Citation: Mingyue Luo, Xin Xue, Kehui Zhang, Honghong Rao, Baodui Wang, Zhentong Zhu, Jinhai Fan, Zhonghua Xue. Hollow mesoporous Prussian blue nanozymes with intrinsic self-chromogenicity and photothermal amplification as a pH-adaptive dual-modal biosensor for one-step glucose monitoring[J]. Chinese Chemical Letters, ;2026, 37(3): 111496. doi: 10.1016/j.cclet.2025.111496 shu

Hollow mesoporous Prussian blue nanozymes with intrinsic self-chromogenicity and photothermal amplification as a pH-adaptive dual-modal biosensor for one-step glucose monitoring

Mingyue Luo ^a ,
Xin Xue ^b ,
Kehui Zhang ^c ,
Honghong Rao ^c ,
Baodui Wang ^b ,
Zhentong Zhu ^a ,
Jinhai Fan ^d ,
Zhonghua Xue ^{a, *,}

a.
Key Laboratory of Water Security and Water Environment Protection in Plateau Intersection (NWNU), Ministry of Education, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China
b.
State Key Laboratory of Applied Organic Chemistry and Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou 730000, China
c.
School of Chemical Engineering, Lanzhou City University, Lanzhou 730070, China
d.
Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China

xzh@nwnu.edu.cn

Received Date: 31 March 2025
Revised Date: 19 June 2025
Accepted Date: 19 June 2025
Available Online: 21 June 2025

Figures(5)

