Citation: Dou Zhang, Xinyi Cai, Yimin Li, Yong Liu, Long Qiu, Zhenying Diao, Xuyi Liu, Yuta Nishina, Yajuan Zou, Jianbo Sun, Shujing Liang, Daxiang Cui, Ting Yin. Nanozyme with dual enzyme activity mediating cascade catalytic therapy synergize multiple functions for antitumor therapy[J]. Chinese Chemical Letters, ;2026, 37(3): 111569. doi: 10.1016/j.cclet.2025.111569 shu

Nanozyme with dual enzyme activity mediating cascade catalytic therapy synergize multiple functions for antitumor therapy

Dou Zhang ^{a, b} ,
Xinyi Cai ^{a, b} ,
Yimin Li ^c ,
Yong Liu ^{a, b} ,
Long Qiu ^{a, b} ,
Zhenying Diao ^{a, b} ,
Xuyi Liu ^{a, b} ,
Yuta Nishina ^d ,
Yajuan Zou ^d ,
Jianbo Sun ^a ,
Shujing Liang ^{e, *,} ,
Daxiang Cui ^{a, b, f, *,} ,
Ting Yin ^{a, b, *,}

a.
Research Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, School of Pharmacy, Guangdong Medical University, Dongguan 523808, China
b.
Dongguan Research Center for Biomedical Nano Engineering Technology Research, Guangdong Medical University, Dongguan 523808, China
c.
The First Clinical College of Jinan University, Guangzhou 510632, China
d.
Research Institute for Interdisciplinary Science, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama 700-8530, Japan
e.
Department of Oncology, East Hospital Affiliated to Tongji University, Tongji University School of Medicine, Tongji University, Shanghai 200092, China
f.
School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

liangshujing02@126.com

dxcui@sjtu.edu.cn

wsyt132@163.com

Received Date: 29 April 2025
Revised Date: 7 July 2025
Accepted Date: 9 July 2025
Available Online: 10 July 2025

Figures(5)

Nanozymes-mediated catalytic therapy is an emerging approach for tumor treatment, but the performance of the nanozymes is limited by low enzyme catalytic efficiency, low reaction substrate concentration, and immunosuppressive features of the tumor microenvironment. Herein, a hollow gold nanorods loaded with oxygen-carrying hemoglobin (HAuHbO₂) nanozyme with high enzyme catalytic efficiency is reported. In the tumor microenvironment, HAuHbO₂ shows dual enzyme activities of glucose oxidase (GOD) and peroxidase (POD) for spontaneous cascade catalytic reactions to realize chemodynamic therapy (CDT). Meanwhile, it exerts excellent photothermal conversion ability for photothermal therapy (PTT). This study also reports the antitumor ability of HAuHbO₂, including its mechanism of action in vivo. HAuHbO₂ can induce the occurrence of immunogenic cell death (ICD) with the involvement of near-infrared light, which in turn induces the immune response of the organism. Overall, a simple, ingenious, and multifunctional nanozyme is designed in this study, which can provide a basis for applying nanozymes in antitumor therapy.
- Nanozyme,
- Catalytic therapy,
- Chemodynamic therapy,
- Synergistic therapy,
- Immunogenic cell death
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沈阳化工大学材料科学与工程学院沈阳 110142