Citation: Maonan Wang, Zengchao Guo, Jiayu Zeng, Liu Liu, Yihan Wang, Jinpeng Wang, Hongbing Lu, Haijun Zhang, Hui Jiang, Xuemei Wang. Bio-assembled smart nanocapsules for targeted delivery of KRAS shRNA and cancer cell bioimage[J]. Chinese Chemical Letters, ;2023, 34(1): 107651. doi: 10.1016/j.cclet.2022.06.074 shu

Bio-assembled smart nanocapsules for targeted delivery of KRAS shRNA and cancer cell bioimage

Maonan Wang ^a ,
Zengchao Guo ^a ,
Jiayu Zeng ^a ,
Liu Liu ^a ,
Yihan Wang ^a ,
Jinpeng Wang ^a ,
Hongbing Lu ^b ,
Haijun Zhang ^c ,
Hui Jiang ^a ,
Xuemei Wang ^{a, *,}

a.
State Key Laboratory of Bioelectronics (Chien-Shiung Wu Lab), School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Department of Biomedical Engineering/Computer Application, Fourth Military Medical University, Xi'an 710032, China
c.
Department of Oncology, Zhongda Hospital, Medical School of Southeast University, Nanjing 210009, China

xuewang@seu.edu.cn

Received Date: 26 December 2021
Revised Date: 25 June 2022
Accepted Date: 27 June 2022
Available Online: 1 July 2022

Figures(2)

