Citation: Yeqi Huang, Yue Gao, Yuxin Lin, Yihao Li, Si Xiong, Xia Guo, Chuanbin Wu, Xin Pan, Wenhao Wang, Ziyu Zhao, Zhengwei Huang. “Connect” or “drag”? The impact of physiologically relevant inorganic ion pairs on the protein corona adsorption onto liposomes[J]. Chinese Chemical Letters, ;2026, 37(2): 111014. doi: 10.1016/j.cclet.2025.111014 shu

“Connect” or “drag”? The impact of physiologically relevant inorganic ion pairs on the protein corona adsorption onto liposomes

Yeqi Huang ^a ,
Yue Gao ^a ,
Yuxin Lin ^a ,
Yihao Li ^a ,
Si Xiong ^b ,
Xia Guo ^b ,
Chuanbin Wu ^{b, c} ,
Xin Pan ^d ,
Wenhao Wang ^{d, *,} ,
Ziyu Zhao ^{b, *,} ,
Zhengwei Huang ^{b, *,}

a.
Department of Pharmacy, College of Pharmacy, Jinan University, Guangzhou 511436, China
b.
State Key Laboratory of Bioactive Molecules and Druggability Assessment, Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs, College of Pharmacy, Jinan University, Guangzhou 511436, China
c.
Jiangmen Wuyi Hospital of Traditional Chinese Medicine, Affiliated Jiangmen Traditional Chinese Medicine Hospital of Jinan University, Jiangmen 529031, China
d.
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China

wangwh37@mail2.sysu.edu.cn

zhaoziyu0515@163.com

huangzhengw@jnu.edu.cn

Received Date: 2 November 2024
Revised Date: 26 February 2025
Accepted Date: 27 February 2025
Available Online: 27 February 2025

Figures(7)

The protein corona formation has been reported to influence the liposomes’ behavioral performance in vivo. Accordingly, the effect of physiologically relevant inorganic ion pairs (sodium chloride, sodium sulfate, magnesium chloride, and magnesium sulfate) was investigated. Bovine serum albumin (BSA) was selected as the model protein. Parameters including particle size and zeta potential were assessed, while various spectroscopic techniques were utilized to elucidate the changes in BSA during its interaction with liposomes. The particle size and light intensity distribution changes indicated that the introduction of inorganic pairs, especially the metal cations, could significantly influence both the adsorption of BSA and the aggregation of particles. Furthermore, spectral characterization elucidated that BSA exhibited more extended peptide chains with enhanced exposure to hydrophobic acid amino residues upon adding ion pairs. Electrostatic adsorption and chelation insertion were proposed as metal ion binding modes and the corresponding BSA corona formation. In the electrostatic adsorption mode, sodium ions can enhance the electrostatic interactions, facilitating the “connection” between BSA and liposomes. Magnesium ions can induce stronger hydrophobic interactions through chelation, effectively “drag” BSA segments into the lipid bilayer. This work highlighted important physiological factors for protein-liposome interaction and provided rational model constructions to lay the foundation for further relevant studies.
- Protein corona,
- Liposomes,
- Inorganic ion,
- Interaction patterns,
- Nanoparticle adsorption profile
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沈阳化工大学材料科学与工程学院沈阳 110142