Citation: Shaoqiang Wen, Lang Zhang, Zhan Huang, Meng Chen, Yuzhi Xu, Xing Huang, Zhou Zhang, Xiaoyong Zou, Zong Dai, Si-Yang Liu. Aptamer&MOF-functionalized multichannel paper chip for point-of-care testing of small extracellular vesicle membrane protein profile[J]. Chinese Chemical Letters, ;2026, 37(2): 111333. doi: 10.1016/j.cclet.2025.111333 shu

Aptamer&MOF-functionalized multichannel paper chip for point-of-care testing of small extracellular vesicle membrane protein profile

Shaoqiang Wen ^a ,
Lang Zhang ^{b, c} ,
Zhan Huang ^a ,
Meng Chen ^b ,
Yuzhi Xu ^d ,
Xing Huang ^b ,
Zhou Zhang ^a ,
Xiaoyong Zou ^{a, *,} ,
Zong Dai ^{b, e, *,} ,
Si-Yang Liu ^{b, *,}

a.
School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China
b.
Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China
c.
Institute of Rheumatology and Immunology, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China
d.
Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China
e.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

ceszxy@mail.sysu.edu.cn

daizong@mail.sysu.edu.cn

liusiyang@mail.sysu.edu.cn

Received Date: 19 December 2024
Revised Date: 28 March 2025
Accepted Date: 15 May 2025
Available Online: 16 May 2025

Figures(5)

Small extracellular vesicles (sEVs) membrane protein profile (sEVpp) is a novel biomarker for cancer, and it can reveal the in-depth phenotype information. The point-of-care testing (POCT) of sEVpp holds great significance for mass screening of cancer, so the cost-effective and simple detection methods of sEVpp are urgently demanded. Herein, we constructed a paper-based multichannel sEVpp POCT device (sEVpp-PAD) enabled by functional DNA probes and metal-organic framework (MOF). The core components are aptamer/MOF-modified paper chips. The modified aptamers can immunocapture the sEV expressing corresponding proteins, while the modified MOF can provide abundant sites for aptamer-modification, reduce the nonspecific protein absorption, and act as reference for ratiometric detection. Simply powered by two syringes, the sEVpp-PAD can efficiently capture sEVs expressing corresponding protein from cell culture media and sera. Furthermore, a detection probe (DP) consisted of CD63 aptamer and G-quadruplex was developed for the colorimetric detection of captured sEVs. Utilizing this device, the sEVpp in various hepatocellular carcinoma cell culture medium and, more importantly, in human sera can be accurately determined, only with ＄2 device, ＄0.2 detection reagents and 1.8 h procedure. This simple strategy for sEVpp detection can innovatively promote the POCT and subtyping of cancer based on sEV-related liquid biopsy.
- Small extracellular vesicles,
- Protein profile,
- Point-of-care testing,
- Paper-based analytical device,
- Metal-organic frameworks
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