Citation: Jinwei Ren, Ruiyun Huo, Xiaoqi Sun, Guobo Guan, Ying Shi, Yangyang Han, Yongsheng Che, Ling Liu. Anti-MRSA anthrone–macrolide hybrids from the ascomycete fungus Neonectria sp.[J]. Chinese Chemical Letters, ;2026, 37(8): 111780. doi: 10.1016/j.cclet.2025.111780 shu

Anti-MRSA anthrone–macrolide hybrids from the ascomycete fungus Neonectria sp.

Jinwei Ren ^{a, 1} ,
Ruiyun Huo ^{a, 1} ,
Xiaoqi Sun ^{a, 1} ,
Guobo Guan ^a ,
Ying Shi ^a ,
Yangyang Han ^c ,
Yongsheng Che ^{c, *,} ,
Ling Liu ^{a, b, *,}

a.
State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
b.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
c.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China

ysche@imb.cams.cn

liul@im.ac.cn

Received Date: 2 June 2025
Revised Date: 15 August 2025
Accepted Date: 1 September 2025
Available Online: 2 September 2025

Figures(4)

Two novel anthrone–macrolide hybrids, neonectones A and B (1 and 2), one new 10-membered macrolide neonectone C (3), and one new oxaphenalenone derivative, (±)-neonectone D (4), together with five known compounds (5–9) were isolated from the crude extract of the ascomycete fungus Neonectria sp. Compounds 1 and 2 represent the first example of anthrone–ten-membered lactone heterodimers, possessing an unprecedented 5-(10-oxo-9,10-dihydroanthracen-9-yl)oxecane-2,7-dione skeleton. Their structures and absolute configurations were established by a combination of extensive spectroscopic analysis, ¹³C nuclear magnetic resonance spectroscopy (NMR) chemical shifts calculations with DP4+ probability analysis and electronic circular dichroism (ECD) calculations. Bioactivity evaluation revealed that compounds 1, 2 and 9 exhibited anti-methicillin-resistant Staphylococcus aureus (MRSA) activity. Compound 9 exhibited anti-MRSA activity through disrupting membrane integrity, suppressing energy and nucleotide metabolism, modulating membrane transport and transcription/translation regulatory factors, as well as inhibiting quorum sensing and biofilm formation.
- Ascomycete fungus,
- Heterodimer,
- Structure elucidation,
- Chemical calculations,
- Anti-MRSA activity
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