Citation: Wenling Yuan, Fengli Li, Zhe Chen, Qiaoxin Xu, Zhenhua Guan, Nanyu Yao, Zhengxi Hu, Junjun Liu, Yuan Zhou, Ying Ye, Yonghui Zhang. AbnI: An α-ketoglutarate-dependent dioxygenase involved in brassicicene CH functionalization and ring system rearrangement[J]. Chinese Chemical Letters, ;2024, 35(5): 108788. doi: 10.1016/j.cclet.2023.108788 shu

AbnI: An α-ketoglutarate-dependent dioxygenase involved in brassicicene CH functionalization and ring system rearrangement

Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

ying_ye@hust.edu.cn

zhangyh@mails.tjmu.edu.cn

Received Date: 24 May 2023
Revised Date: 3 July 2023
Accepted Date: 5 July 2023
Available Online: 7 July 2023

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We reported the characterization of a novel brassicicene diterpene biosynthetic gene cluster, which contains a unique α-ketoglutarate-dependent dioxygenase (αKGD) enzyme, AbnI. Our findings revealed that AbnI demonstrates remarkable substrate promiscuity and is capable of activating multiple sites on both 5–8–5 and 5–9–5 brassicicene skeletons, resulting in skeleton modifications and an unexpected ring system rearrangement. These results suggested the potential utility of AbnI as an enzymatic tool for terpene CH functionalization. In addition, the catalytic mechanism of AbnI and its potential ecological implications were discussed.
- Fungal diterpenes,
- α-Ketoglutarate-dependent dioxygenase,
- C-H functionalization,
- Rearrangement,
- Brassicicenes
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