Citation: Xiang Sun, Yanmin Wang, Tao Du, Qiang Zhang, Shuo Li, Qinda Chen, Miao Wang, Xiumin Wang, Lei Ren, Xueqin Zhao. Indocyanine green-/TLR7 agonist-constructed thermosensitive liposome for low-temperature PTT induced synergistic immunotherapy of colorectal cancer[J]. Chinese Chemical Letters, ;2023, 34(5): 108201. doi: 10.1016/j.cclet.2023.108201 shu

Indocyanine green-/TLR7 agonist-constructed thermosensitive liposome for low-temperature PTT induced synergistic immunotherapy of colorectal cancer

Xiang Sun ^a ,
Yanmin Wang ^a ,
Tao Du ^a ,
Qiang Zhang ^a ,
Shuo Li ^b ,
Qinda Chen ^c ,
Miao Wang ^{a, *,} ,
Xiumin Wang ^c ,
Lei Ren ^{a, d, *,} ,
Xueqin Zhao ^{b, *,}

a.
Department of Biomaterials, Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, College of Materials, Xiamen University, Xiamen 361005, China
b.
Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
c.
School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China
d.
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

miaowang@xmu.edu.cn

renlei@xmu.edu.cn

zhaoxueqin@zstu.edu.cn

Received Date: 4 October 2022
Revised Date: 1 February 2023
Accepted Date: 8 February 2023
Available Online: 10 February 2023

Figures(4)

Colorectal cancer (CRC) is a lethal malignancy with a high mortality rate due to its low immunogenicity, the strong immunosuppressive milieu and poor drug permeability. To overcome these obstacles, a cascade synergistic nanosystem (denoted as R837/ICG@Lip) was developed via self-assembly of heater indocyanine green (ICG) and toll-like receptor-7 agonist imiquimod (R837) into thermosensitive liposome for simultaneous induction of immunogenic cell death (ICD) and reversing of suppressive tumor microenvironment. The obtained nanoparticles exhibited NIR-triggered drug release, good photothermal conversion efficiency and phototoxicity towards CT26 colorectal cancer cells. In vivo results reveal that the R837/ICG@Lip could be effectively accumulated in CT26 subcutaneous tumors and the draining lymph nodes. More importantly, R837/ICG@Lip-mediated low-temperature photothermal therapy triggers ICD, promotes the maturation of host dendritic cells (DCs), and subsequently amplifies adaptive antitumor T-cell responses, resulting in 'Cold to Hot' transition. Besides directly affecting immune cells, the secretion of some immune-related cytokines further indirectly boosted anti-cancer immunity. After combining with the indoleamine 2, 3-dioxygenase (IDO) inhibitor, the systemic antitumor immune response was further augmented, achieving best tumor inhibition effects. Thus, low-temperature mediated photoimmunotherapy targeting multiple antitumor immune pathways boost synergistic antitumor immunity of tolerance tumors.
- Colorectal cancer,
- Liposomes,
- Photothermal therapy,
- Immunotherapy,
- Indocyanine green
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