Citation: Enjia Zhang, Jiaying Cao, Jianxin Cheng, Gaozhe Cai, Shuyue Jiang, Weiwei Xie, Chunping Jia, Jianlong Zhao, Shilun Feng. Rapid multiplex pathogen detection using 96-channel microfluidic chip with magnetic bead method[J]. Chinese Chemical Letters, ;2026, 37(2): 111109. doi: 10.1016/j.cclet.2025.111109 shu

Rapid multiplex pathogen detection using 96-channel microfluidic chip with magnetic bead method

Enjia Zhang ^{a, b} ,
Jiaying Cao ^c ,
Jianxin Cheng ^{b, d} ,
Gaozhe Cai ^e ,
Shuyue Jiang ^c ,
Weiwei Xie ^f ,
Chunping Jia ^b ,
Jianlong Zhao ^{b, g, *,} ,
Shilun Feng ^{b, *,}

a.
College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
b.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
c.
2020 X-Lab, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
d.
Xiangfu Laboratory, Jiashan 314100, China
e.
School of Microelectronics, Shanghai University, Shanghai 200444, China
f.
Department of Pharmacy, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
g.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

jlzhao@mail.sim.ac.cn

shilun.feng@mail.sim.ac.cn

Received Date: 30 October 2024
Revised Date: 17 January 2025
Accepted Date: 17 March 2025
Available Online: 18 March 2025

Figures(5)

The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission. This study introduces a 96-channel microfluidic chip, fabricated through a molding process, which enables the batch detection of pathogens. It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction, elution, and amplification are completed entirely within the chip. This chip can extract nucleic acids from samples in just 10 min, achieving an extraction efficiency comparable to that of traditional in-tube methods. An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination. This approach allows for the simultaneous detection of 21 common respiratory pathogens, with a detection limit of 10 copies per reaction. Furthermore, applications involving clinical samples demonstrate significant practicality. Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips, this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.
- 96-Channel microfuild chip,
- Multiplex pathogen detection,
- Magnetic bead method,
- Respiratory pathogens
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