dihydroxy-5,10-bis-epi-eudesm-4(15)-ene-6-O-β-D-glucopyranoside (2),together with a known analogue compound, 1α,6β-dihydroxy-5,10-bis-epi-eudesm-3-ene-6-O-β-D-glucopyranoside (1), were isolated from the roots of Parepigynum funingense. The structure of 2 was determined by 1D and 2D NMR spectroscopy. Compound 1 was isolated from this plant for the first time.
Emitting stability of poly(9,9-dialkylfluorene-co-N-butylcarbazole) by solid-state oxidative coupling polymerization
Wei Bin Bai , Zhi Yuan Chen , Cai Mao Zhan
2007, 18(1): 76-78  doi: 10.1016/j.cclet.2006.11.002
[Abstract](126) [FullText HTML] [PDF 194KB](0)
Abstract:
Dihexylfluorene and N-butylcarbazole were copolymerized by solid-state oxidative coupling polymerization in the presence of anhydrous FeCl3 at room temperature. The solid-state films of the copolymers emitted blue light after heating at 150℃ in air for 24 h, no red-shifted emission was observed by fluorescence spectroscopy.
Studies on LCST of poly (N-isopropylacrylamide-co-acrylic acid-co-N-diacetone acrylamide)
Cai Hua Ni , Xiao Xia Zhu , Qing Lan Wang , Xian Yu Zeng
2007, 18(1): 79-80  doi: 10.1016/j.cclet.2006.11.003
[Abstract](125) [FullText HTML] [PDF 211KB](0)
Abstract:
Linear copolymers from N-isopropylacrylamide (NIPA), acrylic acid (AA) and diacetone acrylamide (DAA) have been prepared.The effect of composition, ionic strength and pH on their lower critical solution temperature (LCST) has been investigated.
A new chain-like heteropolytungstate formed by Keggin cluster units:Synthesis and structure of [H2bpy]3 [SiMnW11O39]·1.25H2O
Jing Xin Meng , Ying Ma , Xin Xin Xu , Yang Guang Li , En Bo Wang
2007, 18(1): 81-84  doi: 10.1016/j.cclet.2006.11.020
[Abstract](131) [FullText HTML] [PDF 2067KB](0)
Abstract:
A new heteropolytungstate,[H2bpy]3[SiMnW11O39]·1.25H2O (1), has been prepared under mild hydrothermal conditions and structurally characterized by single crystal X-ray diffraction. It is the first characterized compound containing 1D zigzag chain of transition metal substituted Keggin heteropolytungstate, which is connected through a common oxygen atom. The elemental analysis and IR of it are also described.
Synthesis and antibacterial activity of new layered perovskite compounds, AgxNa2-xLa2Ti3O10
Shao Zao Tan , Liu Cai Ding , Ying Liang Liu , You Sheng Ouyang , Yi Ben Chen
2007, 18(1): 85-88  doi: 10.1016/j.cclet.2006.11.025
[Abstract](123) [FullText HTML] [PDF 229KB](0)
Abstract:
Three kinds of new layered perovskite compounds with Ruddlesden-Popper (R-P) phase, AgxNa2-xLa2Ti3O10 (x=0.2, 0.3 and 0.5), were synthesized by an ion-exchange reaction of Na2La2Ti3O10 with AgNO3 solution. The structures of the compounds were characterized by EDX and XRD, and their antibacterial activity and light-resistance property were evaluated. The results indicated that the molecular formula of AgxNa2-xLa2Ti3O10 (x=0.2, 0.3 and 0.5) was confirmed, and that the crystalline structure of Na2La2Ti3O10 was not obviously affected by exchange of silver ion. The minimum inhibitory concentrations (MICs) of Ag0.3Na1.7La2Ti3O10 against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) were 180 μg/mL and 240 μg/mL,respectively, while its discoloration was not observed after 24 h light ageing test.