Traditional enzyme-nanozymes cascade assays for glucose detection are usually limited by pH incompatibility and operational complexity. Herein, we present a strategy based on hollow mesoporous Prussian blue (HMPB) nanozymes for one-step, dual-modal glucose sensing under neutral conditions. The rationally designed HMPB nanozymes exhibit intrinsic peroxidase-like activity at physiological pH (~7.4), inherent chromogenic properties and superior photothermal conversion efficiency. These features directly enable integration with glucose oxidase (GOx) for one-step glucose detection without intermediate pH adjustment. Additionally, the catalytic coupling of 4-aminoantipyrine/phenol oxidation products, enhanced by the intrinsic blue coloration of HMPB, generates vivid multicolorimetric responses for smartphone-based quantitative analysis. To enhance signal reliability, the photothermal properties of HMPB nanozymes are further ingeniously coupled with the thermal-responsive characteristics of oxidized 3,3′,5,5′-tetramethylbenzidine (oxTMB), establishing a dual-amplified thermal imaging platform through portable infrared thermal imager detection. HMPB nanozymes serve as both a catalytic activator and an intrinsic signal reporter, establish a new platform in dual-modal glucose monitoring. The platform demonstrates remarkable clinical adaptability through its smartphone-compatible colorimetric readout and portable thermal imaging capabilities, achieving a detection limit of 1.39 µmol/L (multicolorimetric modal) and 3.05 µmol/L (photothermometric modal) for glucose with robust reliability in human serum samples. This research overcomes the pH mismatch barrier in enzyme-nanozymes cascade system, and providing a cost-effective, instrument-flexible detection strategy that bridges laboratory research and point-of-care diagnostics.
- Hollow mesoporous Prussian blue nanozymes,
- Multicolorimetric modal,
- Photothermometric modal,
- Enzyme-nanozymes cascade assays,
- One-step detection
1. [1]
  Y. Fu, D. Zeng, J. Chao, et al., J. Am. Chem. Soc. 135 (2013) 696–702. doi: 10.1021/ja3076692
2. [2]
  X. Sun, Y. Li, Q. Yang, et al., Chin. Chem. Lett. 32 (2021) 1780–1784. doi: 10.1016/j.cclet.2020.12.041
3. [3]
  H. Wei, E. Wang, Chem. Soc. Rev. 42 (2013) 6060–6093. doi: 10.1039/c3cs35486e
4. [4]
  S. Li, Z. Chen, F. Yang, W. Yue, Chin. Chem. Lett. 35 (2024) 108793. doi: 10.1016/j.cclet.2023.108793
5. [5]
  N. Ye, S. Huang, H. Yang, et al., Anal. Chem. 93 (2021) 13981–13989. doi: 10.1021/acs.analchem.1c03381
6. [6]
  Y. Liu, R. Lv, S. Sun, et al., Chin. Chem. Lett. 33 (2022) 807–811. doi: 10.1016/j.cclet.2021.06.087
7. [7]
  L. Gao, J. Zhuang, L. Nie, et al., Nat. Nanotechnol. 2 (2007) 577–583. doi: 10.1038/nnano.2007.260
8. [8]
  P. Jin, S. Lin, D. Wang, et al., Chin. Chem. Lett. 36 (2025) 110916. doi: 10.1016/j.cclet.2025.110916
9. [9]
  Y. Chen, Q. Yuchi, T. Li, et al., Sensor. Actuat. B: Chem. 305 (2020) 127436. doi: 10.1016/j.snb.2019.127436
10. [10]
  X. Wang, L. Qin, M. Lin, H. Xing, H. Wei, Anal. Chem. 91 (2019) 10648–10656. doi: 10.1021/acs.analchem.9b01884
11. [11]
  A. Kanwal, B. Saif, A. Muhammad, et al., ACS Appl. Nano Mater. 6 (2023) 4734–4746. doi: 10.1021/acsanm.3c00062
12. [12]
  J. Zhang, J. Wang, J. Liao, et al., ACS Appl. Mater. Inter. 13 (2021) 50236–50245. doi: 10.1021/acsami.1c14831
13. [13]
  Y. He, X. Li, X. Xu, J. Pan, X. Niu, J. Mater. Chem. B 6 (2018) 5750–5755. doi: 10.1039/c8tb01853g
14. [14]
  M.R. Krishnendu, S. Bhagat, V. Jain, D. Mehta, S. Singh, Colloid. Surface B 245 (2025) 114303. doi: 10.1016/j.colsurfb.2024.114303
15. [15]
  R. Zhang, L. Liu, W. Li, X. Luo, F. Wu, Colloid. Surface B 222 (2023) 113125. doi: 10.1016/j.colsurfb.2023.113125
16. [16]
  X. Hai, X. Zhu, K. Yu, et al., Biosens. Bioelectron. 192 (2021) 113544. doi: 10.1016/j.bios.2021.113544
17. [17]
  W. Zhang, S. Hu, J.J. Yin, et al., J. Am. Chem. Soc. 138 (2016) 5860–5865. doi: 10.1021/jacs.5b12070
18. [18]
  G. Fu, Y. Zhu, K. Xu, et al., Anal. Chem. 91 (2019) 13290–13296. doi: 10.1021/acs.analchem.9b04059
19. [19]
  X. Xue, M. Gao, H. Rao, et al., Anal. Chim. Acta 1105 (2020) 197–207. doi: 10.1016/j.aca.2020.01.049
