Citation: Haocheng Gong, Yanhui Zhang, Ying Gao, Xinrong Tian, Pengzhao Wu, Xiaoyu Wei, Yuan Guo. In vivo precision imaging of vicinal-dithiol-containing proteins by a FRET molecular probe sensitive to protein environment[J]. Chinese Chemical Letters, ;2023, 34(12): 108329. doi: 10.1016/j.cclet.2023.108329 shu

In vivo precision imaging of vicinal-dithiol-containing proteins by a FRET molecular probe sensitive to protein environment

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710127, China

guoyuan@nwu.edu.cn

Received Date: 11 December 2022
Revised Date: 9 March 2023
Accepted Date: 10 March 2023
Available Online: 13 March 2023

Figures(4)

We here present a Förster resonance energy transfer (FRET)-based and environment-sensitive fluorescent probe VG-1 for vicinal-dithiol-containing proteins (VDPs). VG-1 uniquely contains two sites sensitive to the protein environment (SPE), thus it shows weak fluorescence in both blue and green channels (a low FRET efficiency) in solution. After specifically binding with VDPs, its fluorescence in the green channel increases, while that in the blue channel disappears, achieving the specific detection of VDPs. The obvious signal changes in fluorescence may be attributed to that the increased rigidity of the molecular skeletons causes the enhanced FRET efficiency. The probe also achieved the cell super-resolution imaging of VDPs and the confocal imaging of VDPs in zebrafish.
- Vicinal-dithiol-containing proteins,
- Environment sensitivity,
- Super-resolution imaging,
- Fluorescent probes,
- Förster resonance energy transfer
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沈阳化工大学材料科学与工程学院沈阳 110142