Fluorescence property of tris (2, 2'-bipyridine) ruthenium (Ⅱ) dichloride immobilized in the gallery of γ-zirconium phosphates
Ming Hui Xiang , Xian Bo Shi , Na Li , Ke An Li
2007, 18(1): 89-90  doi: 10.1016/j.cclet.2006.11.038
[Abstract](128) [FullText HTML] [PDF 217KB](0)
Abstract:
Tris (2, 2'-bipyridine) ruthenium (Ⅱ) (Ru(bpy)32+) dichloride was successfully immobilized into the galleria of layered gamma zirconium phosphate (γ-ZrP) preintercalated by butyl amine(BA). The compounds showed extended luminescence lifetime of about four times of unimmobilized metal complex.
Capillary electrophoresis-chemiluminecence detection of levodopa and benserazide in Medopar tablet
Wei Wei He , Xing Wang Zhou , Jian Quan Lu
2007, 18(1): 91-93  doi: 10.1016/j.cclet.2006.11.034
[Abstract](123) [FullText HTML] [PDF 170KB](0)
Abstract:
A novel method for the simultaneous determination of benserazide and levodopa using capillary electrophoresis (CE)-chemiluminescence (CL) has been developed. Under the optimal conditions, the detection limits (S/N=3) were 1.85 μg/mL for benserazide and 0.12 μg/mL for levodopa. This method was successfully applied to the determination of benserazide and levodopa in Medopar tablet, the results showed that the detected values are in accordance with those by official methods.
Voltammetric studies on the interaction of amikacin with methyl blue and its analytical application
Xue Liang Wang , Zhang Yu Yu , Kui Jiao
2007, 18(1): 94-96  doi: 10.1016/j.cclet.2006.11.028
[Abstract](130) [FullText HTML] [PDF 214KB](0)
Abstract:
A new method to determine the concentration of amikacin (AMK) using methyl blue (MB) as electrochemical probe was developed in this paper. In pH 4.5 Britton-Robinson (B-R) buffer solution, the MB reacted with AMK to form ion association complexes, which led to the reductive peak current of MB at -0.275 V (versus SCE) to decrease, and the decreases were linear with the concentration of AMK in the range of 1.0-60.0 mg/L, the regression of equation is AIp (nA)=-8.48+102.36c (mg/L),correlation coefficient γ is 0.997. The conditions for determining the concentration of AMK using linear sweep voltammetry (SLV) were optimized. The method was used to determine the content of amikacin commercially available with satisfactory results.
Study of the interaction of Zn2+ with Aβ(10-21) by fluorescamine assay
Chao Feng Zhang , Li Fan , Pin Yang
2007, 18(1): 97-98  doi: 10.1016/j.cclet.2006.11.016
[Abstract](127) [FullText HTML] [PDF 238KB](0)
Abstract:
Zinc may play a role as a co-factor in the pathogenesis of Alzheimer's disease (AD) through influencing the conformation and neurotoxicity of amyloid β-protein (Aβ). Using the fluorescamine assay, we show for the first time that Zn2+induced Aβ(10-21) aggregate in a concentration-dependent manner. These results indicate that Aβ(10-21) can be used as an in vitro model in Zn2+-induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ(1-42).
Sequential analysis of dimethyl sulfur compounds in seawater
Meng Li , Dong Xing Yuan , Quan Long Li , Xiao Ying Jin
2007, 18(1): 99-102  doi: 10.1016/j.cclet.2006.11.005
[Abstract](118) [FullText HTML] [PDF 312KB](0)
Abstract:
A sequential method for the determination of dimethyl sulfur compounds, including dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO), in seawater samples has been developed. Detection limit of 2.5 pmol of DMS in 25 mL sample, corresponding to 0.10 nmol/L, was achieved. Recoveries for dimethyl sulfur compounds were in the range of 68.6-78.3%. The relative standard deviations (R.S.D.s) for DMS, DMSP and DMSO determination were 3.0, 5.4 and 7.4%, respectively.