20. [20]
  V. Krishnamoorthy, S. Rajiv, A.C.S. Sustain, Chem. Eng. 5 (2017) 146–152. doi: 10.1021/acssuschemeng.6b01088
21. [21]
  W. Wang, Y. Gang, J. Peng, et al., Adv. Funct. Mater. 32 (2022) 2111727. doi: 10.1002/adfm.202111727
22. [22]
  L. Hu, H. Liao, L. Feng, M. Wang, W. Fu, Anal. Chem. 90 (2018) 6247–6252. doi: 10.1021/acs.analchem.8b00885
23. [23]
  Y.W. Bao, X.W. Hua, H.H. Ran, J. Zeng, F.G. Wu, J. Mater. Chem. B 7 (2019) 296–304. doi: 10.1039/c8tb02404a
24. [24]
  Q. Ye, E. Yuan, J. Shen, et al., Adv. Sci. 10 (2023) 2304149. doi: 10.1002/advs.202304149
25. [25]
  W. Shi, M. He, W. Li, et al., ACS Appl. Nano Mater. 4 (2021) 802–810. doi: 10.1021/acsanm.0c03118
26. [26]
  S. Ning, Z. Zhang, Y. Ren, et al., Adv. Sci. 12 (2025) 2414734. doi: 10.1002/advs.202414734
27. [27]
  R. Yin, W. Guo, H. Wang, et al., Chem. Eng. J. 334 (2018) 2539–2546. doi: 10.1016/j.cej.2017.11.174
28. [28]
  M. Hu, S. Furukawa, R. Ohtani, H. Sukegawa, et al., Angew. Chem. Int. Ed. 51 (2012) 984–988. doi: 10.1002/anie.201105190
29. [29]
  J. Huang, H. Gu, G. Wang, et al., Anal. Chem. 95 (2023) 9107–9115. doi: 10.1021/acs.analchem.3c01594
30. [30]
  T.D. Tran, P.T. Nguyen, T.N. Le, M.I. Kim, Biosens. Bioelectron. 182 (2021) 113187. doi: 10.1016/j.bios.2021.113187
31. [31]
  Y.S. Alqahtani, A.M. Mahmoud, M.M. El-Wekil, Talanta 253 (2023) 124024. doi: 10.1016/j.talanta.2022.124024
32. [32]
  S. Wu, D. Guo, X. Xu, J. Pan, X. Niu, Sensor Actuat. B: Chem. 303 (2020) 127225. doi: 10.1016/j.snb.2019.127225
1. [1]
  Mingyue Luo , Kehui Zhang , Honghong Rao , Jianying Li , Xin Xue , Panpan Sun , Xiaoquan Lu , Zhonghua Xue . A simplified ratiometric fluorescent sensing strategy for enhanced detection of alkaline phosphatase employing Prussian blue nanozymes and commercially available chromogen. Chinese Chemical Letters, 2025, 36(9): 110703-. doi: 10.1016/j.cclet.2024.110703
2. [2]
  Sixin Ai , Wenxiu Li , Huayong Zhu , Yang Wan , Weiying Lin . Viscosity-responsive signal amplification dual-modal probe triggered by cysteine/homocysteine for monitoring diabetic liver damages and repair processes. Chinese Chemical Letters, 2025, 36(3): 109904-. doi: 10.1016/j.cclet.2024.109904
3. [3]
  Xinyu Gu , Jun Yu , Huiyu Sun , Nannan Zhang , Zhengying Wu , Yukou Du . One-step synthesis of trimetallic PdCuNi porous nanoflowers for enhanced alcohol oxidation. Chinese Chemical Letters, 2026, 37(1): 111756-. doi: 10.1016/j.cclet.2025.111756
4. [4]
  Tao Ban , Xi-Yang Yu , Hai-Kuo Tian , Zheng-Qing Huang , Chun-Ran Chang . One-step conversion of methane and formaldehyde to ethanol over SA-FLP dual-active-site catalysts: A DFT study. Chinese Chemical Letters, 2024, 35(4): 108549-. doi: 10.1016/j.cclet.2023.108549
5. [5]
  Lanfang Wang , Jiangnan Lv , Yujia Li , Yanqing Hao , Wenjiao Liu , Hui Zhang , Xiaohong Xu . One-step synthesis of nanowoven ball-like NiS-WS₂ for high-efficiency hydrogen evolution. Chinese Chemical Letters, 2025, 36(1): 109597-. doi: 10.1016/j.cclet.2024.109597
6. [6]
  Xingqun Pu , Rongrong Liu , Yuting Xie , Chenjing Yang , Jingyi Chen , Baoling Guo , Chun-Xia Zhao , Peng Zhao , Jian Ruan , Fangfu Ye , David A Weitz , Dong Chen . One-step preparation of biocompatible amphiphilic dimer nanoparticles with tunable particle morphology and surface property for interface stabilization and drug delivery. Chinese Chemical Letters, 2025, 36(3): 109820-. doi: 10.1016/j.cclet.2024.109820
7. [7]
  Yingtao Zhong , Ziwen Qiu , Yanmei Li , Jiaqi Huang , Zhenming Lu , Renjiang Kong , Ni Yan , Hong Cheng . Nutrients deprivation of biomimetic nanozymes for cascade catalysis triggered and oxidative damage induced tumor eradication. Chinese Chemical Letters, 2025, 36(3): 109846-. doi: 10.1016/j.cclet.2024.109846
8. [8]
  Chenchen Xie , Jun Liao , Yi Li , Yunan Zhang , Zhicheng Xiao , Yun Wang , Ting Chen , Liyan Xiong , Tao Pang , Xiangao Jiang , Feng Zhang , Chuan Zhang , Tingfang Wang . Synergistic anti-inflammatory effect of cascade nanozymes for neural recovery in ischemic stroke. Chinese Chemical Letters, 2026, 37(1): 110956-. doi: 10.1016/j.cclet.2025.110956