The five-year survival rate for pancreatic cancer is less than 5%. However, the current clinical multimodal therapy combined with first-line chemotherapy drugs only increases the patient's median survival from 5.0 months to 7.2 months. Consequently, a new strategy of cancer treatments is urgently needed to overcome this high-fatality disease. Through a series of biometric analyses, we found that KRAS is highly expressed in the tumor of pancreatic cancer patients, and this high expression is closely related to the poor prognosis of patients. It shows that inhibiting the expression of KRAS has great potential in gene therapy for pancreatic cancer. Given those above, we have exploited the possibility of targeted delivery of KRAS shRNA with the intelligent and bio-responsive nanomedicine to detect the special oxidative stress microenvironment of cancer cells and realize efficient cancer theranostics. Our observations demonstrate that by designing the smart self-assembled nanocapsules of melanin with fluorescent nanoclusters we can readily achieve the bio-recognition and bioimaging of cancer cells in biological solution or serum. The self-assembled nanocapsules can make a significant bio-response to the oxidative stress microenvironment of cancer cells and generate fluorescent zinc oxide Nanoclusters in situ for targeted cell bioimaging. Moreover, it can also readily facilitate cancer cell suppression through the targeted delivery of KRAS shRNA and low-temperature hyperthermia. This raises the possibility to provide a promising theranostics platform and self-assembled nanomedicine for targeted cancer diagnostics and treatments through special oxidative stress-responsive effects of cancer cells.
- Smart bio-responsive nanocapsules,
- Intelligent nanomedicine,
- shRNA,
- KRAS,
- Fluorescent bioimaging,
- Oxidative stress response
1. [1]
  F. Bray, J. Ferlay, I. Soerjomataram, et al., CA Cancer J. Clin. 68 (2018) 394–424. doi: 10.3322/caac.21492
2. [2]
  S. Sun, Y. Liu, C. He, et al., Cancer Lett. 502 (2021) 9–24. doi: 10.1016/j.canlet.2020.12.018
3. [3]
  J.F. Zhang, H.Z. Wang, Pancreas 49 (2020) 1440-1440.
4. [4]
  J. Huang, V. Lok, C. Ngai, et al., Trends in Pancreatic Cancer 160 (2021) 744–754.
5. [5]
  F. Maire, S. Micard, P. Hammel, et al., Brit. J. Cancer 87 (2002) 551–554. doi: 10.1038/sj.bjc.6600475
6. [6]
  S. Li, A. Balmain, C.M. Counter, Nat. Rev. Cancer 18 (2018) 767–777. doi: 10.1038/s41568-018-0076-6
7. [7]
  B. Doiron, W. Hu, L. Norton, R.A. DeFronzo, Diabetologia 55 (2012) 719–728. doi: 10.1007/s00125-011-2414-z
8. [8]
  T. Kokuryo, S. Hibino, K. Suzuki, et al., Cancer Sci. 107 (2016) 1315–1320. doi: 10.1111/cas.12993
9. [9]
  M. Wang, Y. Chen, W. Cai, et al., Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 308. doi: 10.1073/pnas.1915512116
10. [10]
  Y. Lei, L. Tang, Y. Xie, et al., Nat. Commun. 8 (2017) 15130. doi: 10.1038/ncomms15130
11. [11]
  Q. Fan, K. Cheng, X. Hu, et al., J. Am. Chem. Soc. 136 (2014) 15185–15194. doi: 10.1021/ja505412p
12. [12]
  X. Wang, J. Sheng, M. Yang, Chin. Chem. Lett. 30 (2019) 533–540. doi: 10.1016/j.cclet.2018.10.010
13. [13]
  Tayyaba, F. Rehman, S. Shaikh, et al., J. Mater. Chem. B 8 (2020) 2845–2855. doi: 10.1039/c9tb02610j
14. [14]
  Y. Wang, H. Feng, H. Zhang, et al., Analyst 145 (2020) 1294–1301. doi: 10.1039/C9AN02390A
15. [15]
  R.J.C. Slebos, J.A. Hoppin, P.E. Tolbert, et al., Cancer Epidemiol. Biomarkers Prev. 9 (2000) 1223.
16. [16]
  H.H. Loong, N. Du, C. Cheng, et al., Transl. Lung Cancer Res. 9 (2020) 1759–1769. doi: 10.21037/tlcr-20-455
17. [17]
  R. Salgia, R. Pharaon, I. Mambetsariev, A. Nam, M. Sattler, Cell Reports Medicine 2 (2021) 100186. doi: 10.1016/j.xcrm.2020.100186
18. [18]
  H. Ijichi, A. Chytil, A.E. Gorska, et al., Genes Dev. 20 (2006) 3147–3160. doi: 10.1101/gad.1475506
19. [19]
  J. Gout, R.M. Pommier, D.F. Vincent, et al., Pancreatology 13 (2013) 191–195. doi: 10.1016/j.pan.2013.02.001
20. [20]
  J. Lin, M. Wang, H. Hu, et al., Adv. Mater. 28 (2016) 3273–3279. doi: 10.1002/adma.201505700
