Citation: Dongxue Zhang, Qin Qin, Liang Qiao. Mass spectrometry profiling of single bacterial cells reveals metabolic regulation during antibiotics induced bacterial filamentation[J]. Chinese Chemical Letters, ;2023, 34(6): 107938. doi: 10.1016/j.cclet.2022.107938 shu

Mass spectrometry profiling of single bacterial cells reveals metabolic regulation during antibiotics induced bacterial filamentation

Dongxue Zhang ^a ,
Qin Qin ^b ,
Liang Qiao ^{a, *,}

a.
Department of Chemistry, Institutes of Biomedical Sciences, and Shanghai Stomatological Hospital, Fudan University, Shanghai 200000, China
b.
Changhai Hospital, The Naval Military Medical University, Shanghai 200433, China

liang_qiao@fudan.edu.cn

Received Date: 2 July 2022
Revised Date: 8 October 2022
Accepted Date: 20 October 2022
Available Online: 23 October 2022

Figures(5)

Bacterial antimicrobial resistance (AMR) is a severe threat to global health and development. Under the stimulation of antibiotics, bacterial cells can undergo filamentation and generate daughter cells with stronger AMR. The current research on bacterial AMR mechanism is mainly conducted with a population of cells. However, bacterial cells exhibit heteroresistance, making the study at population level not reliable. Herein, we developed single bacterial cell metabolic profiling by mass spectrometry (MS) to study bacterial AMR at single-cell level. By utilizing a microprobe controlled by a microoperation platform, single filamentous extended spectrum beta-lactamase (ESBL) producing Escherichia coli (ESBL-E. coli) cells generated by ceftriaxone sodium stimulation can be extracted and spray-ionized for MS analysis. Heterogeneous among ESBL-E. coli cells under the same antibiotic stimulus condition was observed from mass spectra as well as cell morphology. The metabolic profiles by MS of different individual cells can be clustered into subgroups well in accordance with bacterial cell length. Metabolic pathways including arginine and proline metabolism, as well as cysteine and methionine metabolism were disclosed to play an important role in the bacterial SOS-associated filamentation against antibiotics. The microprobe electrospray ionization-MS-based single bacterial cell analysis method is promising in the study of various bacterial AMR mechanism and can reveal the heterogeneity of bacterial AMR from-cell-to-cell.
- ESI-MS,
- Single bacterial cell analysis,
- Metabolome,
- Bacterial antimicrobial resistance,
- Bacterial filamentation
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