Citation: Huiling Hou, Pengfei Qi, Haoqing Ren, Hongxia Cui, Xue Zhang, Likun Liu, Haijun Wang, Peng Hou, Song Chen, Mingming Yu. Dual-channel fluorescent probe with large spectral separation for ultrasensitive H₂S detection in food spoilage and arthritis models[J]. Chinese Chemical Letters, ;2026, 37(8): 111800. doi: 10.1016/j.cclet.2025.111800 shu

Dual-channel fluorescent probe with large spectral separation for ultrasensitive H₂S detection in food spoilage and arthritis models

Huiling Hou ^a ,
Pengfei Qi ^b ,
Haoqing Ren ^b ,
Hongxia Cui ^b ,
Xue Zhang ^b ,
Likun Liu ^c ,
Haijun Wang ^b ,
Peng Hou ^b ,
Song Chen ^{b, *,} ,
Mingming Yu ^{d, *,}

a.
Achievement Transformation Center, Qiqihar Medical University, Qiqihar 161006, China
b.
College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China
c.
Research Institute of Medicine & Pharmacy, Qiqihar Medical University, Qiqihar 161006, China
d.
Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China

chensongchemistry@163.com

yumm@zzu.edu.cn

Received Date: 26 June 2025
Revised Date: 6 September 2025
Accepted Date: 7 September 2025
Available Online: 8 September 2025

Figures(6)

Hydrogen sulfide (H₂S), a gaseous signaling molecule with diverse biological functions, exhibits abnormal concentrations that are closely associated with arthritic pathogenesis and serve as a characteristic indicator of protein-rich food spoilage. To address the need for reliable H₂S detection, this study developed a novel fluorescent probe, BHP-PC, by rationally integrating methylene blue and benzothiazole fluorophores. The probe demonstrates remarkable spectral separation (215 nm) and high selectivity, enabling simultaneous monitoring of fluorescence signals in both blue (detection limit: 12.5 nmol/L) and red (detection limit: 22.6 nmol/L) channels for highly sensitive H₂S quantification. BHP-PC exhibits excellent dual-color imaging capabilities in both cellular and zebrafish models. The distinct colorimetric response facilitated the successful development of test strips for practical H₂S detection in spoiled proteinaceous samples. Furthermore, the probe’s application in a λ-carrageenan-induced murine arthritis model enabled real-time H₂S visualization, demonstrating its potential as an analytical tool for arthritis diagnosis and therapeutic monitoring.
- Hydrogen sulfide,
- Dual channel,
- Remarkable spectral separation,
- Food spoilage detection,
- Arthritis
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Dual-channel fluorescent probe with large spectral separation for ultrasensitive H2S detection in food spoilage and arthritis models

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通讯作者: 陈斌, bchen63@163.com

Dual-channel fluorescent probe with large spectral separation for ultrasensitive H₂S detection in food spoilage and arthritis models