Citation: Ling Sun, Xiaoqing Wei, Yingping Li, Junzhen Deng, Jingya Zhao, Shaobing Zhou. Micro/nano technology-driven diagnostic platforms: Addressing the speed dilemma in antimicrobial susceptibility testing[J]. Chinese Chemical Letters, ;2026, 37(12): 112737. doi: 10.1016/j.cclet.2026.112737 shu

Micro/nano technology-driven diagnostic platforms: Addressing the speed dilemma in antimicrobial susceptibility testing

Ling Sun ^{a, b} ,
Xiaoqing Wei ^a ,
Yingping Li ^{a, b} ,
Junzhen Deng ^a ,
Jingya Zhao ^{a, *,} ,
Shaobing Zhou ^a

a.
Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China
b.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China

jyzhao@swjtu.edu.cn

Received Date: 17 July 2025
Revised Date: 2 April 2026
Accepted Date: 6 April 2026
Available Online: 7 April 2026

Figures(8)

The inappropriate use of antibiotics has accelerated antimicrobial resistance development. Antimicrobial susceptibility testing (AST) is a critical tool for guiding clinical antibiotic administration and mitigating the progression of antimicrobial resistance. A major limitation of conventional AST methods is their prolonged duration, which severely restricts their clinical utility. Recent advances in micro/nano technology have offered innovative solutions to this longstanding bottleneck. By leveraging nanomaterials and micro/nano devices, novel AST platforms have been developed that dramatically reduce detection time. This review summarizes recent progress in micro/nano technology-based AST platforms, highlights the outstanding performance of nanomaterials and micro/nano devices in accelerating AST, and discusses future prospects for their clinical translation.
- Micro/nano technology,
- Antimicrobial susceptibility testing,
- Biosensor,
- Rapid detection,
- Antimicrobial resistance
1. [1]
  G.A.R. Collaborators, Lancet 404 (2024) 1199–1226. doi: 10.1016/S0140-6736(24)01867-1
2. [2]
  R.C. MacLean, A.S. Millan, Science 365 (2019) 1082–1083. doi: 10.1126/science.aax3879
3. [3]
  H. Zhao, L. Wei, H. Li, et al., Lancet Infect. Dis. 21 (2021) 847–857. doi: 10.1016/S1473-3099(20)30596-X
4. [4]
  D. Nathwani, D. Varghese, J. Stephens, et al., Antimicrob. Resist. Infect. Control. 8 (2019) 35. doi: 10.1186/s13756-019-0471-0
5. [5]
  F. Baquero, B.R. Levin, Nat. Rev. Microbiol. 19 (2021) 123–132. doi: 10.1038/s41579-020-00443-1
6. [6]
  A. Bolotsky, R. Muralidharan, D. Butler, et al., Biosens. Bioelectron. 172 (2021) 112615. doi: 10.1016/j.bios.2020.112615
7. [7]
  E. Ruiz-Hitzky, M. Darder, B. Wicklein, et al., Adv. Healthc. Mater. 9 (2020) e2000979. doi: 10.1002/adhm.202000979
8. [8]
  W. Gao, L. Zhang, Nat. Rev. Microbiol. 19 (2021) 5–6. doi: 10.1038/s41579-020-00469-5
9. [9]
  J. Shen, X. Zhou, Y. Shan, et al., Nat. Commun. 11 (2020) 267. doi: 10.1038/s41467-019-14135-9
10. [10]
