Citation: Jiayin Li, Linan Yang, Feng Shi, Yan Long, Yuru Wang, Daniel D. Stuart, Hongbo Li, Quan Cheng, Lingfeng Min, Zhanjun Yang, Juan Li. Versatile Au nanozyme Raman probe strategy for ultrasensitive encoded photonic crystal-based SERS multiplex immunosensing[J]. Chinese Chemical Letters, ;2025, 36(11): 110883. doi: 10.1016/j.cclet.2025.110883 shu

Versatile Au nanozyme Raman probe strategy for ultrasensitive encoded photonic crystal-based SERS multiplex immunosensing

Jiayin Li ^{a, 1} ,
Linan Yang ^{a, 1} ,
Feng Shi ^{a, 1} ,
Yan Long ^a ,
Yuru Wang ^a ,
Daniel D. Stuart ^c ,
Hongbo Li ^d ,
Quan Cheng ^c ,
Lingfeng Min ^{b, *,} ,
Zhanjun Yang ^{a, *,} ,
Juan Li ^{a, *,}

a.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
b.
Department of pulmonary and critical care medicine, Northem Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou 225001, China
c.
Department of Chemistry, University of California, Riverside, Riverside, CA 92521, United States
d.
School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng 224051, China

minlingfeng@126.com

zjyang@yzu.edu.cn

lijuan@yzu.edu.cn

Received Date: 12 August 2024
Revised Date: 24 December 2024
Accepted Date: 19 January 2025
Available Online: 20 January 2025

Figures(6)

Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy, precision, and efficiency. A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensitive multiplex surface-enhanced Raman scattering (SERS) immunosensor using encoded silica photonic crystal beads (SPCBs). The efficient Au nanozyme Raman probe strategy was constructed using a robust Au nanozyme with high dual enzyme-like activity and SERS activity. On the one hand, Au nanozyme tags with oxidase-like activity can catalyze the oxidation of Raman-inactive 3,3′,5,5′-tetramethylbenzidine (TMB) to Raman-active oxidized TMB (ox-TMB) in the presence of O₂. On the other hand, Au nanozyme tags with peroxidase-like activity can catalyze Raman-inactive TMB to Raman-active ox-TMB in the presence of H₂O₂. This dual catalysis action results in many Raman-active reporter molecules (ox-TMB) enabling highly sensitive detection. Meanwhile, the Au nanozyme as an extraordinary SERS substrate further enhances the detection signals of these Raman reporter molecules. Using reflection peaks of different SPCBs to encode tumor markers, an ultrasensitive multiplex SERS immunosensor was developed for detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP), which exhibited wide linear ranges of 0.001–100 ng/mL for CEA and 0.01–1000 ng/mL for AFP, accompanied by low detection limits of 0.66 pg/mL for CEA and 9.5 pg/mL for AFP, respectively. This work demonstrates a universal and promising nanozyme Raman probe strategy to develop ultrasensitive multiplex SERS immunosensors for precise clinical diagnosis of disease.
- Dual enzyme-like Au nanozyme,
- SERS,
- Multiplex immunosensing,
- Signal amplification,
- Photonic crystal beads
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