Citation: Ke Quan, Jiajie Tong, Lifang Chen, Shuyao Fang, Mengjiao Li, Linlin Wu, Zhihe Qing. Freezing-directed construction of enzyme/nano interfaces: Reagentless conjugation, superior activity, and better stability[J]. Chinese Chemical Letters, ;2024, 35(1): 108894. doi: 10.1016/j.cclet.2023.108894 shu

Freezing-directed construction of enzyme/nano interfaces: Reagentless conjugation, superior activity, and better stability

Ke Quan ^{a, 1} ,
Jiajie Tong ^{a, 1} ,
Lifang Chen ^{a, 1} ,
Shuyao Fang ^a ,
Mengjiao Li ^a ,
Linlin Wu ^b ,
Zhihe Qing ^{a, *,}

a.
Hunan Provincial Key Laboratory of Cytochemistry, School of Food and Bioengineering, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China
b.
Department of Oncology, Tengzhou Central People's Hospital Affiliated Xuzhou Medical University, Tengzhou 277500, China

qingzhihe@hnu.edu.cn

Received Date: 29 March 2023
Revised Date: 17 July 2023
Accepted Date: 4 August 2023
Available Online: 5 August 2023

Figures(5)

Immobilizing enzyme to nano interfaces has demonstrated to be a favorable strategy for prompting the industrialized application of enzyme. Despite tremendous endeavor has been devoted to using gold nanoparticles (AuNPs) as conjugation matrix due to its fascinating physico-chemical properties, maintaining enzymatic activity while circumventing cumbersome modification remains a formidable challenge. Herein, the freezing-directed conjugation of enzyme/nano interfaces was constructed without extra reagent. As the proof of concept, glucose oxidase (GOx) was chosen as model enzyme. The one-pot conjugation process can be facilely completed at −20 ℃ under aqueous solution. Moreover, with the loading of GOx on AuNP at freezing, the enzyme exhibited superior catalytic activity and stability upon thermal and pH perturbation. The mechanism of boosted activity was then discussed in detail. It was found that higher loading density under freezing condition and more enzyme tending to bind AuNPs via Au-S bond were the main factors for the superior activity. More importantly, this methodology was universal and can also be applied to other enzyme which contains natural cysteine, such as horseradish peroxidase (HRP) and papain. This facile conjugation strategy accompanied by remarkable bioactivity expand the possibilities for enzymatic biosensing, microdevice and even drug delivery.
- Enzyme conjugation,
- Freezing,
- AuNPs,
- Catalytic activity
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