9. [9]
  Gangsheng Li , Xiang Yuan , Fu Liu , Zhihua Liu , Xujie Wang , Yuanyuan Liu , Yanmin Chen , Tingting Wang , Yanan Yang , Peicheng Zhang . Three-step synthesis of flavanostilbenes with a 2-cyclohepten-1-one core by Cu-mediated [5 + 2] cycloaddition/decarboxylation cascade. Chinese Chemical Letters, 2025, 36(2): 109880-. doi: 10.1016/j.cclet.2024.109880
10. [10]
  Jingyao Yu , Zhuoran Yang , Jian Jiang , Mengshu Xu , Chunyu Yang , Zhuo Li , Wei Guo . Engineering asymmetric electronic structures in Fe-N-S-C nanozymes for dual enzyme-catalyzed-induced ferroptosis and enhanced antitumor efficacy. Chinese Chemical Letters, 2026, 37(3): 111693-. doi: 10.1016/j.cclet.2025.111693
11. [11]
  Tao Tang , Chen Li , Sipu Li , Zhong Qiu , Tianqi Yang , Beirong Ye , Shaojun Shi , Chunyang Wu , Feng Cao , Xinhui Xia , Minghua Chen , Xinqi Liang , Xinping He , Xin Liu , Yongqi Zhang . One-step constructing advanced N-doped carbon@metal nitride as ultra-stable electrocatalysts via urea plasma under room temperature. Chinese Chemical Letters, 2024, 35(11): 109887-. doi: 10.1016/j.cclet.2024.109887
12. [12]
  Guanghui Lin , Jieyao Chen , Xiaojia Liu , Yitong Lin , Xudong Zhu , Guotao Yuan , Bowen Yang , Shuanshuan Guo , Yue Pan , Jianhua Zhou . Sustained modulation of tumor microenvironment via sorafenib-loaded mesoporous ferromanganese nanozymes for enhanced apoptosis-ferroptosis cancer therapy. Chinese Chemical Letters, 2025, 36(8): 111018-. doi: 10.1016/j.cclet.2025.111018
13. [13]
  Ying Wang , Hong Yang , Caixia Zhu , Qing Hong , Xuwen Cao , Kaiyuan Wang , Yuan Xu , Yanfei Shen , Songqin Liu , Yuanjian Zhang . Cascading oxidoreductases-like nanozymes for high selective and sensitive fluorescent detection of ascorbic acid. Chinese Chemical Letters, 2025, 36(4): 110153-. doi: 10.1016/j.cclet.2024.110153
14. [14]
  Manoj Kumar Sarangi , L․D Patel , Goutam Rath , Sitansu Sekhar Nanda , Dong Kee Yi . Metal organic framework modulated nanozymes tailored with their biomedical approaches. Chinese Chemical Letters, 2024, 35(11): 109381-. doi: 10.1016/j.cclet.2023.109381
15. [15]
  Jiayu Xu , Meng Li , Baoxia Dong , Ligang Feng . Fully fluorinated hybrid zeolite imidazole/Prussian blue analogs with combined advantages for efficient oxygen evolution reaction. Chinese Chemical Letters, 2024, 35(6): 108798-. doi: 10.1016/j.cclet.2023.108798
16. [16]
  Shuaiwen Li , Zihui Chen , Feng Yang , Wanqing Yue . The age of vanadium-based nanozymes: Synthesis, catalytic mechanisms, regulation and biomedical applications. Chinese Chemical Letters, 2024, 35(4): 108793-. doi: 10.1016/j.cclet.2023.108793
17. [17]
  Shaobin He , Xiaoyun Guo , Qionghua Zheng , Huanran Shen , Yuan Xu , Fenglin Lin , Jincheng Chen , Haohua Deng , Yiming Zeng , Wei Chen . Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay. Chinese Chemical Letters, 2025, 36(4): 110096-. doi: 10.1016/j.cclet.2024.110096
18. [18]
  Dou Zhang , Xinyi Cai , Yimin Li , Yong Liu , Long Qiu , Zhenying Diao , Xuyi Liu , Yuta Nishina , Yajuan Zou , Jianbo Sun , Shujing Liang , Daxiang Cui , Ting Yin . Nanozyme with dual enzyme activity mediating cascade catalytic therapy synergize multiple functions for antitumor therapy. Chinese Chemical Letters, 2026, 37(3): 111569-. doi: 10.1016/j.cclet.2025.111569
19. [19]
  Zhimin Song , Zhe Tang , Yu Zhang , Yanru Zhou , Xiaozheng Duan , Yan Du , Chong-Bo Ma . DNA-modulated Mo-Zn single-atom nanozymes: Insights from molecular dynamics simulations to smartphone-assisted biosensing. Chinese Chemical Letters, 2025, 36(10): 110680-. doi: 10.1016/j.cclet.2024.110680
20. [20]
  Jiaxu Wang , Jinxie Zhang , Xiuping Wang , Jingying Wang , Lina Chen , Jiahui Cao , Wei Cao , Siyu Liang , Ping Luan , Ke Zheng , Xiao-Kun Ouyang , Li Gao , Xiaowen Ou , Fan Zhang , Meitong Ou , Lin Mei . CaCO₃-coated hollow mesoporous silica nanoparticles for pH-responsive fungicides release. Chinese Chemical Letters, 2024, 35(12): 109697-. doi: 10.1016/j.cclet.2024.109697

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(22)
HTML views(2)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142