Citation: Kun Liu, Yulin Cong, Xiongfeng Luo, Meicun Yao, Zhiyong Xie, Hao Li. Utilizing bivalent aptamers as first DNA agonist to activate RTKs heterodimer of different families[J]. Chinese Chemical Letters, ;2025, 36(1): 109839. doi: 10.1016/j.cclet.2024.109839 shu

Utilizing bivalent aptamers as first DNA agonist to activate RTKs heterodimer of different families

Kun Liu ¹ ,
Yulin Cong ¹ ,
Xiongfeng Luo ,
Meicun Yao ^*, ,
Zhiyong Xie ^*, ,
Hao Li ^*,

School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China

Lssymc@mail.sysu.edu.cn

xiezhy@mail.sysu.edu.cn

lihao258@mail.sysu.edu.cn

Received Date: 4 February 2024
Revised Date: 1 March 2024
Accepted Date: 27 March 2024
Available Online: 28 March 2024

Figures(4)

Heterodimerization in RTKs is of vital importance in the RTK signaling and cell functions. Heterodimerization between RTKs can result in diversity of downstream signals, increasing the ability of cells to respond to external experiments. Traditional RTKs heterodimerization always occur in the same families and is lack of agonists to activate the heterodimeric RTKs signaling pathway. Herein, we developed the DNA agonist based on bivalent aptamers for the heterodimerized RTKs of different families, AF/AM-1, which could simultaneously activate FGFR1 and c-Met signaling. It is the first agonist that realizing the heterodimerization and activation of FGFR1 and c-Met, two different RTK families. The activation of FGFR1/c-Met heterodimer result in the down-stream signals transduction, such as the phosphorylation of Akt and Erk, inducing the cell migration and proliferation. The DNA agonist for RTK heterodimer of different families would have potential applications in the fields of biomedicine.
- DNA Agonist,
- Bivalent aptamers,
- RTKs heterodimer
1. [1]
  J. Li, L. Wang, J. Tian, et al., Chem. Soc. Rev. 49 (2020) 1545–1568. doi: 10.1039/c9cs00473d
2. [2]
  Z. Du, C.M. Lovly, Mol. Cancer 17 (2018) 58. doi: 10.1186/s12943-018-0782-4
3. [3]
  M.A. Lemmon, J. Schlessinger, Cell 141 (2010) 1117–1134.
4. [4]
  L. Chen, L. Fu, J. Sun, et al., Nature 618 (2023) 862–870. doi: 10.1038/s41586-023-06155-9
5. [5]
  M.D. Paul, K. Hristova, Chem. Rev. 119 (2019) 5881–5921. doi: 10.1021/acs.chemrev.8b00467
6. [6]
  A. Chausovsky, H. Waterman, M. Elbaum, et al., Oncogene 19 (2000) 878–888. doi: 10.1038/sj.onc.1203410
7. [7]
  J.L. Macdonald-Obermann, L.J. Pike, J. Biol. Chem. 289 (2014) 26178–26188. doi: 10.1074/jbc.M114.586826
8. [8]
  M. Landau, N. Ben-Tal, Biochim. Biophys. Acta 1785 (2008) 12–31.
9. [9]
  M.A. Olayioye, R.M. Neve, H.A. Lane, et al., EMBO J. 19 (2000) 3159–3167. doi: 10.1093/emboj/19.13.3159
10. [10]
  Y. Yarden, M.X. Sliwkowski, Nat. Rev. Mol. Cell. Biol. 2 (2001) 127–137. doi: 10.1038/35052073
11. [11]
  K. Sakamoto, Y. Kawata, Y. Masuda, et al., Biochem. Biophys. Res. Commun. 480 (2016) 55–60. doi: 10.1016/j.bbrc.2016.10.009
12. [12]
  Y. Du, Y. Lyu, J. Lin, et al., Nat. Nanotechnol. 18 (2023) 818–827. doi: 10.1038/s41565-023-01333-2
13. [13]
  D.Y. Zhang, A.J. Turberfield, B. Yurke, et al., Science 318 (2007) 1121–1125. doi: 10.1126/science.1148532
