Citation: Ruixin Liu, Feng Shi, Yanping Xia, Haibing Zhu, Jiawen Cao, Kai Peng, Chuanli Ren, Juan Li, Zhanjun Yang. Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay[J]. Chinese Chemical Letters, ;2024, 35(11): 109664. doi: 10.1016/j.cclet.2024.109664 shu

Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay

Ruixin Liu ^{a, 1} ,
Feng Shi ^{a, 1} ,
Yanping Xia ^{a, 1} ,
Haibing Zhu ^a ,
Jiawen Cao ^a ,
Kai Peng ^a ,
Chuanli Ren ^{b, *,} ,
Juan Li ^{a, *,} ,
Zhanjun Yang ^{a, *,}

a.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
b.
Clinical Medical College of Yangzhou University, Northern Jiangsu People’s Hospital Affiliated to Yangzhou University, Yangzhou University, Yangzhou 225001, China

renchl@163.com

lijuan@yzu.edu.cn

zjyang@yzu.edu.cn

Received Date: 28 October 2023
Revised Date: 27 January 2024
Accepted Date: 19 February 2024
Available Online: 28 February 2024

Figures(6)

Label-free immunoassay is confronted with a great challenge that its insufficient sensitivity for low concentration analytes, which can be assigned to the low catalytic efficiency of modified materials towards electroactive molecules. Herein, a universal MOF nanozyme-induced catalytic amplification strategy was proposed for constructing highly sensitive label-free electrochemical immunoassay. Specifically, the synthesized CuFe-MOF nanozyme with superior peroxidase (POD)-like activity, regarding as a MOF nanozyme model, can catalyze hydrogen peroxide to produce hydroxyl radicals (^•OH), which can efficiently oxidize electroactive probe (such as 1,2-phenylenediamine (o-PD)) accompanying with intense electrochemical signals. Modification of MOF nanozyme on the electrode and capture of antibodies for binding target antigens hinder the catalytic process of MOF nanozyme toward o-PD, resulting in a gradual decrease in electrochemical signal with increasing target antigen concentration, enabling quantitative label-free immunoassay. Thus, a highly sensitive label-free immunosensor using MOF nanozyme-induced catalytic amplification achieved effective detection of Immunoglobulin G (IgG) with a wide linear range of 0.001–50 ng/mL and low detection limit of 0.45 pg/mL. This work proposes a promising nanozyme-induced catalytic amplification strategy for the development of label-free electrochemical immunoassay.
- MOF nanozyme,
- Catalytic amplification,
- POD-like,
- Label-free,
- Electrochemical immunosensor
1. [1]
  J.X. Zhang, C.L. Lv, C. Tang, et al., Sens. Actuat. B: Chem. 382 (2023) 133497. doi: 10.1016/j.snb.2023.133497
2. [2]
  T. Dong, N.M.M. Pires, Z.C. Yang, Z.D. Jiang, Adv. Sci. 10 (2023) 2205429. doi: 10.1002/advs.202205429
3. [3]
  C.X. Zhao, Y.J. Li, J.J. Zhao, et al., ACS Nano 17 (2023) 13296–13309. doi: 10.1021/acsnano.3c01158
4. [4]
  Q.C. Lan, H.F. Shen, J. Li, et al., Chem. Communn. 56 (2020) 699–702. doi: 10.1039/C9CC07934C
5. [5]
  Z.J. Yang, Q.C. Lan, J. Li, et al., Biosens. Bioelectron. 89 (2017) 312–318. doi: 10.1016/j.bios.2016.09.026
6. [6]
  Q.C. Lan, C.L. Ren, A. Lambert, et al., ACS Sustain. Chem. Eng. 8 (2020) 4392–4399. doi: 10.1021/acssuschemeng.9b06858
7. [7]
  Y.P. Xia, H. Chen, R.X. Liu, et al., J. Anal. Test. 7 (2023) 295–303. doi: 10.1007/s41664-023-00255-1
8. [8]
  H.H. Rao, X. Xue, M.Y. Luo, H.X. Liu, Z.H. Xue, Chin. Chem. Lett. 32 (2021) 25–32. doi: 10.1016/j.cclet.2020.09.017
