Citation: Zixuan Chen, Yafeng Wu, Zhaoyan Tian, Zhaohan Wang, Weiwei Liu, Songqin Liu. A reproducible hybrid membrane for in situ analysis of cell secretions with a wide size range[J]. Chinese Chemical Letters, ;2025, 36(12): 110917. doi: 10.1016/j.cclet.2025.110917 shu

A reproducible hybrid membrane for in situ analysis of cell secretions with a wide size range

Zixuan Chen ^{a, 1} ,
Yafeng Wu ^{a, 1,} ,
Zhaoyan Tian ^b ,
Zhaohan Wang ^a ,
Weiwei Liu ^a ,
Songqin Liu ^a

a.
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
b.
State Key Laboratory for Macromolecule Drugs and Large-scale Manufacturing, School of Pharmaceutical Sciences, Liaocheng University, Liaocheng 252059, China

wuyafeng@seu.edu.cn

Received Date: 6 September 2024
Revised Date: 4 January 2025
Accepted Date: 4 February 2025
Available Online: 10 February 2025

Figures(6)

Outer-surface functionalized solid-state nanochannels have emerged as a new powerful tool for label-free and sensitive detection of biotargets, owing to the unique advantages, such as the target's size is not limited by the nanochannel size, probes on the outer surface are easier to modify and characterize. Despite the advancements, the current outer-surface functionalized nanochannels can only achieve single target detection, which is insufficient for understanding disease pathogenesis and clinical diagnosis. Herein, we develop an ordered mesoporous carbon-silicon/anodic aluminum oxide hybrid membrane (MCS/AAO) with outer surface probes for in situ detecting living cells released secretions with a wide size range (from nano-scale to micron-scale). Due to asymmetric nanochannel structure and charge distribution, the hybrid membrane exhibits cation selectivity and a high ionic current rectification value of 29.21. By taking advantage of this mechanism, different cell secretions can be selectively and sensitively detected through replacing the modified aptamers on the outer surface of hybrid membrane. ATP (adenosine triphosphate), VEGF (vascular endothelial growth factor), and HepG2-MVs (micro vesicles) are chosen as model secretions representing different sizes. The detection limits are 0.64 fmol/L for ATP, 3.31 fg/mL for VEGF, and 5.37 × 10⁴ particles/mL for HepG2-MVs, which was over 10-fold higher than that of commercial assay kits. In addition, the prepared hybrid membrane has exceptional mechanical stability, the detection interface could be regenerated at least 5 times. This work provides a promising platform for in situ detection of cell secretions with different types and sizes by one sensing device and facilitates the clinical diagnosis of secretion-related diseases.
- Hybrid membrane,
- Ionic current rectification,
- Biomarker analysis,
- In situ quantitative detection,
- Cell secretions
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