14. [14]
  X. Mao, M. Liu, Q. Li, et al., JACS Au 2 (2022) 2381–2399. doi: 10.1021/jacsau.2c00292
15. [15]
  Y.J. Chen, B. Groves, R.A. Muscat, et al., Nat. Nanotechnol. 10 (2015) 748–760. doi: 10.1038/nnano.2015.195
16. [16]
  Y. Tang, Z. Wang, X. Yang, et al., Chem. Sci. 6 (2015) 5729–5733. doi: 10.1039/C5SC01870F
17. [17]
  M. You, L. Peng, N. Shao, et al., J. Am. Chem. Soc. 136 (2014) 1256–1259. doi: 10.1021/ja4114903
18. [18]
  R. Nutiu, Y. Li, J. Am. Chem. Soc. 125 (2003) 4771–4778. doi: 10.1021/ja028962o
19. [19]
  J. Liu, Z. Cao, Y. Lu, Chem. Rev. 109 (2009) 1948–1998. doi: 10.1021/cr030183i
20. [20]
  N. Nakatsuka, K.A. Yang, J.M. Abendroth, et al., Science 362 (2018) 319–324. doi: 10.1126/science.aao6750
21. [21]
  W. Tan, M.J. Donovan, J. Jiang, Chem. Rev. 113 (2013) 2842–2862. doi: 10.1021/cr300468w
22. [22]
  K. Zhang, H. Gao, R. Deng, et al., Angew. Chem. Int. Ed. 58 (2019) 4790–4799. doi: 10.1002/anie.201809006
23. [23]
  K. Zhang, R. Deng, Y. Sun, et al., Chem. Sci. 8 (2017) 7098–7105. doi: 10.1039/C7SC02489D
24. [24]
  H. Li, M. Wang, T. Shi, et al., Angew. Chem. Int. Ed. 57 (2018) 10226–10230. doi: 10.1002/anie.201806155
25. [25]
  H. Li, J. Gao, L. Cao, et al., Angew. Chem. Int. Ed. 60 (2021) 26087–26095. doi: 10.1002/anie.202108210
26. [26]
  R. Ueki, A. Ueki, N. Kanda, et al., Angew. Chem. Int. Ed. 55 (2016) 579–582. doi: 10.1002/anie.201508572
27. [27]
  H. Liang, Z. Yan, Y. Tong, et al., Chem. Commun. 59 (2023) 1621–1624. doi: 10.1039/d2cc06176g
28. [28]
  M. Wang, F. He, H. Li, et al., Nano. Lett. 19 (2019) 2603–2613. doi: 10.1021/acs.nanolett.9b00421
29. [29]
  L. Wang, H. Liang, J. Sun, et al., J. Am. Chem. Soc. 141 (2019) 12673–12681. doi: 10.1021/jacs.9b05123
30. [30]
  R. Ueki, S. Sando, Chem. Commun. 50 (2014) 13131–13134. doi: 10.1039/C4CC06016D
31. [31]
  R. Ueki, S. Atsuta, A. Ueki, et al., Chem. Commun. 55 (2019) 2672–2675. doi: 10.1039/c8cc08080a
32. [32]
  D.L. Wang, Y.L. Song, Z. Zhu, et al., Biochem. Biophys. Res. Commun. 453 (2014) 681–685. doi: 10.1016/j.bbrc.2014.09.023
1. [1]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
2. [2]
  Zhe Li , Haozhi Lei , Zhiqiang Ren , Cheng Wang , Qian Xia , Weihong Tan . Enhancing the stability of ⁶⁸Ga-labeled RNA aptamers for pancreatic β-cell and insulinoma imaging through nucleoside modifications. Chinese Chemical Letters, 2025, 36(10): 110804-. doi: 10.1016/j.cclet.2024.110804
3. [3]
  Li Xiang , Jingchun Shi , Zifan Zhu , Yi Ru , Lin Peng , Wei Wang , Hongzhi Zhao , Runhui Liu , Yujie Pu , Lei He , Li Wang , Zongwei Cai . Lipidomics study of the regulatory effects of PPARδ agonist GW501516 in plasma of db/db diabetic mice. Chinese Chemical Letters, 2025, 36(11): 110910-. doi: 10.1016/j.cclet.2025.110910
4. [4]
  Leyuan Sun , Xiaoyu Xie , Fangfang Chen . 敦煌壁画的“DNA变身”. University Chemistry, 2025, 40(8): 211-217. doi: 10.12461/PKU.DXHX202410079
5. [5]
  Jianqiu Li , Yi Zhang , Songen Liu , Jie Niu , Rong Zhang , Yong Chen , Yu Liu . Cucurbit[8]uril-based non-covalent heterodimer realized NIR cell imaging through topological transformation from nanowire to nanorod. Chinese Chemical Letters, 2024, 35(10): 109645-. doi: 10.1016/j.cclet.2024.109645