21. [21]
  K. Morikawa, Y. Masubuchi, Y. Shchipunov, A. Zinchenko, ACS Appl. Bio Mater. 4 (2021) 1823–1832. doi: 10.1021/acsabm.0c01533
22. [22]
  T. Tran, N. Amalina, W. Cheow, K. Hadinoto, J. Drug Deliv. Sci. Tec. 59 (2020) 101866. doi: 10.1016/j.jddst.2020.101866
23. [23]
  D. Wang, Y. Xin, Y. Wang, et al., Chem. Eng. J. 409 (2021) 128082. doi: 10.1016/j.cej.2020.128082
24. [24]
  D. Wang, Y. Xin, X. Li, et al., Chem. Eng. J. 416 (2021) 127625. doi: 10.1016/j.cej.2020.127625
25. [25]
  Z. Hu, S. Wang, Z. Dai, H. Zhang, X. Zheng, J. Mater. Chem. B 8 (2020) 5351–5360. doi: 10.1039/d0tb00708k
26. [26]
  H. Cho, H. Yoon, H. Koo, et al., Biomaterials 32 (2011) 7181–7190. doi: 10.1016/j.biomaterials.2011.06.028
27. [27]
  G. Nabil, R. Alzhrani, H. Alsaab, et al., Cancers (Basel) 13 (2021) 898. doi: 10.3390/cancers13040898
28. [28]
  R.H. Ateya, A.S. Sultan, Cancer Res. 81 (Suppl. 4) (2021) PS19–26.
29. [29]
  G. Gao, Y. Jiang, Y. Guo, et al., Adv. Funct. Mater. 30 (2020) 1909391. doi: 10.1002/adfm.201909391
30. [30]
  T. Du, C. Zhao, F. Rehman, et al., Adv. Funct. Mater. 27 (2016) 1603926.
31. [31]
  L. Lai, C. Zhao, M. Su, et al., Biomater. Sci. 4 (2016) 1085–1091. doi: 10.1039/C6BM00233A
32. [32]
  S. Shaikh, M. Younis, F. Rehman, H. Jiang, X. Wang, Langmuir 36 (2020) 9472–9480. doi: 10.1021/acs.langmuir.0c01378
33. [33]
  P. Gao, H. Wang, Y. Cheng, Chin. Chem. Lett. 33 (2022) 575–586. doi: 10.1016/j.cclet.2021.08.023
34. [34]
  L. Chibaya, B. Karim, H. Zhang, S. Jones, Proc. Natl. Acad. Sci. U. S. A. 118 (2021) e2003193118. doi: 10.1073/pnas.2003193118
35. [35]
  J.M. Replogle, W. Zhou, A.E. Amaro, et al., Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 30566. doi: 10.1073/pnas.2009506117
36. [36]
  J. Vitos-Faleato, S.M. Real, N. Gutierrez-Prat, et al., Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 2588. doi: 10.1073/pnas.1921404117
37. [37]
  J. Xie, L. Xia, W. Xiang, et al., Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 13012. doi: 10.1073/pnas.1918845117
1. [1]
  Xiangdong Lai , Tengfei Liu , Zengchao Guo , Yihan Wang , Jiang Xiao , Qingxiu Xia , Xiaohui Liu , Hui Jiang , Xuemei Wang . In situ formed fluorescent gold nanoclusters inhibit hair follicle regeneration in oxidative stress microenvironment via suppressing NFκB signal pathway. Chinese Chemical Letters, 2025, 36(2): 109762-. doi: 10.1016/j.cclet.2024.109762
2. [2]
  Wei GAO , Meiqi SONG , Xuan REN , Jianliang BAI , Jing SU , Jianlong MA , Zhijun WANG . A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1173-1182. doi: 10.11862/CJIC.20250112
3. [3]
  Jiayu Zeng , Minhui Liu , Ting Yang , Jia Huang , Songjiao Li , Wanting Zhang , Dan Cheng , Longwei He , Jia Zhou . Two-dimensional design strategy to construct smart dual-responsive fluorescent probe for the precise tracking of ischemic stroke. Chinese Chemical Letters, 2025, 36(5): 110166-. doi: 10.1016/j.cclet.2024.110166
4. [4]
  Chuanfeng Fan , Jian Gao , Yingkai Gao , Xintong Yang , Gaoning Li , Xiaochun Wang , Fei Li , Jin Zhou , Haifeng Yu , Yi Huang , Jin Chen , Yingying Shan , Li Chen . A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging. Chinese Chemical Letters, 2024, 35(10): 109838-. doi: 10.1016/j.cclet.2024.109838
5. [5]
  Chuyu Huang , Zhishan Liu , Linping Zhao , Zuxiao Chen , Rongrong Zheng , Xiaona Rao , Yuxuan Wei , Xin Chen , Shiying Li . Metal-coordinated oxidative stress amplifier to suppress tumor growth combined with M2 macrophage elimination. Chinese Chemical Letters, 2024, 35(12): 109696-. doi: 10.1016/j.cclet.2024.109696
6. [6]
  Huiyang Chen , Zibo Li , Xiaoying Li , Chenhong Tang , Xiaoyu Liu , Minyi Nie , Ying Huang , Xiaoyu Chen , Kuncai Liu , Yilan Dai , Qiaoling Zhang , Ling Lin , Siming Zhang , Bingchen Zhang , Zhiqiang Yu . A novel mitochondria-targeted nanoprodrug amplifies oxidative stress to enhance cisplatin chemotherapy for the treatment of hepatocellular carcinoma. Chinese Chemical Letters, 2025, 36(10): 111313-. doi: 10.1016/j.cclet.2025.111313
7. [7]
