Citation: Zhen Song, Rong Li, Xiqin Yang, Adriano Ambrosi, Xiliang Luo. Ultralow fouling electrochemical detection of uric acid directly in serum based on phase-transited bovine serum albumin and conducting polymer[J]. Chinese Chemical Letters, ;2023, 34(12): 108314. doi: 10.1016/j.cclet.2023.108314 shu

Ultralow fouling electrochemical detection of uric acid directly in serum based on phase-transited bovine serum albumin and conducting polymer

Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

xiliangluo@qust.edu.cn

Received Date: 7 November 2022
Revised Date: 14 February 2023
Accepted Date: 7 March 2023
Available Online: 11 March 2023

Figures(7)

Electrochemistry with antifouling sensing interfaces that effectively resist the adsorption of nonspecific biomolecules provides a powerful mean for the accurate and sensitive detection of disease biomarkers in complex biofluids. However, there are few strategies to acquire a stable and solid antifouling coating on any substrate by a simple way. Herein, a simple one-step assembly method has been adopted to construct phase-transited bovine serum albumin (PTB) antifouling layers. Prior to construction of the antifouling layers, the poly(3,4-ethylenedioxythiophene) (PEDOT) doped with 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (ionic liquid, IL) were firstly electrodeposited on bare electrodes, endowing good conductivity and catalytic capability for the developed sensor. Subsequently, with the assist of tris(2-carboxyethyl)phosphine (TCEP), the disulfide bonds of bovine serum albumin (BSA) were reduced to form PTB, which can be coated on the PEDOT-IL modified electrode to construct an antifouling electrochemical senor (PTB/PEDOT-IL/GCE) for the detection of uric acid (UA) in human serum. The UA sensor demonstrated a good linear range from 1.11 µmol/L to 798.9 µmol/L, with a high sensitivity of 0.556 µA µmol L⁻¹ cm⁻². The combination of conducting polymers with one-step assembly of PTB offers a universal and reliable method for the modification of various electrodes to determine target molecules in complex human body fluids.
- Phase-transited bovine serum albumin,
- Antifouling,
- Electrochemical sensor,
- Uric acid,
- Conducting polymer
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