Citation: Xiujuan Qiao, Zhenying Xu, Zhen Wei, Yiting Hou, Fengxian Gao, Xijuan Yu, Xiliang Luo. A wearable electrochemical biosensor based on antifouling and conducting polyaniline hydrogel for cortisol detection in sweat[J]. Chinese Chemical Letters, ;2025, 36(11): 110884. doi: 10.1016/j.cclet.2025.110884 shu

A wearable electrochemical biosensor based on antifouling and conducting polyaniline hydrogel for cortisol detection in sweat

Xiujuan Qiao ^{a, 1} ,
Zhenying Xu ^{b, 1} ,
Zhen Wei ^a ,
Yiting Hou ^a ,
Fengxian Gao ^{c, *,} ,
Xijuan Yu ^a ,
Xiliang Luo ^{a, *,}

a.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Qingdao University of Science and Technology, Qingdao 266042, China
b.
College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China
c.
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China

gaofx@ustb.edu.cn

xiliangluo@qust.edu.cn

Received Date: 17 August 2024
Revised Date: 11 December 2024
Accepted Date: 19 January 2025
Available Online: 20 January 2025

Figures(8)

In this study, we presented a wearable electrochemical sensor for accurate and reliable cortisol detection in sweat. The sensor was built upon a novel platform by combination of conducting polyaniline (PANI) hydrogel and hydrophilic polypeptides, endowing the sensor with superior antifouling property. PANI hydrogel's distinctive water storage characteristic and the attachment of numerous antifouling peptides (Pep) effectively prevent nonspecific adsorption in complex human sweat environment. This innovative configuration significantly enhanced the accuracy of cortisol detection in complex sweat samples. The prepared biosensor was able to achieve reliable cortisol detection in both buffer solution and artificial sweat, covering a detection concentration range from 10⁻¹⁰ to 10^–6 g/mL, with the minimum detection limitation of 33 pg/mL. And this electrochemical biosensor demonstrated outstanding selectivity, excellent stability, and good reproducibility. Notably, the cortisol levels were measured in volunteers during both morning and evening. The observed data exhibited distinct circadian rhythm, consistenting with the results gained from commercially available enzyme-linked immunosorption (ELISA) kit. This wearable biosensor shows giant potential for monitoring cortisol levels in human sweat, enabling real-time evaluation for mental and stress state.
- Wearable electrochemical sensors,
- Antifouling,
- Hydrogel,
- Peptide,
- Cortisol,
- Sweat
1. [1]
  A. Zivony, Nat. Hum. Behav. 3 (2019) 1037-1037. doi: 10.1038/s41562-019-0735-y
2. [2]
  M. Kivimäki, A. Bartolomucci, I. Kawachi, Nat. Rev. Endocrinol. 19 (2023) 10–27. doi: 10.1038/s41574-022-00746-8
3. [3]
  G. Russell, S. Lightman, Nat. Rev. Endocrinol. 15 (2019) 525–534. doi: 10.1038/s41574-019-0228-0
4. [4]
  E. Dziurkowska, M. Wesolowski, J. Clin. Med. 10 (2021) 5204. doi: 10.3390/jcm10215204
5. [5]
  A.J. Steckl, P. Ray, ACS Sens. 3 (2018) 2025–2044. doi: 10.1021/acssensors.8b00726
6. [6]
  E. Mansour, W. Saliba, Y.Y. Broza, et al., ACS Sens. 8 (2023) 3215–3224. doi: 10.1021/acssensors.3c00945
7. [7]
  N.K. Singh, S. Chung, M. Sveiven, et al., ACS Omega 6 (2021) 27888–27897. doi: 10.1021/acsomega.1c03552
8. [8]
  J.E. An, K.H. Kim, S.J. Park, et al., ACS Sens. 7 (2022) 99–108. doi: 10.1021/acssensors.1c01734
9. [9]
  M. Kivimäki, A. Steptoe, Nat. Rev. Cardiol. 15 (2018) 215–229. doi: 10.1038/nrcardio.2017.189
10. [10]
  M.S. Sagmeister, L. Harper, R.S. Hardy, Front. Endocrinol. 13 (2023) 1075809. doi: 10.3389/fendo.2022.1075809
11. [11]
  K. Di, J. Wei, L. Ding, et al., Chin. Chem. Lett. 36 (2025) 109911. doi: 10.1016/j.cclet.2024.109911
12. [12]
  L. Xu, X. Hu, S. Zhou, et al., Chin. Chem. Lett. 35 (2024) 109103. doi: 10.1016/j.cclet.2023.109103
13. [13]
  J. Min, J. Tu, C. Xu, et al., Chem. Rev. 123 (2023) 5049–5138. doi: 10.1021/acs.chemrev.2c00823
14. [14]
  X. Weng, Z. Fu, C. Zhang, et al., Anal. Chem. 94 (2022) 3526–3534. doi: 10.1021/acs.analchem.1c04508
15. [15]