  Qian Pang , Fangjun Huo , Yongkang Yue , Caixia Yin . ONOO⁻ and viscosity dual-response fluorescent probe for arthritis imaging in vivo. Chinese Chemical Letters, 2025, 36(9): 110713-. doi: 10.1016/j.cclet.2024.110713
8. [8]
  Huan Ye , Ying Yang , Lirong Jiang , Taotao Zhe , Junchao Xu , Lintao Zeng . An intelligent sensing platform for discrimination of formaldehyde and nitrite in food. Chinese Chemical Letters, 2025, 36(11): 110840-. doi: 10.1016/j.cclet.2025.110840
9. [9]
  Lei Li , Guang Yang , Tianbai Xiong , Tingzhu Duan , Jia Wang , Xin Wang . Metal-free click polymerization of thiols and chalcone-derived internal olefins in air to prepare functional clusteroluminescent polythioethers for dual-response fluorescent probe. Chinese Chemical Letters, 2025, 36(11): 111374-. doi: 10.1016/j.cclet.2025.111374
10. [10]
  Liangji Chen , Zhen Yuan , Fudong Feng , Xin Zhou , Zhile Xiong , Wuji Wei , Hao Zhang , Banglin Chen , Shengchang Xiang , Zhangjing Zhang . A hydrogen-bonded organic framework containing fluorescent carbazole and responsive pyridyl units for sensing organic acids. Chinese Chemical Letters, 2024, 35(9): 109344-. doi: 10.1016/j.cclet.2023.109344
11. [11]
  Yang Liu , Leilei Zhang , Kaixuan Liu , Ling-Ling Wu , Hai-Yu Hu . Penicillin G acylase-responsive near-infrared fluorescent probe: Unravelling biofilm regulation and combating bacterial infections. Chinese Chemical Letters, 2024, 35(11): 109759-. doi: 10.1016/j.cclet.2024.109759
12. [12]
  Tian Cao , Xuyin Ding , Qiwen Peng , Min Zhang , Guoyue Shi . Intelligent laser-induced graphene sensor for multiplex probing catechol isomers. Chinese Chemical Letters, 2024, 35(7): 109238-. doi: 10.1016/j.cclet.2023.109238
13. [13]
  Sirui Chen , Ran Zhao , Li Dong , Qilin Liu , Wei Chen , Danna Liu , Maosheng Ye , Yingbo Li , Qiong Nie , Jingxin Meng , Shutao Wang . Smart antifouling coating integrating zwitterionic hydrogel with pH-responsive microcapsules for anti-crystal biofilm of orthodontic appliances. Chinese Chemical Letters, 2025, 36(12): 111647-. doi: 10.1016/j.cclet.2025.111647
14. [14]
  Weiqian Jin , Lin Liao , Tao Qin , Xiaoxuan Guan , Huyang Gao , Peng Liang , Ming Gao , Junyu Lu . Recent advances in nanomedicine therapy for bacterial pneumonia. Chinese Chemical Letters, 2025, 36(6): 110920-. doi: 10.1016/j.cclet.2025.110920
15. [15]
  Yuqing Liu , Shiling Zhang , Kai Jiang , Shiyue Ding , Limei Xu , Yingqi Liu , Ting Wang , Fenfen Zheng , Weiwei Xiong , Jun-Jie Zhu . Near-infrared light responsive upconversion-DNA nanocapsules for remote-controlled CRISPR-Cas9 genome editing. Chinese Chemical Letters, 2025, 36(5): 110282-. doi: 10.1016/j.cclet.2024.110282
16. [16]
  Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
17. [17]
  Xi Xue , Kai Chen , Hanyu Sun , Xiangying Liu , Xue Liu , Shize Li , Jingjie Yan , Yu Peng , Mohammad S. Mubarak , Ahmed Al-Harrasi , Hai-Yu Hu , Yafeng Deng , Xiandao Pan , Xiaojian Wang . Bidirectional Chemical Intelligent Net: A unified deep learning–based framework for predicting chemical reaction. Chinese Chemical Letters, 2025, 36(11): 110968-. doi: 10.1016/j.cclet.2025.110968
18. [18]
  Keyang Li , Yanan Wang , Yatao Xu , Guohua Shi , Sixian Wei , Xue Zhang , Baomei Zhang , Qiang Jia , Huanhua Xu , Liangmin Yu , Jun Wu , Zhiyu He . Flash nanocomplexation (FNC): A new microvolume mixing method for nanomedicine formulation. Chinese Chemical Letters, 2024, 35(10): 109511-. doi: 10.1016/j.cclet.2024.109511
19. [19]
  Zhongyu Wang , Lijun Wang , Huaixin Zhao . DNA-based nanosystems to generate reactive oxygen species for nanomedicine. Chinese Chemical Letters, 2024, 35(11): 109637-. doi: 10.1016/j.cclet.2024.109637
20. [20]
  Ruimiao Chang , Xinying Qu , Yuting Ye , Ying Qu , Bingyang Chu , Zhiyong Qian . Current advances in nanomedicine-based therapies for acute kidney injury. Chinese Chemical Letters, 2025, 36(10): 110802-. doi: 10.1016/j.cclet.2024.110802

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(1442)
HTML views(103)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142