Citation: Luyao Guan, Zhaoxin Wang, Shengkai Li, Phouphien Keoingthong, Zhuo Chen. CoPt graphitic nanozyme enabled naked-eye identification and colorimetric/fluorescent dual-mode detection of phenylenediamine isomers[J]. Chinese Chemical Letters, ;2026, 37(2): 111323. doi: 10.1016/j.cclet.2025.111323 shu

CoPt graphitic nanozyme enabled naked-eye identification and colorimetric/fluorescent dual-mode detection of phenylenediamine isomers

Luyao Guan ^a ,
Zhaoxin Wang ^a ,
Shengkai Li ^{a, b, **,} ,
Phouphien Keoingthong ^d ,
Zhuo Chen ^{a, c, *,}

a.
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China
b.
Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Key Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical University, Guiyang 550025, China
c.
College of Environmental Science & Engineering, Hunan University, Changsha 410082, China
d.
Postgraduate Office, National University of Laos, Panorama 01000, Laos

^**

lishengkai@gmc.edu.cn

zhuochen@hnu.edu.cn

Received Date: 20 March 2025
Revised Date: 30 April 2025
Accepted Date: 14 May 2025
Available Online: 15 May 2025

Figures(6)

Simultaneous identification and quantitative detection of phenylenediamine (PDA) isomers, including o-phenylenediamine (OPD), m-phenylenediamine (MPD), and p-phenylenediamine (PPD), are essential for environmental risk assessment and human health protection. However, current visual detection methods can only distinguish individual PDA isomers and failed to identify binary or ternary mixtures. Herein, a highly active and ultrastable peroxidase (POD)–like CoPt graphitic nanozyme was used for naked-eye identification and colorimetric/fluorescent (FL) dual-mode quantitative detection of PDA isomers. The CoPt@G nanozyme effectively catalyzed the oxidation of OPD, MPD, PPD, OPD + PPD, OPD + MPD, MPD + PPD and OPD + MPD + PPD into yellow, colorless, lilac, yellow, yellow, wine red and reddish-brown products, respectively, in the presence of H₂O₂. Thus, the MPD, PPD, MPD + PPD and OPD + MPD + PPD were easily identified based on the distinct color of their oxidation products, and the OPD, OPD + PPD, OPD + MPD could be further identified by the additional addition of MPD or PPD. Subsequently, CoPt@G/H₂O₂-, a 3,3′,5,5′-tetramethylbenzidine (TMB)/CoPt@G/H₂O₂-, and MPD/CoPt@G/H₂O₂-enabled colorimetric/FL dual-mode platforms for the quantitative detection of OPD, MPD and PPD were proposed. The experimental results illustrated that the constructed sensing platforms exhibit satisfactory sensitivity, comparable to that reported in previous studies. Finally, the evaluation of PDAs in water samples was realized, yielding satisfactory recoveries. This work expanded the application prospects of nanozymes in assessing environmental risks and protection of human security.
- Copt graphitic nanozyme,
- Phenylenediamine isomers,
- Naked–eye identification,
- Colorimetric detection,
- Fluorescent detection
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