  L. Li, M. Liu, S. Deng, et al., Small 19 (2023) e2208249. doi: 10.1002/smll.202208249
11. [11]
  M. Agar, M. Laabei, H.S. Leese, P. Estrela, Biosens. Bioelectron. 272 (2025) 117136. doi: 10.1016/j.bios.2025.117136
12. [12]
  X. Huang, L. Li, Z. Chen, et al., Adv. Mater. 35 (2023) e2302431. doi: 10.1002/adma.202302431
13. [13]
  Z. Ma, H. Wang, Z. Shi, et al., ACS Nano 17 (2023) 5740–5756. doi: 10.1021/acsnano.2c12165
14. [14]
  J. Wang, B. Hao, K. Xue, et al., Adv. Mater. 34 (2022) e2205653. doi: 10.1002/adma.202205653
15. [15]
  Y. Wang, F. Wu, Y. Li, et al., Mater. Horiz. 11 (2024) 4781–4790. doi: 10.1039/d4mh00642a
16. [16]
  L. Yu, Y. Sun, Y. Niu, et al., Adv. Healthc. Mater. 12 (2023) e2202596. doi: 10.1002/adhm.202202596
17. [17]
  J. Zeng, C. Gu, X. Geng, et al., Small 20 (2024) e2307096. doi: 10.1002/smll.202307096
18. [18]
  Z. Zhang, J. Xie, J. Xing, et al., Adv. Healthc. Mater. 12 (2023) e2201565. doi: 10.1002/adhm.202201565
19. [19]
  Z. Zhang, H. Yan, B. Qiu, et al., Small 18 (2022) e2200813. doi: 10.1002/smll.202200813
20. [20]
  W. Zhong, S. Handschuh-Wang, U.T. Uthappa, et al., ACS Nano 18 (2024) 32335–32363. doi: 10.1021/acsnano.4c11430
21. [21]
  K.P. Survanshu Saxena, Nadim Ahamad, Subhasini Singh, Prachi Bendale, Rinti Banerjee, ACS Biomater. Sci. Eng. 8 (2022) 2232–2257. doi: 10.1021/acsbiomaterials.1c01516
22. [22]
  Z. Liang, Z. Zeng, Y. Liao, et al., Front. Microbiol. 15 (2024) 1501204. doi: 10.3389/fmicb.2024.1501204
23. [23]
  Z. Zhang, Y. Wang, Y. Pang, C. Liu, Antimicrob. Agents Chemother. 58 (2014) 5632–5635. doi: 10.1128/AAC.02778-14
24. [24]
  T.P. Cusack, E.A. Ashley, C.L. Ling, et al., Clin. Microbiol. Infect. 25 (2019) 910–911. doi: 10.1016/j.cmi.2019.03.007
25. [25]
  A. Baraz, M. Chowers, D. Nevo, U. Obolski, Clin. Microbiol. Infect. 29 (2023) 390 e391-390 e394.
26. [26]
  G. Palladini, C. Garbarino, A. Luppi, et al., J. Microbiol. Methods 212 (2023) 106796. doi: 10.1016/j.mimet.2023.106796
27. [27]
  T.P. Lazou, S.C. Chaintoutis, J. Microbiol. Methods 204 (2023) 106649. doi: 10.1016/j.mimet.2022.106649
28. [28]
  G.M. Carvalho, B.A. Silva, R.G.C. Xavier, et al., Anaerobe 80 (2023) 102720. doi: 10.1016/j.anaerobe.2023.102720
29. [29]
  L.A. Arroyo, A. García-Curiel, M.E. Pachón-Ibañez, et al., J. Clin. Microbiol. 43 (2005) 903–905. doi: 10.1128/JCM.43.2.903-905.2005
30. [30]
  M. Vading, O. Samuelsen, B. Haldorsen, A.S. Sundsfjord, C.G. Giske, Clin. Microbiol. Infect. 17 (2011) 668–674. doi: 10.1111/j.1469-0691.2010.03299.x
31. [31]
  A. Garrido-Maestu, M. Prado, Compr. Rev. Food Sci. Food Saf. 21 (2022) 1913–1939. doi: 10.1111/1541-4337.12902
32. [32]
  F.R. Neuenschwander, B. Groß, S. Schubert, Antibiotics 12 (2023) 1042. doi: 10.3390/antibiotics12061042
33. [33]
  X. Lin, K. Zhai, B.M. Liu, J. Chen, Anal. Chem. 97 (2025) 17040–17049. doi: 10.1021/acs.analchem.5c02762
