Citation: Wangyan Hu, Ke Li, Xiangnan Dou, Ning Li, Xiayan Wang. Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography[J]. Chinese Chemical Letters, ;2024, 35(4): 108806. doi: 10.1016/j.cclet.2023.108806 shu

Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography

Wangyan Hu ^a ,
Ke Li ^b ,
Xiangnan Dou ^{a, *,} ,
Ning Li ^a ,
Xiayan Wang ^{a, *,}

a.
Center Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry, Beijing University of Technology, Beijing 100124, China
b.
Center for Environmental Metrology, National Institute of Metrology, Beijing 100029, China

douxn001@bjut.edu.cn

xiayanwang@bjut.edu.cn

Received Date: 28 March 2023
Revised Date: 21 June 2023
Accepted Date: 13 July 2023
Available Online: 15 July 2023

Figures(5)

Modern chromatography is increasingly focused on miniaturization and integration. Compared to conventional liquid chromatography, microfluidic chip liquid chromatography (microchip-LC) has the potential due to its zero-dead volume connection and ease of integration. Nano-sized packings have the potential to significantly enhance separation performance in microchip-LC. However, their application has been hindered by packing difficulties. This study presents a method for packing nano-sized silica particles into a microchannel as the stationary phase. The microchip-LC packed column was prepared by combining the weir and the porous silica single-particle as frit to retain the packing particles. A surface tension-based single-particle picking technique was established to insert porous single-particle frit into glass microchannels. Additionally, we developed a slurry packing method that utilizes air pressure to inject nano-sized packing into the microchannel. Pressure-driven chromatographic separation was performed using this nano-packed column integrated into a glass microchip. The mixture of four PAHs was successfully separated within just 8 min using a 5 mm separation channel length, achieving high theoretical plates (10⁶ plates/m). Overall, these findings demonstrate the potential of utilizing nano-sized packings for enhancing chromatographic performance in microchip systems.
- Nano-size packing,
- Single-particle frit,
- Packed column,
- Microchip LC,
- Chromatographic separation
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