Citation: Xun Xu, Jianwen Wei, Yuan-Fu Ding, Junyan Li, Mian Tang, Chun Wang, Beibei Xie, Qingwen Zhang, Yan Gao. Supramolecularly engineered bacteria reactors mediated cyclic consumption of lactic acid for targeted cancer therapy[J]. Chinese Chemical Letters, ;2026, 37(8): 112105. doi: 10.1016/j.cclet.2025.112105 shu

Supramolecularly engineered bacteria reactors mediated cyclic consumption of lactic acid for targeted cancer therapy

Xun Xu ^{a, 1} ,
Jianwen Wei ^{a, 1} ,
Yuan-Fu Ding ^{b, *,} ,
Junyan Li ^a ,
Mian Tang ^a ,
Chun Wang ^c ,
Beibei Xie ^a ,
Qingwen Zhang ^{a, *,} ,
Yan Gao ^{a, *,}

a.
State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
b.
School of Medical and Information Engineering, Gannan Medical University, Ganzhou 341000, China
c.
College of Biology and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525024, China

yfding@gmu.edu.cn

qwzhang@um.edu.mo

gaoyanki@hotmail.com

Received Date: 8 July 2025
Revised Date: 31 October 2025
Accepted Date: 11 November 2025
Available Online: 12 November 2025

Figures(5)

Lactic acid plays a crucial role in tumor growth, maintenance, and metastasis. Given its significant impact on tumorigenesis, modulating lactic acid levels within the tumor microenvironment (TME) presents new opportunities for cancer therapy. Herein we developed a supramolecularly engineered bacterial reactor using a strong host-guest interaction between cucurbit[7]uril (CB[7])-modified attenuated Salmonella typhimurium VNP20009 (VNP) and adamantane (ADA)-functionalized lactate oxidase (LOX)-loaded liposomes. The VNP-mediated tumor colonization of these reactors facilitated LOX-driven lactic acid depletion in the hypoxic TME, simultaneously producing hydrogen peroxide (H₂O₂). The presence of H₂O₂ triggered the expression of catalase in VNP, which, in turn, catalyzed the conversion of H₂O₂ to oxygen (O₂). This cyclic cascade reactor thus provided a continuous and stable oxygen source, promoting sustained lactic acid consumption for metabolic treatment, ultimately leading to tumor growth suppression and an enhanced anticancer effect.
- Supramolecularly engineered bacteria,
- Cancer metabolic treatment,
- Cyclic lactic acid consumption,
- Tumor colonization,
- Targeted accumulation
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