34. [34]
  K. Syal, R. Iriya, Y. Yang, et al., ACS Nano 10 (2015) 845–852.
35. [35]
  H.W. Cheng, H.M. Tsai, Y.L. Wang, Anal. Chem. 95 (2023) 16967–16975. doi: 10.1021/acs.analchem.3c03259
36. [36]
  M. Zhao, F. Cao, J. Chen, et al., Biosens. Bioelectron. 221 (2023) 114902. doi: 10.1016/j.bios.2022.114902
37. [37]
  S. Wei, Q. Tang, X. Hu, et al., ACS Sens. 9 (2024) 325–336. doi: 10.1021/acssensors.3c02014
38. [38]
  P. She, Y. Wang, Y. Liu, et al., Microbiologyopen 8 (2019) e933. doi: 10.1002/mbo3.933
39. [39]
  C. Chen, W. Hong, Antibiotics 10 (2021) 10030311.
40. [40]
  J. Gao, J. Guo, J. Chen, et al., Adv. Healthc. Mater. 11 (2022) e2101736. doi: 10.1002/adhm.202101736
41. [41]
  J. Gao, J. Qin, C. Ding, et al., RSC Chem. Biol. 3 (2022) 1314–1319. doi: 10.1039/d2cb00163b
42. [42]
  X. Gao, M. Li, M. Zhao, et al., Anal. Chem. 94 (2022) 6957–6966. doi: 10.1021/acs.analchem.1c05006
43. [43]
  J. Zhang, M. Wang, J. Xiao, et al., ACS Sens. 9 (2024) 379–387. doi: 10.1021/acssensors.3c02144
44. [44]
  R. Huang, X. Cai, J. Du, et al., ACS Nano 16 (2022) 19229–19239. doi: 10.1021/acsnano.2c08532
45. [45]
  Y. An, X. Fang, J. Cheng, et al., RSC Med. Chem. 15 (2024) 380–398. doi: 10.1039/d3md00581j
46. [46]
  J.H. Park, H.R. Kim, M.J. Kim, et al., ACS Appl. Mater. Interfaces 15 (2023) 56702–56717.
47. [47]
  X. Liu, X. Chen, S. Yin, et al., Chem. Eng. J. 499 (2024) 156506. doi: 10.1016/j.cej.2024.156506
48. [48]
  L. Sun, Y. Chen, Y. Duan, F. Ma, ACS Appl. Mater. Interfaces 13 (2021) 38923–38930. doi: 10.1021/acsami.1c11949
49. [49]
  L.J. Zhang, L. Xia, H.Y. Xie, Z.L. Zhang, D.W. Pang, Anal. Chem. 91 (2019) 532–547. doi: 10.1021/acs.analchem.8b04721
50. [50]
  A.M. Saeboe, A.Y. Nikiforov, R. Toufanian, et al., Nano Lett 21 (2021) 3271–3279. doi: 10.1021/acs.nanolett.1c00600
51. [51]
  L.L. Chen, L. Zhao, Z.G. Wang, S.L. Liu, D.W. Pang, Small 18 (2022) e2104567. doi: 10.1002/smll.202104567
52. [52]
  X. Jiang, H. Zeng, C. Duan, et al., Dalton Trans. 51 (2022) 3581–3589. doi: 10.1039/d1dt03624f
53. [53]
  C. Wang, B. Tang, J. Wu, et al., Int. J. Antimicrob. Agents 62 (2023) 106898. doi: 10.1016/j.ijantimicag.2023.106898
54. [54]
  A. Matsui, H. Niimi, Y. Uchiho, et al., Sci. Rep. 9 (2019) 13565. doi: 10.1038/s41598-019-49358-9
55. [55]
  J. Ki, I.H. Kwon, J. Lee, et al., J. Hazard. Mater. 460 (2023) 132398. doi: 10.1016/j.jhazmat.2023.132398
56. [56]
  D.R. Giacobbe, T. Giani, M. Bassetti, et al., Clin. Microbiol. Infect. 26 (2020) 713–722. doi: 10.1016/j.cmi.2019.09.023
57. [57]
  R. Aloni-Grinstein, O. Schuster, S. Yitzhaki, et al., Front. Microbiol. 8 (2017) 312.
58. [58]
  T.H. Kim, J. Kang, H. Jang, et al., Nature 632 (2024) 893–902. doi: 10.1038/s41586-024-07725-1
59. [59]
  M.P. Luyao Ma, Xiaonan Lu, Appl. Environ. Microb. 86 (2020) e00096-00020.