6. [6]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
7. [7]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
8. [8]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
9. [9]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
10. [10]
  Zhongyu Wang , Lijun Wang , Huaixin Zhao . DNA-based nanosystems to generate reactive oxygen species for nanomedicine. Chinese Chemical Letters, 2024, 35(11): 109637-. doi: 10.1016/j.cclet.2024.109637
11. [11]
  Jiangshan Xu , Weifei Zhang , Zhengwen Cai , Yong Li , Long Bai , Shaojingya Gao , Qiang Sun , Yunfeng Lin . Tetrahedron DNA nanostructure/iron-based nanomaterials for combined tumor therapy. Chinese Chemical Letters, 2024, 35(11): 109620-. doi: 10.1016/j.cclet.2024.109620
12. [12]
  Xiaolong Li , Changjiang Li , Chaopeng Shi , Jiarun Wang , Bei Yan , Xianjin Xiao , Tongbo Wu . CRISPR-Cas systems in DNA functional circuits: Strategies, challenges, prospects. Chinese Chemical Letters, 2025, 36(7): 110507-. doi: 10.1016/j.cclet.2024.110507
13. [13]
  Le Yang , Hongye Wei , Zhihe Qing , Linlin Wu . AuNP@DNA nanoflares: Preparation and application in bioanalysis and biomedicine. Chinese Chemical Letters, 2025, 36(8): 110524-. doi: 10.1016/j.cclet.2024.110524
14. [14]
  Haozhi Lei , Qian Xia , Xiqiu Wang , Yang Sun , Weihong Tan . Simulation of immune signal transduction through DNA strand displacement. Chinese Chemical Letters, 2025, 36(12): 110941-. doi: 10.1016/j.cclet.2025.110941
15. [15]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
16. [16]
  Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
17. [17]
  Xiaofen GUAN , Yating LIU , Jia LI , Yiwen HU , Haiyuan DING , Yuanjing SHI , Zhiqiang WANG , Wenmin WANG . Synthesis, crystal structure, and DNA-binding of binuclear lanthanide complexes based on a multidentate Schiff base ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2486-2496. doi: 10.11862/CJIC.20240122
18. [18]
  Yanfei Liu , Yaqin Hu , Yifu Tan , Qiwen Chen , Zhenbao Liu . Tumor acidic microenvironment activatable DNA nanostructure for precise cancer cell targeting and inhibition. Chinese Chemical Letters, 2025, 36(1): 110289-. doi: 10.1016/j.cclet.2024.110289
19. [19]
  Fanghua Zhang , Yuyan Li , Hongyan Zhang , Wendong Liu , Zhe Hao , Mingzheng Shao , Ruizhong Zhang , Xiyan Li , Libing Zhang . Logically integrating exo/endogenous gated DNA trackers for precise microRNA imaging via synergistic manipulation. Chinese Chemical Letters, 2025, 36(1): 109848-. doi: 10.1016/j.cclet.2024.109848
20. [20]
  Yao HUANG , Yingshu WU , Zhichun BAO , Yue HUANG , Shangfeng TANG , Ruixue LIU , Yancheng LIU , Hong LIANG . Copper complexes of anthrahydrazone bearing pyridyl side chain: Synthesis, crystal structure, anticancer activity, and DNA binding. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 213-224. doi: 10.11862/CJIC.20240359

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(1607)
HTML views(184)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142