Citation: Ruixin Liu, Feng Shi, Yanping Xia, Haibing Zhu, Jiawen Cao, Kai Peng, Chuanli Ren, Juan Li, Zhanjun Yang. Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay[J]. Chinese Chemical Letters, ;2024, 35(11): 109664. doi: 10.1016/j.cclet.2024.109664 shu

Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay

Ruixin Liu ^{a, 1} ,
Feng Shi ^{a, 1} ,
Yanping Xia ^{a, 1} ,
Haibing Zhu ^a ,
Jiawen Cao ^a ,
Kai Peng ^a ,
Chuanli Ren ^{b, *,} ,
Juan Li ^{a, *,} ,
Zhanjun Yang ^{a, *,}

a.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
b.
Clinical Medical College of Yangzhou University, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, Yangzhou University, Yangzhou 225001, China

renchl@163.com

lijuan@yzu.edu.cn

zjyang@yzu.edu.cn

Received Date: 28 October 2023
Revised Date: 27 January 2024
Accepted Date: 19 February 2024
Available Online: 28 February 2024

Figures(6)

Label-free immunoassay is confronted with a great challenge that its insufficient sensitivity for low concentration analytes, which can be assigned to the low catalytic efficiency of modified materials towards electroactive molecules. Herein, a universal MOF nanozyme-induced catalytic amplification strategy was proposed for constructing highly sensitive label-free electrochemical immunoassay. Specifically, the synthesized CuFe-MOF nanozyme with superior peroxidase (POD)-like activity, regarding as a MOF nanozyme model, can catalyze hydrogen peroxide to produce hydroxyl radicals (^•OH), which can efficiently oxidize electroactive probe (such as 1,2-phenylenediamine (o-PD)) accompanying with intense electrochemical signals. Modification of MOF nanozyme on the electrode and capture of antibodies for binding target antigens hinder the catalytic process of MOF nanozyme toward o-PD, resulting in a gradual decrease in electrochemical signal with increasing target antigen concentration, enabling quantitative label-free immunoassay. Thus, a highly sensitive label-free immunosensor using MOF nanozyme-induced catalytic amplification achieved effective detection of Immunoglobulin G (IgG) with a wide linear range of 0.001–50 ng/mL and low detection limit of 0.45 pg/mL. This work proposes a promising nanozyme-induced catalytic amplification strategy for the development of label-free electrochemical immunoassay.
- MOF nanozyme,
- Catalytic amplification,
- POD-like,
- Label-free,
- Electrochemical immunosensor
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