Citation: Zhaochen Su, Wanting Hu, Lizhen Ye, Dan Gao, Jin-Ming Lin. An integrated microfluidic chip-mass spectrometry system for rapid antimicrobial resistance analysis of bacteria producing β-lactamases[J]. Chinese Chemical Letters, ;2023, 34(5): 107790. doi: 10.1016/j.cclet.2022.107790 shu

An integrated microfluidic chip-mass spectrometry system for rapid antimicrobial resistance analysis of bacteria producing β-lactamases

Zhaochen Su ^{a, 1} ,
Wanting Hu ^{a, 1} ,
Lizhen Ye ^a ,
Dan Gao ^{a, *,} ,
Jin-Ming Lin ^{b, *,}

a.
State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
b.
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China

gao.dan@sz.tsinghua.edu.cn

jmlin@mail.tsinghua.edu.cn

Received Date: 4 June 2022
Revised Date: 7 August 2022
Accepted Date: 25 August 2022
Available Online: 28 August 2022

Figures(4)

Bacteria producing β-lactamases have become a major issue in the global public health field. To restrain the development of drug resistance and reduce the abuse of antibiotics, it is very important to rapidly identify bacteria producing β-lactamases and put forward a reasonable treatment plan. Here, an integrated microfluidic chip-mass spectrometry system was proposed for rapid screening of β-lactamase-producing bacteria and optimization of β-lactamase inhibitor dosing concentration. The concentration gradient generator followed by an array of bacterial culture chambers, as well as micro-solid-phase extraction columns was designed for sample pretreatment before mass analysis. By using the combination system, the process of the hydrolysis of antibiotics by β-lactamase-producing bacteria could be analyzed. To validate the feasibility, four antibiotics and two antibiotic inhibitors were investigated using three strains including negative control, SHV-1 and TEM-1 strains. SHV-1 and TEM-1 strains were successfully distinguished as the β-lactamase producing strains. And the acquired optimal concentrations of β-lactamase inhibitors were in accordance with the results by that obtained from the traditional microdilution broth method. The total analysis time only needed around 2 h, which was faster than conventional methods that require a few days. The technique presented herein provides an easy and rapid protocol for β-lactamase resistance related studies, which is important for the inhibition of antimicrobial resistance development and the reduction of antibiotics abuse.
- Microfluidics,
- Mass spectrometry,
- β-Lactamases,
- Antimicrobial resistance,
- Inhibitor
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