Citation: Wenke Liu, Di Zhang, Ziyu Qu, Keke Yi, Shumin Wan, Zihong Ye, Xinhua Dai, Jie Xie, You Jiang, Xiang Fang. Home-built LC-MiniMS system for quantification of tacrolimus in whole blood[J]. Chinese Chemical Letters, ;2026, 37(6): 112109. doi: 10.1016/j.cclet.2025.112109 shu

Home-built LC-MiniMS system for quantification of tacrolimus in whole blood

Wenke Liu ^{a, b, 1} ,
Di Zhang ^{a, 1} ,
Ziyu Qu ^a ,
Keke Yi ^{a, c} ,
Shumin Wan ^{a, b} ,
Zihong Ye ^b ,
Xinhua Dai ^a ,
Jie Xie ^{a, *,} ,
You Jiang ^{a, *,} ,
Xiang Fang ^{a, *,}

a.
Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China
b.
College of Life Sciences, China Jiliang University, Hangzhou 310000, China
c.
School of Physics Science and Engineering, Tongji University, Shanghai 200092, China

xiejie@nim.ac.cn

jiangyou@nim.ac.cn

fangxiang@nim.ac.cn

Received Date: 17 June 2025
Revised Date: 17 September 2025
Accepted Date: 11 November 2025
Available Online: 13 November 2025

Figures(9)

