Citation: Weiqing Chen, Richen Hong, Feifang Zhang, Bingcheng Yang. Ion chromatography coupled with a gas-free electrodialytic eluent generator for sugar analysis[J]. Chinese Chemical Letters, ;2026, 37(10): 112528. doi: 10.1016/j.cclet.2026.112528 shu

Ion chromatography coupled with a gas-free electrodialytic eluent generator for sugar analysis

Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

bcyang@ecust.edu.cn

Received Date: 19 August 2025
Revised Date: 12 February 2026
Accepted Date: 13 February 2026
Available Online: 15 February 2026

Figures(5)

The determination and quantification of sugars are important for quality control and assurance of food. Here we present a gas-free electrodialytic eluent generation (EG) platform for sugar analysis by anion exchange chromatography equipped with pulsed amperometric detector. It consists of a gas-free EG for automatic production of highly pure KOH eluent with wide concentration range up to at least 210 mmol/L required for ionization and elution of sugars. The only routine reagent needed is deionized water, driven by a simple isocratic pump to realize isocratic or gradient elution. This platform overcomes many drawbacks associated with common manner of manually prepared KOH eluent driven by gradient pump, and offers an easy way for sugar profiling with improved precision and accuracy. The method was successfully applied to quantification of sugars in biscuits.
- Sugar,
- Ion chromatography,
- Electrodialytic eluent generator,
- Pulsed amperometric detection,
- Eluent preparation,
- Gas-free
1. [1]
  G. Couture, S.E. Cheang, C. Suarez, et al., Nat. Protoc. 19 (2024) 3321–3359. doi: 10.1038/s41596-024-01017-8
2. [2]
  L.F. Li, X. Shi, S.M. Qi, et al., Carbohydr. Polym. 344 (2024) 122533. doi: 10.1016/j.carbpol.2024.122533
3. [3]
  V. Campos, L. Tappy, L. Bally, J.L. Sievenpiper, K.A. Le, Nutr. J. 152 (2022) 1200–1206. doi: 10.1093/jn/nxac039
4. [4]
  A.D. Garcia, V. Leyva, J. Bockova, R.L. Pepino, C. Meinert, Talanta 271 (2024) 125728. doi: 10.1016/j.talanta.2024.125728
5. [5]
  Y. Yuan, J.A. Huang, Y.H. Li, et al., J. Tea Sci. 30 (2010) 435–439.
6. [6]
  Q. Fu, T. Liang, X. Zhang, et al., Carbohydr. Res. 345 (2010) 2690–2697. doi: 10.1016/j.carres.2010.09.033
7. [7]
  F. Dong, M.L. Chen, Y. Zhu, Phys. Test. Chem. Anal. Part B 46 (2010) 76–78.
8. [8]
  Z. Zhang, N.M. Khan, K.M. Nunez, E.K. Chess, C.M. Szabo, Anal. Chem. 84 (2012) 4104–4110. doi: 10.1021/ac300176z
9. [9]
  W.M. Hu, T.Y. Li, Y.L. Yang, S.S. Jia, M. Zhang, Chin. Chem. Lett. 33 (2022) 4808–4816. doi: 10.1016/j.cclet.2022.01.026
10. [10]
  J.J. Li, T.J. Ni, H.Q. Liu, et al., Chin. Chem. Lett. 31 (2020) 1099–1103. doi: 10.1016/j.cclet.2019.11.005
11. [11]
  R.D. Rocklin, C. Pohl, L. Liq. Chromatogr. 6 (1983) 1577–1590. doi: 10.1080/01483918308064876
12. [12]
  M. Mechelke, J. Herlet, J.P. Benz, et al., Anal. Bioanal. Chem. 409 (2017) 7169–7181. doi: 10.1007/s00216-017-0678-y
13. [13]
  T.J. Paskach, H.P. Lieker, P.J. Reilly, K. Thielecke, Carbohydr. Res. 215 (1991) 1–14.
14. [14]
  D.A. Martens, K.L. Loeffelmann, J. Chromatogr. A 1039 (2004) 33–37. doi: 10.1016/j.chroma.2003.12.064
15. [15]
  J. Rohrer, Dionex Technical Note TN 70669: Eluent Preparation For High-Performance Anion-Exchange Chromatogarphy With Pulsed Amperometric Detection, Thermo Fisher Scientific, Sunnyvale, CA, 2017, pp. 1–5.
16. [16]
  Y. Liu, K. Srinivasan, C. Pohl, N. Avdalovic, J. Biochem. Biophy. Methods 60 (2004) 205–232. doi: 10.1016/j.jbbm.2004.01.004
17. [17]
  Y.J. Chen, V. Barreto, A. Woodruff, et al., Anal. Chem. 90 (2018) 10910–10916. doi: 10.1021/acs.analchem.8b02436
18. [18]
  Y.F. Lu, L.T. Zhou, B.C. Yang, S.J. Huang, F.F. Zhang, Anal. Chem. 90 (2018) 12840–12845. doi: 10.1021/acs.analchem.8b03365
19. [19]
  Y. Sun, C. Liao, F. Zhang, Z. Xu, B. Yang, Anal. Chem. 94 (2022) 6924–6929. doi: 10.1021/acs.analchem.2c00604
1. [1]
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2. [2]
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沈阳化工大学材料科学与工程学院沈阳 110142