9. [9]
  H.N. Chen, Y.Y. Li, Y.C. Song, et al., Biosens. Bioelectron. 223 (2023) 115029. doi: 10.1016/j.bios.2022.115029
10. [10]
  D.S. Zhang, W.X. Li, H.Q. Wang, Z.F. Ma, Sens. Actuat. B: Chem. 258 (2018) 141–147. doi: 10.1016/j.snb.2017.11.085
11. [11]
  Y.F. Dai, L.Y. Chiu, Y.N. Chen, et al., ACS Sens. 4 (2019) 161–169. doi: 10.1021/acssensors.8b01126
12. [12]
  H. Wang, X.L. Wang, R.M. Kong, L. Xia, F.L. Qu, Chin. Chem. Lett. 32 (2021) 198–202. doi: 10.1016/j.cclet.2020.10.017
13. [13]
  J.B. Ouyang, J. Chen, S.Q. Ma, et al., Particuology 62 (2022) 71–78. doi: 10.1016/j.partic.2021.04.012
14. [14]
  Z.H. Li, T.M. Rayder, L.S. Luo, J.A. Byers, C.K. Tsung, J. Am. Chem. Soc. 140 (2018) 8082–8085. doi: 10.1021/jacs.8b04047
15. [15]
  K. Suresh, A.J. Matzger, Angew. Chem. Int. Ed. 58 (2019) 16790–16794. doi: 10.1002/anie.201907652
16. [16]
  X.J. Liu, X. Gao, L.M. Yang, Y.C. Zhao, F. Li, Anal. Chem. 93 (2021) 11792–11799. doi: 10.1021/acs.analchem.1c02286
17. [17]
  J.L. Guo, H.J. Xu, J.J. Zhao, et al., Chem. Sci. 13 (2022) 9993. doi: 10.1039/D2SC03198A
18. [18]
  J.L. Guo, L.L. Yang, Z.D. Gao, et al., ACS Catal. 10 (2020) 5949–5958. doi: 10.1021/acscatal.0c00591
19. [19]
  N. Cheng, C.Z. Zhu, Y.L. Wang, et al., J. Anal. Test. 3 (2019) 99–106. doi: 10.1007/s41664-018-0079-z
20. [20]
  J.L. Guo, X.A. Liu, J.J. Zhao, et al., Chem. Sci. 14 (2023) 1742. doi: 10.1039/D2SC05784K
21. [21]
  T. Hou, N.N. Xu, X. Song, L.M. Yang, F. Li, Chin. Chem. Lett. 34 (2023) 107907. doi: 10.1016/j.cclet.2022.107907
22. [22]
  D.D. Wang, H.H. Wu, W.Q. Lim, et al., Adv. Mater. 31 (2019) 1901893. doi: 10.1002/adma.201901893
23. [23]
  J.Y. Zhao, Y.Y. Long, C.J. He, et al., Chem. Eng. J. 479 (2024) 147513. doi: 10.1016/j.cej.2023.147513
24. [24]
  M. Saeed, L.Y. Deng, Int. J. Greenh. Gas Control 53 (2016) 254–262. doi: 10.1016/j.ijggc.2016.08.017
25. [25]
  X.L. Wang, Z.L. Cheng, Y. Zhou, S.K. Tammina, Y.L. Yang, Microchim. Acta 187 (2020) 350. doi: 10.1007/s00604-020-04322-7
26. [26]
  Y.W. Mao, J.X. Zhang, D.N. Chen, A.J. Wang, Sens. Actuat. B: Chem. 370 (2022) 132416. doi: 10.1016/j.snb.2022.132416
27. [27]
  J.M. Xu, J. Ma, Y. Peng, et al., Chin. Chem. Lett. 34 (2023) 107527. doi: 10.1016/j.cclet.2022.05.041
28. [28]
  J.Y. Zhan, F. Shi, J. Li, et al., Chin. Chem. Lett. 34 (2023) 108791. doi: 10.1016/j.cclet.2023.108791
29. [29]
  J.J. Luo, R.L. Liu, S.C. Zhao, Y. Gao, J. Anal. Test. 7 (2023) 53–68. doi: 10.1007/s41664-022-00241-z
30. [30]
  S.Y. Yin, G.S. Song, Y. Yang, et al., Adv. Funct. Mater. 29 (2019) 1901417. doi: 10.1002/adfm.201901417
31. [31]
  Y.H. Xia, K.M. Sun, Y.N. Zuo, S.Y. Zhu, X.E. Zhao, Chin. Chem. Lett. 33 (2022) 2081–2085. doi: 10.1016/j.cclet.2021.08.083
32. [32]
  C.C. Ma, Y. Guo, H.O. Liu, X.F. Zhang, Micropor. Mesopor. Mater. 322 (2021) 111153. doi: 10.1016/j.micromeso.2021.111153
33. [33]
  J. Zeng, K.C. Devarayapalli, S.V.P. Vattikuti, J. Shim, Mater. Lett. 309 (2022) 131305. doi: 10.1016/j.matlet.2021.131305
34. [34]
  X. Zhang, X.D. Zhang, Y.P. Cai, S.G. Wang, Chem. Eng. J. 443 (2022) 136412. doi: 10.1016/j.cej.2022.136412
35. [35]
  Q.S. Jiang, Y.C. Xiao, A.N. Hong, et al., ACS Appl. Mater. Interfaces 14 (2022) 41800–41808. doi: 10.1021/acsami.2c12115
36. [36]
