Citation: Xiao-Hong Wang, Yu Liu, Bin Kang, Jing-Juan Xu, Hong-Yuan Chen. Cell mechanics and energetic costs of collective cell migration under confined microchannels[J]. Chinese Chemical Letters, ;2023, 34(5): 107789. doi: 10.1016/j.cclet.2022.107789 shu

Cell mechanics and energetic costs of collective cell migration under confined microchannels

Xiao-Hong Wang ^{a, b} ,
Yu Liu ^a ,
Bin Kang ^{a, *,} ,
Jing-Juan Xu ^{a, *,} ,
Hong-Yuan Chen ^a

a.
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China
b.
Department of Chemical and Chemical Engineering, Hefei Normal University, Hefei 230601, China

binkang@nju.edu.cn

xujj@nju.edu.cn

Received Date: 7 May 2022
Revised Date: 9 August 2022
Accepted Date: 24 August 2022
Available Online: 28 August 2022

Figures(4)

Mechanical force between cells relates to many biological processes of cell development. The cellular collective migration comes from cell-cell cooperation, and studying the intercellular mechanical properties helps elucidate collective cell migration. Herein, we studied cell-cell junctions, intercellular tensile force and the related cellular energetic costs in confined microchannels. Using the intercellular force sensor, we found that cells adapt to different confinement environments by regulating intercellular force, and thereby the relationship between collective cell migration and cell-cell junction were verified. Through the observation of cell orientation, actomyosin contractility, energetic costs, and glucose uptake, we can make a reasonable explanation of cell-force driven migration in different confined environments. Under highly confined conditions, the intercellular force and energetic costs are greater, and the cell orientation is more orderly. The collective migration behavior in confined spaces is closely related to the intercellular force and energetic costs, which is helpful to understand the collective migration behaviors in various confined spaces.
- Cell mechanics,
- Collective cell migration,
- Cellular energetic costs,
- Fluorescence probe,
- Glucose uptake
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