Citation: Yanqi Wu, Yuhong Guan, Peilin Huang, Hui Chen, Liping Bai, Zhihong Jiang. Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform[J]. Chinese Chemical Letters, ;2024, 35(9): 109308. doi: 10.1016/j.cclet.2023.109308 shu

Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform

Yanqi Wu ^{a, b, 1} ,
Yuhong Guan ^{c, 1} ,
Peilin Huang ^b ,
Hui Chen ^c ,
Liping Bai ^a ,
Zhihong Jiang ^{a, *,}

a.
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao 999078, China
b.
Shenzhen LemnisCare Medical Technology Co., Ltd., Shenzhen 518107, China
c.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412077, China

zhjiang@must.edu.mo

Received Date: 8 September 2023
Revised Date: 11 November 2023
Accepted Date: 14 November 2023
Available Online: 21 November 2023

Figures(4)

Norovirus is an infectious disease that can cause non-bacterial gastroenteritis, which has a low infectious dose, rapid onset, and strong transmission ability; therefore, rapid and sensitive detection is essential to reduce the transmission of gastroenteritis. In the study, a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres, and a closed-cassette-based, integrated, reagent-ambient storage, on-site instant detection platform for norovirus GII was constructed using a commercial, fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection, with a sensitivity of 10 copies/µL, with no cross-reactivity with other 5 viruses. For 28 simulated samples, the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays after conventional laboratory nucleic acid extraction. The entire process can be finished in about 1 h, which is ideal for immediate rapid detection.
- Freeze-dried microspheres,
- Point-of-care testing,
- Loop-mediated isothermal amplification,
- Norovirus GII,
- Integrated testing
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