Citation: Xiaoyu Song, Xinghua Liu, Lei Xu, Aochi Liu, Xiangyu Meng, Yuening Wang, Mingjian Zhang, Guangcheng Xi, Jie Lin, Xiaotian Wang, Gengchen Xie. Sub-nanoscale oxygen-defect-rich MoO3-x: A versatile platform for label-free ultrasensitive SERS biodetection[J]. Chinese Journal of Structural Chemistry, ;2026, 45(4): 100848. doi: 10.1016/j.cjsc.2025.100848 shu

Sub-nanoscale oxygen-defect-rich MoO3-x: A versatile platform for label-free ultrasensitive SERS biodetection

Although surface-enhanced Raman scattering (SERS) technology has been widely applied in fields such as environmental pollutant monitoring and early cancer diagnosis, the design of universal substrates to enable high-sensitivity detection across multiple scenarios remains a pressing problem to be solved. In this study, an innovative defect engineering strategy was employed to successfully develop a SERS substrate based on molybdenum oxide sub-nanowires (MoO3-x Sub-NWs) with a high oxygen vacancy (Vo) concentration. This substrate uniquely combines the gradient defect states induced by Vo and the quantum confinement effect generated by the one-dimensional sub-nanostructure, thereby achieving the synergy of chemical enhancement and electromagnetic enhancement. Experimental results demonstrate that the substrate exhibits an enhancement factor as high as 7.8×107 for rhodamine 6G, achieves a LOD of 10-11 M for dyes such as methyl orange, and enables effective detection of various environmental pollutants including polychlorinated phenols, polycyclic aromatic hydrocarbons, and polystyrene microspheres. In terms of biomedical applications, based on the PCA-LDA model, the method achieves a three-category classification accuracy of 92.22% for hepatocellular carcinoma cells (HepG2), esophageal cancer cells (TE-1), and white blood cells (WBC), a discrimination accuracy of 90% for esophageal cancer subtypes (TE-1 and KYSE), and an ROC curve AUC value of 0.97. This study provides a new paradigm for the development of high-performance universal SERS substrates and possesses significant application value in the fields of environmental monitoring and non-invasive tumor diagnosis.
- MoO3-x Sub-NWs,
- Oxygen vacancy engineering,
- SERS,
- Environmental monitoring & cancer subtyping
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沈阳化工大学材料科学与工程学院沈阳 110142