Precise assessment of tacrolimus (TAC) concentrations is critical in clinical diagnostics, and liquid chromatography–mass spectrometry (LC-MS/MS) is the preferred approach due to its high specificity and sensitivity. However, classic LC-MS/MS systems are frequently enormous, costly, and need expert operation, which restricts its applicability in numerous industries. In this paper, a liquid chromatography–miniature mass spectrometry (LC-MiniMS) system was designed and developed. The miniature linear ion trap spectrometer had a footprint of 59 × 38 × 27 cm³, which substantially reduced the instrument size and cost while maintaining quantitative performance. The LC-MiniMS system's circuit boards were integrated and the software automation was optimized, so it was more convenient to use and maintain. Results demonstrated excellent linearity over the range of 0.5–50 ng/mL with R² > 0.99. The limit of detection and limit of quantification were 0.1 and 0.3 ng/mL, respectively. The accuracy ranged from 99.67% to 106.10%, intraday precision was between 0.70% and 2.61%, and interday precision was between 0.90% and 2.90%, all within acceptable limits, and matrix effects were negligible. The method was successfully applied to quantify TAC in 32 clinical whole-blood samples, and the results strongly agreed with those from a conventional LC-MS/MS system (QTRAP 6500+). The LC-MiniMS system can efficiently quantify TAC in whole blood and provide a tiny, cost-effective, and uncomplicated option for therapeutic drug monitoring in clinical settings, especially in decentralized or resource-limited scenarios.
- LC-MiniMS system,
- Tacrolimus,
- Therapeutic drug monitoring,
- Immunosuppressant quantification,
- Clinical diagnostics
1. [1]
  S.J. Li, J. Wang, L. Ma, et al., Cell Res. 26 (2016) 336–349. doi: 10.1038/cr.2016.14
2. [2]
  M. Wang, J. Zhou, Q. Niu, H. Wang, Front. Pharmacol. 15 (2024) 1331800. doi: 10.3389/fphar.2024.1331800
3. [3]
  F.J. Dumont, Curr. Med. Chem. 7 (2000) 731–748. doi: 10.2174/0929867003374723
4. [4]
  O. Campagne, D.E. Mager, K.M. Tornatore, J. Clin. Pharmacol. 59 (2019) 309–325. doi: 10.1002/jcph.1325
5. [5]
  C. Thongprayoon, P. Hansrivijit, K. Kovvuru, et al., J. Clin. Med. 9 (2020) 2193. doi: 10.3390/jcm9072193
6. [6]
  E.J. Kim, S.J. Kim, K.H. Huh, et al., Sci. Rep. 11 (2021) 12114. doi: 10.1038/s41598-021-91630-4
7. [7]
  C. Tron, F. Lemaitre, C. Verstuyft, et al., Clin. Pharmacokinet. 58 (2019) 593–613. doi: 10.1007/s40262-018-0717-7
8. [8]
  D.A. Hesselink, R. Bouamar, L. Elens, R.H.N. van Schaik, T. van Gelder, Clin. Pharmacokinet. 53 (2014) 123–139. doi: 10.1007/s40262-013-0120-3
9. [9]
  M.E. Mohamed, D.P. Schladt, W. Guan, et al., Am. J. Transplant. 19 (2019) 2795–2804. doi: 10.1111/ajt.15385
10. [10]
  A.D. Leino, C. Emoto, T. Fukuda, et al., Am. J. Transplant. 19 (2019) 2944–2948. doi: 10.1111/ajt.15398
11. [11]
  D.R. Migliozzi, N.J. Asal, Ann. Pharmacother. 54 (2020) 171–177. doi: 10.1177/1060028019871891
12. [12]
  E.B. Rangel, Expert Opin. Drug Metab. Toxicol. 10 (2014) 1585–1605. doi: 10.1517/17425255.2014.964205
13. [13]
  H.R. Whalen, J.A. Glen, V. Harkins, et al., Transplantation 101 (2017) 430–436. doi: 10.1097/TP.0000000000001129
14. [14]
  S.C. Ong, R.S. Gaston, Transplantation 105 (2021) 484–495. doi: 10.1097/tp.0000000000003350
15. [15]
  M. Yu, M. Liu, W. Zhang, Y. Ming, Curr. Drug Metab. 19 (2018) 513–522. doi: 10.2174/1389200219666180129151948
16. [16]
  D. Lantigua, J. Trimper, B. Unal, G. Camci-Unal, Lab Chip 21 (2021) 3289–3297. doi: 10.1039/d1lc00473e
17. [17]
  H. Gong, Q. Zeng, S. Gai, et al., Chin. Chem. Lett. 36 (2025) 110059. doi: 10.1016/j.cclet.2024.110059
18. [18]
  P.K. Sharma, V. Mishra, S. Verma, A. Bhatia, Pertanika J. Sci. Tech. 27 (2019) 371–385.
19. [19]
  T.R. Zijp, T.J. Knobbe, K. van Hateren, et al., J. Chromatogr. B 1222 (2023) 123709. doi: 10.1016/j.jchromb.2023.123709
20. [20]
  C. Shu, T.F. Li, D. Li, Z.Q. Li, X.H. Xia, Chin. Chem. Lett. 33 (2022) 916–919. doi: 10.1016/j.cclet.2021.07.009
21. [21]
  T. Shimada, D. Kawakami, A. Fujita, et al., J. Pharm. Health Care Sci. 10 (2024) 5.
22. [22]
  A. Kocur, D. Marszałek, J. Rubik, A. Czajkowska, T. Pawiński, Pharmaceutics 15 (2023) 299. doi: 10.3390/pharmaceutics15010299
23. [23]
  L. Gao, Q. Song, G.E. Patterson, R.G. Cooks, Z. Ouyang, Anal. Chem. 78 (2006) 5994–6002. doi: 10.1021/ac061144k
24. [24]
  L. Li, T.C. Chen, Y. Ren, et al., Anal. Chem. 86 (2014) 2909–2916. doi: 10.1021/ac403766c
25. [25]
  Q. Ma, H. Bai, W. Li, et al., Talanta 142 (2015) 190–196.
26. [26]
  L. Li, Y. Zhang, L. Zhao, et al., Chem. Eng. J. 485 (2024) 149997.
27. [27]
  Y. Zhang, L. Li, J. Li, Q. Ma, Biosens. Bioelectron. 249 (2024) 116010.
28. [28]
  L. Zhou, J. Jiang, K. Zhao, et al., Chin. Chem. Lett. 29 (2018) 707–710.
29. [29]
  H. Gu, G. Dai, S. Liu, et al., Chin. Chem. Lett. 34 (2023) 107715.
30. [30]
  S. Sajjadi, M. Siahi-Shadbad, M.R. Afshar Mogaddam, ImmunoAnalysis 2 (2022) 13. doi: 10.34172/ia.2022.13
31. [31]
  X. Fang, J. Xie, S. Chu, et al., Engineering 16 (2022) 56–64.
32. [32]
  P. Yang, J.S. Chang, J.W. Wong, et al., J. Agric. Food Chem. 63 (2015) 5169–5177. doi: 10.1021/jf505168v
33. [33]
  Z. Ju, X. Guo, L. Li, et al., Anal. Chem. 97 (2025) 712–720. doi: 10.1021/acs.analchem.4c05129
34. [34]
  S. Wang, J. Wu, Q. Wang, et al., J. Pharm. Biomed. Anal. 245 (2024) 116194.
35. [35]
  S. Pandey, Y. Hu, L.R. Bushman, et al., Anal. Bioanal. Chem. 414 (2022) 3387–3395. doi: 10.1007/s00216-022-03954-3
36. [36]
  X. Fan, B. Jiao, X. Zhou, W. Zhang, Z. Ouyang, Anal. Chem. 97 (2025) 9111–9125. doi: 10.1021/acs.analchem.5c01223
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2. [2]
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9. [9]
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11. [11]
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17. [17]
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18. [18]
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