Citation: Tiancong Shi, Xi Chen, Xiao Zhou, Hongyi Zhang, Fuping Han, Lihan Cai, Wen Sun, Jianjun Du, Jiangli Fan, Xiaojun Peng. Azaindole-based asymmetric pentamethine cyanine dye for mitochondrial pH detection and near-infrared ratiometric fluorescence imaging of mitophagy[J]. Chinese Chemical Letters, ;2025, 36(6): 110408. doi: 10.1016/j.cclet.2024.110408 shu

Azaindole-based asymmetric pentamethine cyanine dye for mitochondrial pH detection and near-infrared ratiometric fluorescence imaging of mitophagy

Tiancong Shi ^{a, 1} ,
Xi Chen ^{a, 1} ,
Xiao Zhou ^a ,
Hongyi Zhang ^a ,
Fuping Han ^a ,
Lihan Cai ^a ,
Wen Sun ^{a, b} ,
Jianjun Du ^{a, b, *,} ,
Jiangli Fan ^{a, b, *,} ,
Xiaojun Peng ^a

a.
State Key Laboratory of Fine Chemicals, Frontier Science Center for Smart Materials, Dalian University of Technology, Dalian 116024, China
b.
Ningbo Institute of Dalian University of Technology, Ningbo 315016, China

dujj@dlut.edu.cn

fanjl@dlut.edu.cn

Received Date: 8 July 2024
Revised Date: 30 August 2024
Accepted Date: 3 September 2024
Available Online: 3 September 2024

Figures(4)

Mitochondria are crucial organelles responsible for maintaining cell growth, and their homeostasis is closely linked to pH regulation. Physiologically, mitochondria exhibit a weakly alkaline state (pH~8.0). However, when subjected to stress stimuli that cause damage, cells initiate the process of mitophagy, resulting in mitochondrial acidification. Therefore, monitoring changes in mitochondrial pH to comprehend the physiological processes associated with mitophagy is essential. In this study, we developed an asymmetric pentamethine cyanine dye Cy5.5-H-CyN as a probe for continuous monitoring of mitophagy in living cells. By incorporating an azaindole structure into the dye molecule, a ratiometric fluorescence response was achieved that is specifically responsive to pH variations while preserving its ability to target mitochondria and emit near-infrared fluorescence. Through various methods inducing mitophagy, Cy5.5-H-CyN was employed to determine mitochondrial pH quantitatively, demonstrating its suitability as an ideal probe for continuous monitoring of mitophagy in living cells.
- Asymmetric cyanine dye,
- pH probe,
- Mitochondria-targeting,
- Ratiometric fluorescence,
- Mitophagy
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