Citation: Shan Huang, Xinyi Jing, Jin Shao, Xiaoming Ren, Xiaojun Chen. DNAzyme-based sensing tools[J]. Chinese Chemical Letters, ;2026, 37(9): 112451. doi: 10.1016/j.cclet.2026.112451 shu

DNAzyme-based sensing tools

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Molecular Engineering, Jiangsu Provincial University Key Laboratory of Intelligent Medical Sensing Materials and Devices, Nanjing Tech University, Nanjing 211816, China

chenxj@njtech.edu.cn

Received Date: 2 August 2025
Revised Date: 31 December 2025
Accepted Date: 23 January 2026
Available Online: 23 January 2026

Figures(6)

DNAzyme is an artificially created deoxyribonuclease that is produced through in vitro screening. Due to the special properties of DNAzyme, such as catalytic activity, recognition function, excellent thermal stability and flexible designability, it has a broad prospect in constructing biosensors. Many innovative biosensors have been developed by integrating DNAzymes with nanomaterials that exhibit distinct physicochemical properties. This review focuses on the classification and application of DNAzyme-based biosensors, such as RNA-cleavage DNAzyme (8-17, 10-23 DNAzyme), peroxidase mimicking DNAzyme. Finally, some risks and challenges in this field are also discussed.
- RNA-cleavage DNAzyme,
- Peroxidase mimicking DNAzyme,
- Nanomaterials,
- Metal ions,
- Nucleic acid,
- Bacteria
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