Citation: Donglei Tian, Yixuan Cao, Xiaoyan Zeng, Xiaoxie Ma, Jun Yin. Fluorescence probe for quantitative detection of enzyme activity based on FRET mechanism of azo quencher[J]. Chinese Chemical Letters, ;2026, 37(12): 110978. doi: 10.1016/j.cclet.2025.110978 shu

Fluorescence probe for quantitative detection of enzyme activity based on FRET mechanism of azo quencher

State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, College of chemistry, Central China Normal University, Wuhan 430079, China

yinj@ccnu.edu.cn

Received Date: 20 August 2024
Revised Date: 18 February 2025
Accepted Date: 19 February 2025
Available Online: 19 February 2025

Figures(6)

Enzymes can catalyze corresponding biochemical reactions efficiently under mild conditions, and plays a crucial role in maintaining normal physiological processes. Therefore, the development of enzyme activity detection methods is of great significance. Fluorescent probes for enzyme activity detection have attracted an increasing interest due to their excellent selectivity, high sensitivity and good cell permeability recently. Especially in the last few years, probes based on Förster resonance energy transfer (FRET) mechanism have been successively used for the quantitative analysis of enzyme activity, which has promoted the application of enzyme activity probes in vivo. In this review, we summarized the recent progress on fluorescence probes for quantitative detection of enzyme activity based on azo-quencher involving FRET mechanism. It concluded the design strategies of fluorescent probes, enzyme-responsiveness, and their application in biological systems. These fluorescence probes show the ability of in situ visualization and can achieve the quantitative detection of enzyme activity. We believe it will be useful for designing in situ enzyme activity fluorescent probes for quantitative analysis in vivo.
- Fluorescent probes,
- Förster resonance energy transfer (FRET),
- Quantitative determination,
- Enzyme activity,
- Visualization
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