Citation: Wen Zhang, Shaoqi Yang, Baozeng Ren, Xingmei Lu, Cai Jia. Study on ionic liquids based novel method for separation and purification of silkworm pupa protein[J]. Chinese Chemical Letters, ;2023, 34(1): 107474. doi: 10.1016/j.cclet.2022.04.072 shu

Study on ionic liquids based novel method for separation and purification of silkworm pupa protein

Wen Zhang ^{a, b, d} ,
Shaoqi Yang ^{a, c} ,
Baozeng Ren ^d ,
Xingmei Lu ^a ,
Cai Jia ^{a, *,}

a.
Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
b.
Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, China
c.
Key Laboratory of New Materials and Facilities for Rural Renewable Energy of Ministry of Agriculture and Rural Affairs, College of Mechanical & Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China
d.
School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China

cj@ipe.ac.cn

Received Date: 4 January 2022
Revised Date: 13 April 2022
Accepted Date: 25 April 2022
Available Online: 30 April 2022

Figures(10)

Silkworm pupa protein (SPP) that obtained by traditional method usually had a high fat content, which would impose restrictions on the further use of SPP. Herein, various functionalized ionic liquids (ILs) were used to extract SPP from silkworm pupae, the structure-performance relationship of ILs with their SPP separation performance were explored at the same time. The research showed that the maximum extraction yield of SPP was up to 62.6% with less than 0.5% low fat content by using 1-ethyl-3-methylimidazolium chloride ([Emim]Cl), when the dissolution experiment was conducted at 90 ℃ for 24 h with ethanol bath as the regeneration solvent. Comparing with the structure of raw material, the regenerated SPP maintained the native protein backbone. Meanwhile, all regenerated SPP showed a decreased crystallinity, which also exhibited decreased fraction of the α-helix comparing to that β-sheet united with coil random structures.
- Silkworm pupa protein,
- Ionic liquids,
- Protein backbone,
- Silkworm pupa,
- Separation
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