Citation: Yueru Wei, Qi Miao, Miaomiao Zhang, Wenying Zhang, Mengxiao Shi, Rui Liu, Jingjing Su, Pengchao Sun, Yongxing Zhao. PtCu nanozyme integrating single atom Pt and Pt subnanoclusters for the sustained treatment of cutaneous melanoma[J]. Chinese Chemical Letters, ;2025, 36(12): 111164. doi: 10.1016/j.cclet.2025.111164 shu

PtCu nanozyme integrating single atom Pt and Pt subnanoclusters for the sustained treatment of cutaneous melanoma

Yueru Wei ^{a, 1} ,
Qi Miao ^{a, 1} ,
Miaomiao Zhang ^a ,
Wenying Zhang ^a ,
Mengxiao Shi ^a ,
Rui Liu ^a ,
Jingjing Su ^a ,
Pengchao Sun ^{a, b, c, *,} ,
Yongxing Zhao ^{a, b, c, *,}

a.
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China
b.
Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, Zhengzhou 450001, China
c.
Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education of China, Zhengzhou University, Zhengzhou 450001, China

Pengchao_Sun@zzu.edu.cn

zhaoyx@zzu.edu.cn

Received Date: 13 December 2024
Revised Date: 27 March 2025
Accepted Date: 31 March 2025
Available Online: 31 March 2025

Figures(5)

Simultaneously suppressing tumor growth and metastasis is a pivotal strategy in the treatment of cutaneous melanoma (CM). Towards this end, we first developed a novel PtCu nanozyme (PtCu-zyme) integrating single-atom Pt and Pt subnanoclusters, which was further functionalized with triphenylphosphine (TPP) to yield PtCu-TPP and confer the nanozyme mitochondria-targeting capabilities. By combining PtCu-TPP with a hyaluronic acid (HA) analog, isoliquiritigenin-grafted HA (HA-ISL), we later formulated PtCu-TPP loaded microneedles (PtCu-TPP@MNs) for potent CM treatment. Our findings indicated that PtCu-zyme exhibited exceptional oxidative enzyme-like properties and PtCu-TPP@MNs significantly inhibited the tumor growth and pulmonary metastasis. Furthermore, PtCu-TPP@MNs not only prolonged the survival of CM-bearing mice but also retained the nanozymes in the tumor, continually catalyzing reactive oxygen species (ROS) generation for sustained nanocatalytic therapy. In vitro studies revealed that PtCu-TPP specifically localized within mitochondria, increasing ROS levels and causing mitochondrial damage, which in turn enhanced the cytotoxicity towards tumor cells. These findings suggest that PtCu-TPP@MN delivery system holds significant promise for the effective treatment of CM, potentially offering a valuable alternative to existing therapeutic strategies.
- Nanozyme,
- Microneedles,
- Cutaneous melanoma,
- Reactive oxygen species,
- Catalytic therapy
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