60. [60]
  A. Arienzo, L. Murgia, V. Cellitti, et al., PLoS One 18 (2023) e0284746. doi: 10.1371/journal.pone.0284746
61. [61]
  C.C. Chung, I.F. Cheng, H.M. Chen, et al., Anal. Chem. 84 (2012) 3347–3354. doi: 10.1021/ac300093w
62. [62]
  I.H. Su, W.C. Ko, C.H. Shih, et al., Anal Chem 89 (2017) 4635–4641. doi: 10.1021/acs.analchem.7b00220
63. [63]
  N. Jo, B. Kim, S.M. Lee, et al., Biosens. Bioelectron. 102 (2018) 164–170. doi: 10.1016/j.bios.2017.11.010
64. [64]
  H. Leonard, S. Halachmi, N. Ben-Dov, O. Nativ, E. Segal, ACS Nano 11 (2017) 6167–6177. doi: 10.1021/acsnano.7b02217
65. [65]
  F.E. Chen, A. Kaushik, K. Hsieh, et al., Anal. Chem. 93 (2021) 1260–1265. doi: 10.1021/acs.analchem.0c04095
66. [66]
  P.J.W. He, I.N. Katis, A.J.U. Kumar, et al., Biosens. Bioelectron. 152 (2020) 112008. doi: 10.1016/j.bios.2020.112008
67. [67]
  W.B. Lee, C.C. Chien, H.L. You, et al., Biosens. Bioelectron. 176 (2021) 112890. doi: 10.1016/j.bios.2020.112890
68. [68]
  C. Chen, Y. Wang, F. Wu, W. Hong, Anal. Chem. 95 (2023) 15556–15565. doi: 10.1021/acs.analchem.3c02243
69. [69]
  H. Li, K. Hsieh, P.K. Wong, et al., Nat. Rev. Methods Primers 3 (2023) 6. doi: 10.1038/s43586-022-00190-y
70. [70]
  P. Juskova, S. Schmitt, A. Kling, et al., ACS Sens 6 (2021) 2202–2210. doi: 10.1021/acssensors.1c00020
71. [71]
  G. Pitruzzello, S. Johnson, T.F. Krauss, Biosens. Bioelectron. 224 (2023) 115056. doi: 10.1016/j.bios.2022.115056
72. [72]
  V. Kandavalli, P. Karempudi, J. Larsson, J. Elf, Nat. Commun. 13 (2022) 6215. doi: 10.1038/s41467-022-33659-1
73. [73]
  P. Athamanolap, K. Hsieh, C.M. O’Keefe, et al., Anal. Chem. 91 (2019) 12784–12792. doi: 10.1021/acs.analchem.9b02344
74. [74]
  J. Riti, G. Sutra, T. Naas, et al., Biosens. Bioelectron. 257 (2024) 116301. doi: 10.1016/j.bios.2024.116301
75. [75]
  H.S. Cui, Z.R. Wu, X.Y. Shi, et al., ACS Sens 9 (2024) 3540–3548. doi: 10.1021/acssensors.4c00381
76. [76]
  R. Wang, S. Vemulapati, L.F. Westblade, et al., Anal. Chem. 92 (2020) 2731–2738. doi: 10.1021/acs.analchem.9b04991
77. [77]
  M.I. Villalba, P. Stupar, W. Chomicki, et al., Small 14 (2018) 1702671. doi: 10.1002/smll.201702671
78. [78]
  J. Zhou, C. Liao, M. Zou, et al., Nano Lett 24 (2024) 2980–2988. doi: 10.1021/acs.nanolett.3c03781
79. [79]
  A. Sturm, G. Jozwiak, M.P. Verge, et al., Nat. Commun. 15 (2024) 2037. doi: 10.1038/s41467-024-46213-y
80. [80]
  U. Theuretzbacher, S. Gottwalt, P. Beyer, et al., Lancet Infect. Dis. 19 (2019) e40-e50. doi: 10.1016/S1473-3099(18)30513-9
81. [81]
  E.M. Darby, E. Trampari, P. Siasat, et al., Nat. Rev. Microbiol. 21 (2023) 280–295. doi: 10.1038/s41579-022-00820-y
82. [82]
  Y.F. Tao, Y. Wang, S. Huang, et al., Anal. Chem. 89 (2017) 4108–4115. doi: 10.1021/acs.analchem.6b05051
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