  P.K. Cao, K. Zhao, X. Quan, S. Chen, H.T. Yu, Sep. Purif. Technol. 238 (2020) 116424. doi: 10.1016/j.seppur.2019.116424
37. [37]
  Y. Qu, L. Chen, H.P. Deng, C.Y. Li, J. Solid State Electr. 21 (2017) 3281–3287. doi: 10.1007/s10008-017-3641-0
38. [38]
  S.C. Barman, M.A. Zahed, M. Sharifuzzaman, et al., Adv. Funct. Mater. 30 (2020) 1907297. doi: 10.1002/adfm.201907297
39. [39]
  G.L. Hong, C.P. Su, Z.N. Huang, et al., Anal. Chem. 93 (2021) 13022–13028. doi: 10.1021/acs.analchem.1c02850
40. [40]
  S. Chanarsa, J.J. akmunee, K. Ounnunkad, Front. Chem. 9 (2021) 631571. doi: 10.3389/fchem.2021.631571
1. [1]
  Yijian Zhao , Jvzhe Li , Yunyi Shi , Jie Hu , Meiyi Liu , Yao Shen , Xinglin Hou , Qiuyue Wang , Qi Wang , Zhiyi Yao . A label-free and ratiometric fluorescent sensor based on porphyrin-metal-organic frameworks for sensitive detection of ochratoxin A in cereal. Chinese Chemical Letters, 2025, 36(4): 110132-. doi: 10.1016/j.cclet.2024.110132
2. [2]
  Xiaoshuai Wu , Bailei Wang , Yichen Li , Xiaoxuan Guan , Mingjing Yin , Wenquan Lv , Yin Chen , Fei Lu , Tao Qin , Huyang Gao , Weiqian Jin , Yifu Huang , Cuiping Li , Ming Gao , Junyu Lu . NIR driven catalytic enhanced acute lung injury therapy by using polydopamine@Co nanozyme via scavenging ROS. Chinese Chemical Letters, 2025, 36(2): 110211-. doi: 10.1016/j.cclet.2024.110211
3. [3]
  Yuwan Lu , Xiaodan Zhang , Yuming Huang . Dual-site Se/NC specific peroxidase-like nanozyme for highly sensitive methimazole detection. Chinese Chemical Letters, 2025, 36(4): 110129-. doi: 10.1016/j.cclet.2024.110129
4. [4]
  Simin Wei , Yaqing Yang , Junjie Li , Jialin Wang , Jinlu Tang , Ningning Wang , Zhaohui Li . The Mn/Yb/Er triple-doped CeO₂ nanozyme with enhanced oxidase-like activity for highly sensitive ratiometric detection of nitrite. Chinese Chemical Letters, 2024, 35(6): 109114-. doi: 10.1016/j.cclet.2023.109114
5. [5]
  Wenhao Feng , Chunli Liu , Zheng Liu , Huan Pang . In-situ growth of N-doped graphene-like carbon/MOF nanocomposites for high-performance supercapacitor. Chinese Chemical Letters, 2024, 35(12): 109552-. doi: 10.1016/j.cclet.2024.109552
6. [6]
  Hai-Yang Song , Jun Jiang , Yu-Hang Song , Min-Hang Zhou , Chao Wu , Xiang Chen , Wei-Min He . Supporting-electrolyte-free electrochemical [2 + 2 + 1] annulation of benzo[d]isothiazole 1,1-dioxides, N-arylglycines and paraformaldehyde. Chinese Chemical Letters, 2024, 35(6): 109246-. doi: 10.1016/j.cclet.2023.109246
7. [7]
  Kexin Yin , Jingren Yang , Yanwei Li , Qian Li , Xing Xu . Metal-free diatomaceous carbon-based catalyst for ultrafast and anti-interference Fenton-like oxidation. Chinese Chemical Letters, 2024, 35(12): 109847-. doi: 10.1016/j.cclet.2024.109847
8. [8]
  Zhaorui Song , Qiulian Hao , Bing Li , Yuwei Yuan , Shanshan Zhang , Yongkuan Suo , Hai-Hao Han , Zhen Cheng . NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers. Chinese Chemical Letters, 2025, 36(1): 109834-. doi: 10.1016/j.cclet.2024.109834
9. [9]
  Erzhuo Cheng , Yunyi Li , Wei Yuan , Wei Gong , Yanjun Cai , Yuan Gu , Yong Jiang , Yu Chen , Jingxi Zhang , Guangquan Mo , Bin Yang . Galvanostatic method assembled ZIFs nanostructure as novel nanozyme for the glucose oxidation and biosensing. Chinese Chemical Letters, 2024, 35(9): 109386-. doi: 10.1016/j.cclet.2023.109386
10. [10]
