Citation: Binglu Zhao, Mashooq Khan, Yulin Liu, Wenjun Tai, Chongyang Mu, Wenli Wu, Mei Zhao, Yaohong Ma, Li Yu, Jin-Ming Lin, Qiongzheng Hu. Distance-based α-amylase biosensor fabricated with amylopectin-coated mesoporous membrane[J]. Chinese Chemical Letters, ;2024, 35(1): 108462. doi: 10.1016/j.cclet.2023.108462 shu

Distance-based α-amylase biosensor fabricated with amylopectin-coated mesoporous membrane

Binglu Zhao ^{a, b} ,
Mashooq Khan ^{a, b} ,
Yulin Liu ^a ,
Wenjun Tai ^c ,
Chongyang Mu ^c ,
Wenli Wu ^{a, b} ,
Mei Zhao ^{a, b} ,
Yaohong Ma ^d ,
Li Yu ^c ,
Jin-Ming Lin ^e ,
Qiongzheng Hu ^{a, b, *,}

a.
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Ji'nan 250014, China
b.
School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250014, China
c.
Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Ji'nan 250100, China
d.
Key Laboratory for Biosensors of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250353, China
e.
Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China

huqz@qlu.edu.cn

Received Date: 10 February 2023
Revised Date: 22 March 2023
Accepted Date: 14 April 2023
Available Online: 16 April 2023

Figures(5)

Paper-based biosensors are widely employed in point-of-care testing (POCT) due to their convenience, portability, low cost, and ease of use. This study reports an integrated distance-based paper biosensor fabricated with a mesoporous membrane coated with stimuli-responsive polymer. The detection of α-amylase (AMY) using amylopectin-coated mesoporous membrane is demonstrated as an example. After introducing the AMY solution, it is observed that the aqueous solution flows along the paper strip due to AMY-catalyzed hydrolysis of amylopectin. The flow distance is proportional to the concentration of AMY with a detection limit as low as 4 mU/mL. In addition, the detection of AMY is demonstrated in human serum. Furthermore, the inhibitory effect of acarbose on AMY is evaluated. This reagent-free and disposable biosensor allows single-step rapid detection of the analyte. This approach is very promising for the development of user-friendly, equipment-free, and cost-effective biosensors with remarkable sensitivity and excellent selectivity for disease diagnosis and hypoglycemic drug screening.
- Paper biosensor,
- α-Amylase,
- Stimuli-responsive polymer,
- Mesoporous membrane,
- Distance,
- Point-of-care testing
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