Citation: Hui Chen, Xinye Ma, Xinyu Zhang, Gui Hu, Yan Deng, Song Li, Zhu Chen, Nongyue He, Yanqi Wu, Zhihong Jiang. Novel aerosol detection platform for SARS-CoV-2: Based on specific magnetic nanoparticles adsorption sampling and digital droplet PCR detection[J]. Chinese Chemical Letters, ;2023, 34(1): 107701. doi: 10.1016/j.cclet.2022.07.044 shu

Novel aerosol detection platform for SARS-CoV-2: Based on specific magnetic nanoparticles adsorption sampling and digital droplet PCR detection

Hui Chen ^{a, *,} ,
Xinye Ma ^a ,
Xinyu Zhang ^a ,
Gui Hu ^a ,
Yan Deng ^a ,
Song Li ^a ,
Zhu Chen ^a ,
Nongyue He ^{a, c, *,} ,
Yanqi Wu ^{b, *,} ,
Zhihong Jiang ^{b, *,}

a.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
b.
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China
c.
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

Received Date: 13 April 2022
Revised Date: 19 July 2022
Accepted Date: 21 July 2022
Available Online: 23 July 2022

Figures(4)

The SARS-CoV-2 virus is released from an infectious source (such as a sick person) and adsorbed on aerosols, which can form pathogenic microorganism aerosols, which can affect human health through airborne transmission. Efficient sampling and accurate detection of microorganisms in aerosols are the premise and basis for studying their properties and evaluating their hazard. In this study, we built a set of sub-micron aerosol detection platform, and carried out a simulation experiment on the SARS-CoV-2 aerosol in the air by wet-wall cyclone combined with immunomagnetic nanoparticle adsorption sampling and ddPCR. The feasibility of the system in aerosol detection was verified, and the influencing factors in the detection process were experimentally tested. As a result, the sampling efficiency was 29.77%, and extraction efficiency was 98.57%. The minimum detection limit per unit volume of aerosols was 250 copies (10² copies/mL, concentration factor 2.5).
- Aerosol detection,
- Air sampling,
- Immunomagnetic nanoparticle,
- ddPCR,
- SARS-CoV-2
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