Citation: Ye Yang, Mingchan Liang, Rui Wang, Chunmao He. Chemical protein synthesis elucidates key modulation mechanism of the tyrosine-O-sulfation in inducing strengthened inhibitory activity of hirudin[J]. Chinese Chemical Letters, ;2023, 34(5): 107806. doi: 10.1016/j.cclet.2022.107806 shu

Chemical protein synthesis elucidates key modulation mechanism of the tyrosine-O-sulfation in inducing strengthened inhibitory activity of hirudin

Ye Yang ^a ,
Mingchan Liang ^b ,
Rui Wang ^{b, *,} ,
Chunmao He ^{a, *,}

a.
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China
b.
Pingshan translational medicine center, Shenzhen Bay Laboratory, Shenzhen 518118, China

wangrui@szbl.ac.cn

hecm@scut.edu.cn

Received Date: 11 July 2022
Revised Date: 30 August 2022
Accepted Date: 2 September 2022
Available Online: 6 September 2022

Figures(4)

Tyrosine sulfation is an important post-translational modification that enhances the inhibitory activity of hirudin. Herein, we developed a facile synthetic strategy to afford the sulfated hirudins with up to three modifications and in multi-milligram scales, after a single HPLC purification step. Through these synthetic proteins, a novel type of modulation mechanism exhibited by tyrosine sulfation was proposed, which would help to delineate the structure–function relationships in other sulfated proteins and more importantly, to serve as a basis for the development of related antithrombotic agents.
- Tyrosine sulfation,
- Hirudin,
- Chemical protein synthesis,
- Post-translational modification,
- Native chemical ligation
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