Dioxygen affinities and catalytic oxidation performance of cobalt(Ⅱ) hydroxamates with benzo-15-crown-5 pendants
Hong Bo Li , Chuan Qin , Wen Bing Yang , Xiao Ping Hu , Sheng Ying Qin
2007, 18(1): 103-106  doi: 10.1016/j.cclet.2006.11.011
[Abstract](131) [FullText HTML] [PDF 255KB](0)
Abstract:
The oxygenation constants (Ko2) of cobalt(Ⅱ) hydroxamates (CoL21-CoL23) with benzo-15-crown-5 (B15C5) pendants were measured over the range of -5 to +20℃, and the values of thermodynamic parameters (△H0 and △S0) were calculated based on these (Ko2) values. Meanwhile, these crowned complexes were employed to the oxidation for p-xylene to p-toluic acid with air at 110△ under normal atmospheric pressure. The effects of B15C5 pendant and the length of chain bonded to B15C5 in these complexes on the O2-binding capabilities and oxidafon for p-xylene were investigated with the comparison of crown-free analogues CoL24.
Rh(Ⅱ) carbene S-H insertion into H2S via the stepwise mechanism
Zhen Feng Liu , Xian Fang Yue , Qiang Wei , Ke Li Han
2007, 18(1): 107-110  doi: 10.1016/j.cclet.2006.11.012
[Abstract](128) [FullText HTML] [PDF 1193KB](0)
Abstract:
The mechanism of Rh(Ⅱ) carbene S-H insertion into H2S in gas phase has been studied by B3LYP functional. Calculation results showed that the Rh(Ⅱ) carbene S-H insertion into H2S took only stepwise channels and no concerted channels had been located,which was different from the Rh(Ⅱ) carbene C-H and O-H insertions.
A novel strategy for in situ maskless synthesis of biochips:Self-driving micro-fluid porous type printing (SMPTP)
Nong Yue He , Ya Fei Guo , Song Li , Jian Xin Tang
2007, 18(1): 111-114  doi: 10.1016/j.cclet.2006.11.015
[Abstract](131) [FullText HTML] [PDF 877KB](0)
Abstract:
A novel maskless technique, self-driving micro-fluid porous type printing (SMPTP), was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple craft. In SMPTP for fabricating genechips, porous fiber tubes with a number of nanometric or micron channels functioned as "active letters" and were assembled in designed patterns, which are identical to the distribution of monomers in each layer of the array, and four patterns were needed for each layer. By means of capillarity, the synthesis solution was automatically taken into porous tubes assembled in a printing plate and reached the surface. An oligonucleotide array of 160 features with four different 15-mer probes was in situ synthesized using this technique. The four specific oligonucleotide probes, including the matched and the mismatched by the fluorescent target sequence, gave obviously different hybridization fluorescent signals.
Ion-ion reactions for charge reduction of biopolymer at atmospheric pressure ambient
Yue Ming Zhou , Jian Hua Ding , Xie Zhang , Huan Wen Chen
2007, 18(1): 115-117  doi: 10.1016/j.cclet.2006.11.010
[Abstract](130) [FullText HTML] [PDF 347KB](0)
Abstract:
Extractive electrospray ionization source (EESI) was adapted for ion-ion reaction, which was demonstrated by using a linear quadrupole ion trap mass spectrometer for the first ion-ion reaction of biopolymers in the atmospheric pressure ambient.
Preparation and characterization of crosslinked chitosan-based nanofibers
Ying Shan Zhou , Dong Zhi Yang , Jun Nie
2007, 18(1): 118-120  doi: 10.1016/j.cclet.2006.11.035
[Abstract](129) [FullText HTML] [PDF 2390KB](0)
Abstract:
Crosslinked chitosan-based nanofibers were successfully prepared via electrospinning technique with heat mediated chemical crosslinking followed. The structure, morphology and mechanical property of nanofibers were characterized by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), Instron machine, respectively. The results showed that, nanofibers exhibited a smooth surface and regular morphology, and tensile strength of nanofibers improved with increasing of triethylene glycol dimethacrylate (TEGDMA) content.
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