Citation: Yang Song, Jin-Ming Lin. Microfluidic-based method for the separation and analysis of extracellular vesicles[J]. Chinese Chemical Letters, ;2026, 37(10): 112553. doi: 10.1016/j.cclet.2026.112553 shu

Microfluidic-based method for the separation and analysis of extracellular vesicles

Yang Song ,
Jin-Ming Lin ^*,

Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China

jmlin@mail.tsinghua.edu.cn

Received Date: 15 October 2025
Revised Date: 18 January 2026
Accepted Date: 25 February 2026
Available Online: 26 February 2026

Figures(12)

Extracellular vesicles (EVs) released by cells are widely present in body fluids and serve as mediators of cell communication and markers in disease diagnosis. However, it remains challenging to separate high-purity and structurally intact EVs from complex biological samples. Recently, microfluidics has shown excellent performance in the separation and analysis of EVs due to its precise control and integration capabilities. Microfluidic methods hold promise for clinical diagnostics owing to their integrated and convenient properties. In this review, we first introduce microfluidic-based label-free and affinity-based strategies for EV separation. Next, the common EV analysis methods integrated with microfluidics are summarized, including fluorescence, electrochemistry, surface plasmon resonance (SPR), and surface-enhanced Raman spectroscopy (SERS). Then, some microfluidics-based systems (valves, traps, and droplets) for single-cell EV analysis are concluded. Finally, we present the challenges and trends of microfluidics in EV research.
- Microfluidic,
- Extracellular vesicle separation,
- Extracellular vesicle analysis,
- Single-cell isolation,
- Single-cell analysis
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沈阳化工大学材料科学与工程学院沈阳 110142