Citation: Jiaye Wang, Xiong Yang, Yang Lei, Tianwen Xi, Sijie Wang, Yidan Zhou, Bing Liu, Yu Liu, Hui Yang, Leming Sun. Zinc coordination self-assembly of bacitracin nanoparticles with broadened antibacterial spectrum[J]. Chinese Chemical Letters, ;2026, 37(4): 111086. doi: 10.1016/j.cclet.2025.111086 shu

Zinc coordination self-assembly of bacitracin nanoparticles with broadened antibacterial spectrum

Jiaye Wang ^{a, b, 1} ,
Xiong Yang ^{a, b, 1} ,
Yang Lei ^{a, b} ,
Tianwen Xi ^{a, b} ,
Sijie Wang ^a ,
Yidan Zhou ^a ,
Bing Liu ^{c, *,} ,
Yu Liu ^{d, *,} ,
Hui Yang ^{a, *,} ,
Leming Sun ^{a, b, *,}

a.
School of Life Sciences, Engineering Research Center of Chinese Ministry of Education for Biological Diagnosis, Treatment and Protection Technology and Equipment in Special Environment, Northwestern Polytechnical University, Xi'an 710072, China
b.
Dongguan Sanhang Innovation Institute, Dongguan 523808, China
c.
College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China
d.
Department of Cardiology, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Nanjing 210008, China

hkliubing6698@wzu.edu.cn

lycqx@aliyun.com

kittyyh@nwpu.edu.cn

lmsun@nwpu.edu.cn

Received Date: 16 July 2024
Revised Date: 9 March 2025
Accepted Date: 12 March 2025
Available Online: 12 March 2025

Figures(4)

Bacitracin has been extensively studied for its antibacterial application due to its excellent anti-Gram-positive bacterial properties. However, its application of conventional bacitracin has been limited because of its limited antibacterial activity against Gram-negative bacteria, especially negative bacilli. In this study, we designed and synthesized bacitracin-zinc nanodrugs (BPNDs) through zinc coordination self-assembly of bacitracin, which exhibit potent antibacterial effects not only against Gram-positive bacterial Staphylococcus aureus but also against Escherichia coli, a typical Gram-negative bacillus. The morphological and antimicrobial properties of the self-assembled BPNDs with different molar ratios of bacitracin to zinc ions were investigated. The bacterial biofilm experiments confirmed the biofilm scavenging effect of BPNDs, further expanding the application of this antimicrobial agent. In-depth cell viability experiments indicated that this antimicrobial activity might be related to the penetration of BPNDs into bacterial cell membranes. This study reveals that the zinc-coordinated peptide self-assembly strategy expands the antibacterial spectrum of conventional bacitracin, making it a potential candidate for novel antimicrobial drugs to address the bacterial resistance dilemma and provide stable alternatives for a wide range of biomedical and related industries.
- Self-assembly,
- Bacitracin,
- Nanoparticle,
- Antibacterial,
- Membrane penetration
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