Citation: Xiaorui Wang, Gaowa Xing, Nan Li, Yaoshuang Xie, Ling Lin. An integrated microfluidic device for the simultaneous detection of multiple antibiotics[J]. Chinese Chemical Letters, ;2023, 34(8): 108110. doi: 10.1016/j.cclet.2022.108110 shu

An integrated microfluidic device for the simultaneous detection of multiple antibiotics

Xiaorui Wang ^a ,
Gaowa Xing ^b ,
Nan Li ^c ,
Yaoshuang Xie ^a ,
Ling Lin ^{a, *,}

a.
Department of Bioengineering, Beijing Technology and Business University, Beijing 100048, China
b.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China
c.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China

linling@btbu.edu.cn

Received Date: 11 October 2022
Revised Date: 13 December 2022
Accepted Date: 27 December 2022
Available Online: 29 December 2022

Figures(4)

Residual antibiotics in food pose a serious long-term threat to human health. Therefore, an on-site visualization method for antibiotic detection is required. However, the requirements of traditional antibiotic testing methods in terms of operator proficiency and equipment cost hinder the rapid point-of-care-testing detection of suspected samples. Herein, we reported an integrated microfluidic device combining a microfluidic chip containing cruciform valves with immunochromatographic strips for the rapid detection of multiple antibiotics in milk. The rapid qualitative and quantitative analysis of four types of antibiotics (sulfonamides, β-lactams, streptomycin, and tetracyclines) was performed using mobile phone photography and mobile phone application analysis. The detection time was maintained at 10 min. The limits of detection (LODs) for the four antibiotics were 0.15, 0.12, 0.25, and 0.29 ng/mL, respectively, and the selectivity for the different antibiotics was observed even in a highly complex matrix. This device successfully integrated separation and real-time detection onto a chip and might provide a promising perspective for the detection of multiple antibiotics in milk.
- Residual antibiotics,
- Microfluidic chip,
- Cruciform valves,
- Point-of-care-testing (POCT),
- Simultaneous detection
1. [1]
  D. Bitas, A. Kabir, M. Locatelli, V. Samanidou, Separations 5 (2018) 31. doi: 10.3390/separations5020031
2. [2]
  A. Boobis, C. Cerniglia, A. Chicoine, V. Samanidou, Crit. Rev. Toxicol. 47 (2017) 889-903. doi: 10.1080/10408444.2017.1340259
3. [3]
  Q. Wang, Q. Xue, T. Chen, et al., Chin. Chem. Lett. 32 (2021) 609-619. doi: 10.1016/j.cclet.2020.10.025
4. [4]
  L. Maier, C.V. Goemans, J. Wirbel, et al., Nature 599 (2021) 120-124. doi: 10.1038/s41586-021-03986-2
5. [5]
  T. Ramatla, L. Ngoma, M. Adetunji, M. Mwanza, Antibiotics 6 (2017) 34. doi: 10.3390/antibiotics6040034
6. [6]
  Z. Aversa, E.J. Atkinson, M.J. Schafer, et al., Mayo Clin. Proc. 96 (2021) 66-77. doi: 10.1016/j.mayocp.2020.07.019
7. [7]
  A. Aidara-Kane, F.J. Angulo, J.M. Conly, et al., Antimicrob. Resist. Infect. Cont. 7 (2018) 7. doi: 10.1186/s13756-017-0294-9
8. [8]
  M. Chen, R. Luo, S. Li, et al., Anal. Chem. 92 (2020) 13336-13342. doi: 10.1021/acs.analchem.0c02642
9. [9]
  R. De O. Silva, M.G.G. De Menezes, R.C. De Castro, et al., Food Chem. 297 (2019) 124934. doi: 10.1016/j.foodchem.2019.06.001
10. [10]
  M. Han, L. Gong, J. Wang, et al., Sens. Actuators B: Chem. 292 (2019) 94-104. doi: 10.1016/j.snb.2019.04.019
11. [11]
  T. Li, C. Wang, Z. Xu, A. Chakraborty, Chemosphere 254 (2020) 126765. doi: 10.1016/j.chemosphere.2020.126765
12. [12]
  Q. Shi, J. Huang, Y. Sun, et al., Spectrochim. Acta A 197 (2018) 107-113. doi: 10.1016/j.saa.2017.11.045
13. [13]
  B. Al Mughairy, H.A.J. Al-Lawati, Trends Anal. Chem. 124 (2020) 115802. doi: 10.1016/j.trac.2019.115802
14. [14]
  M. Pucetaite, P. Ohlsson, P. Persson, E. Hammer, Soil. Biol. Biochem. 153 (2021) 108078. doi: 10.1016/j.soilbio.2020.108078
15. [15]
  Z. Wu, L. Lin, Chin. Chem. Lett. 33 (2022) 1752-1756. doi: 10.1016/j.cclet.2021.08.100
16. [16]
  J.M.D. Machado, R.R.G. Soares, V. Chu, J.P. Conde, Biosens. Bioelectron. 99 (2018) 40-46. doi: 10.1016/j.bios.2017.07.032
17. [17]
  H.K. Li, H.L. Ye, X.X. Zhao, et al., Chin. Chem. Lett. 32 (2021) 2851-2855. doi: 10.1016/j.cclet.2021.02.042
18. [18]
  W. Qi, L. Zheng, Y. Hou, et al., Food Chem. 381 (2022) 131801. doi: 10.1016/j.foodchem.2021.131801
19. [19]
  G. Xing, W. Zhang, N. Li, et al., Chin. Chem. Lett. 33 (2022) 1743-1751. doi: 10.1016/j.cclet.2021.08.073
20. [20]
  M. Zhang, J. He, Y. Chen, et al., Chin. Chem. Lett. 31 (2020) 2721-2724. doi: 10.1016/j.cclet.2020.05.001
21. [21]
  Q. Zhang, S. Feng, L. Lin, S. Mao, J.M. Lin, Chem. Soc. Rev. 50 (2021) 5333-5348. doi: 10.1039/D0CS01516D
22. [22]
  W. Zhang, N. Li, L. Lin, et al., Small 16 (2020) 1903402. doi: 10.1002/smll.201903402
23. [23]
  L. Lin, L. Yi, F. Zhao, et al., Chem. Sci. 11 (2020) 2744-2749. doi: 10.1039/C9SC06185A
24. [24]
  Y. Zheng, Z. Wu, J.M. Lin, L. Lin, Chin. Chem. Lett. 31 (2020) 451-454. doi: 10.1016/j.cclet.2019.07.036
25. [25]
  Z. Su, W. Hu, L. Ye, D. Gao, J.M. Lin, Chin. Chem. Lett. 34 (2023) 107790. doi: 10.1016/j.cclet.2022.107790
26. [26]
  L. Huang, E. Su, Y. Liu, et al., Chin. Chem. Lett. 32 (2021) 1555-1558. doi: 10.1016/j.cclet.2020.09.055
27. [27]
  Y. Shi, M. Hu, Y. Xing, Y. Li, Mater. Des. 185 (2020) 108219. doi: 10.1016/j.matdes.2019.108219
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4. [4]
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