Citation:
Jie Chen, Rong Ji Dai, Bin Tong, Sheng Yuan Xiao, Wei Wei Meng. Reduction of 4-nitrophenol catalyzed by nitroreductase[J]. Chinese Chemical Letters,
;2007, 18(1): 10-12.
doi:
10.1016/j.cclet.2006.11.009
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The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Aminophenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygeninsensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.
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Keywords:
- Nitroreductase,
- 4-Nitrophenol,
- Biotransformation,
- Reduction
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