  Lixian Cai , Yingxiang Ye . A flexible-robust MOF for efficient purification of perfluoropropane. Chinese Journal of Structural Chemistry, 2024, 43(11): 100368-100368. doi: 10.1016/j.cjsc.2024.100368
11. [11]
  Qinghong Pan , Huafang Zhang , Qiaoling Liu , Donghong Huang , Da-Peng Yang , Tianjia Jiang , Shuyang Sun , Xiangrong Chen . A self-powered cathodic molecular imprinting ultrasensitive photoelectrochemical tetracycline sensor via ZnO/C photoanode signal amplification. Chinese Chemical Letters, 2025, 36(1): 110169-. doi: 10.1016/j.cclet.2024.110169
12. [12]
  Bo Liu , Shuaiqiang Shao , Junjie Cai , Zijian Zhang , Feng Tian , Kun Yang , Fan Li . Signal cascade amplification of streptavidin-biotin-modified immunofluorescence nanocapsules for ultrasensitive detection of glial fibrillary acidic protein. Chinese Chemical Letters, 2025, 36(3): 109814-. doi: 10.1016/j.cclet.2024.109814
13. [13]
  Jingting Wang , Yuanyuan Chen , Linlin Han , Shasha Xia , Xingyao Zhang , Peng Xue , Yuejun Kang , Jian Ming , Zhigang Xu . Microenvironment responsive pod-structured astaxanthin nanocarrier for ameliorating inflammatory bowel disease. Chinese Chemical Letters, 2024, 35(7): 109029-. doi: 10.1016/j.cclet.2023.109029
14. [14]
  Ji Liu , Dongsheng He , Tianjiao Hao , Yumin Hu , Yan Zhao , Zhen Li , Chang Liu , Daquan Chen , Qiyue Wang , Xiaofei Xin , Yan Shen . Gold mineralized "hybrid nanozyme bomb" for NIR-II triggered tumor effective permeation and cocktail therapy. Chinese Chemical Letters, 2024, 35(9): 109296-. doi: 10.1016/j.cclet.2023.109296
15. [15]
  Caixia Zhu , Qing Hong , Kaiyuan Wang , Yanfei Shen , Songqin Liu , Yuanjian Zhang . Single nanozyme-based colorimetric biosensor for dopamine with enhanced selectivity via reactivity of oxidation intermediates. Chinese Chemical Letters, 2024, 35(10): 109560-. doi: 10.1016/j.cclet.2024.109560
16. [16]
  Yue Sun , Yingnan Zhu , Jiahang Si , Ruikang Zhang , Yalan Ji , Jinjie Fan , Yuze Dong . Glucose-activated nanozyme hydrogels for microenvironment modulation via cascade reaction in diabetic wound. Chinese Chemical Letters, 2025, 36(4): 110012-. doi: 10.1016/j.cclet.2024.110012
17. [17]
  Jiqing Liu , Qi Dang , Liting Wang , Dejin Wang , Liang Tang . Applications of flexible electrochemical electrodes in wastewater treatment: A review. Chinese Chemical Letters, 2024, 35(8): 109277-. doi: 10.1016/j.cclet.2023.109277
18. [18]
  Yanqi Wu , Yuhong Guan , Peilin Huang , Hui Chen , Liping Bai , Zhihong Jiang . Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform. Chinese Chemical Letters, 2024, 35(9): 109308-. doi: 10.1016/j.cclet.2023.109308
19. [19]
  Sixin Ai , Wenxiu Li , Huayong Zhu , Yang Wan , Weiying Lin . Viscosity-responsive signal amplification dual-modal probe triggered by cysteine/homocysteine for monitoring diabetic liver damages and repair processes. Chinese Chemical Letters, 2025, 36(3): 109904-. doi: 10.1016/j.cclet.2024.109904
20. [20]
  Ming-Yi Sun , Lu Zhang , Ya Li , Chong-Chen Wang , Peng Wang , Xueying Ren , Xiao-Hong Yi . Recovering Ag⁺ with nano-MOF-303 to form Ag/AgCl/MOF-303 photocatalyst: The role of stored Cl⁻ ions. Chinese Chemical Letters, 2025, 36(2): 110035-. doi: 10.1016/j.cclet.2024.110035

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(272)
HTML views(11)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142