  S. Madhu, A.J. Anthuuvan, S. Ramasamy, et al., ACS Appl. Electron. Mater. 2 (2020) 499–509. doi: 10.1021/acsaelm.9b00730
16. [16]
  T. Laochai, J. Yukird, N. Promphet, et al., Biosens. Bioelectron. 203 (2022) 114039. doi: 10.1016/j.bios.2022.114039
17. [17]
  M. Zea, F.G. Bellagambi, H. Ben Halima, et al., TrAC-trend, Anal. Chem. 132 (2020) 116058.
18. [18]
  M. Bariya, H.Y.Y. Nyein, A. Javey, Nat. Electron. 1 (2018) 160–171. doi: 10.1038/s41928-018-0043-y
19. [19]
  X. Wang, A. He, B. Yu, et al., Anal. Chem. 94 (2022) 14402–14409. doi: 10.1021/acs.analchem.2c03158
20. [20]
  K. Koren, C.M. McGraw, ACS Sens. 8 (2023) 2432–2439. doi: 10.1021/acssensors.3c00961
21. [21]
  L. Tao, Y. Kong, Y. Xiang, et al., Chin. Chem. Lett. 34 (2023) 107481. doi: 10.1016/j.cclet.2022.04.079
22. [22]
  X. She, X. Wang, P. Niu, et al., Chem. Eng. J. 431 (2022) 133354. doi: 10.1016/j.cej.2021.133354
23. [23]
  K. Van Hoovels, X. Xuan, M. Cuartero, et al., ACS Sens. 6 (2021) 3496–3508. doi: 10.1021/acssensors.1c01403
24. [24]
  D. Sim, M.C. Brothers, J.M. Slocik, et al., Adv. Sci. 9 (2022) 2104426. doi: 10.1002/advs.202104426
25. [25]
  H. Zhao, X. Zhang, Y. Qin, et al., Adv. Funct. Mater. 33 (2023) 2212083. doi: 10.1002/adfm.202212083
26. [26]
  S. Upasham, N.K.M. Churcher, P. Rice, et al., ACS Sens. 6 (2021) 659–672. doi: 10.1021/acssensors.0c02622
27. [27]
  Z.Q. Wu, X.Q. Cao, Y. Hua, et al., Anal. Chem. 96 (2024) 3087–3095.
28. [28]
  K. Jayakumar, A. Lielpetere, D.A. Domingo-Lopez, et al., Biosens. Bioelectron. 219 (2023) 114815. doi: 10.1016/j.bios.2022.114815
29. [29]
  M.J. Russo, M. Han, P.E. Desroches, et al., ACS Sens. 6 (2021) 1482–1507. doi: 10.1021/acssensors.1c00390
30. [30]
  X. Qiao, Y. Cai, Z. Kong, et al., ACS Sens. 8 (2023) 2834–2842. doi: 10.1021/acssensors.3c00778
31. [31]
  X. Qiao, Z.H. Qian, W. Sun, et al., Anal. Chem. 95 (2023) 11091–11098. doi: 10.1021/acs.analchem.3c01843
32. [32]
  S. Zhao, X. Qiao, M. Chen, et al., ACS Sens. 7 (2022) 1740–1746. doi: 10.1021/acssensors.2c00518
33. [33]
  J. Li, L. Zhang, P. Huang, et al., Chin. Chem. Lett. 36 (2025) 109970. doi: 10.1016/j.cclet.2024.109970
34. [34]
  C. Jiang, G. Wang, R. Hein, et al., Chem. Rev. 120 (2020) 3852–3889. doi: 10.1021/acs.chemrev.9b00739
35. [35]
  Z. Song, R. Li, X. Yang, et al., Chin. Chem. Lett. 34 (2023) 108314. doi: 10.1016/j.cclet.2023.108314
36. [36]
  C.F. Sharpley, K.G. Kauter, J.R. McFarlane, Clin. Med. Insights-En. 3 (2010) CMED.S4350, doi:10.4137/cmed.s4350. doi: 10.4137/cmed.s4350
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2. [2]
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6. [6]
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7. [7]
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8. [8]
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9. [9]
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10. [10]
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11. [11]
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15. [15]
  Jian Li , Jinjin Chen , Qi-Long Hu , Zhen Wang , Xiao-Feng Xiong . Recent progress of chemical methods for lysine site-selective modification of peptides and proteins. Chinese Chemical Letters, 2025, 36(5): 110126-. doi: 10.1016/j.cclet.2024.110126
16. [16]
  Yang Xu , Le Ma , Yang Wang , Chunmeng Shi . Engineering strategies of biomaterial-assisted exosomes for skin wound repair: Latest advances and challenges. Chinese Chemical Letters, 2025, 36(1): 109766-. doi: 10.1016/j.cclet.2024.109766
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  Yue Sun , Yingnan Zhu , Jiahang Si , Ruikang Zhang , Yalan Ji , Jinjie Fan , Yuze Dong . Glucose-activated nanozyme hydrogels for microenvironment modulation via cascade reaction in diabetic wound. Chinese Chemical Letters, 2025, 36(4): 110012-. doi: 10.1016/j.cclet.2024.110012
18. [18]
  Ruijun Song , Huixu Xie , Guiting Liu . Advances of MXene-based hydrogels for chronic wound healing. Chinese Chemical Letters, 2025, 36(7): 110442-. doi: 10.1016/j.cclet.2024.110442
19. [19]
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