Citation: Peng Zhou, Jiawei Shen, Jun Liang, Tian Xue, Yuansheng Sun, Longhua Zhang, Changlin Tian. Metabolic state oscillations in cerebral nuclei detected using two-photon fluorescence lifetime imaging microscopy[J]. Chinese Chemical Letters, ;2023, 34(1): 107460. doi: 10.1016/j.cclet.2022.04.058 shu

Metabolic state oscillations in cerebral nuclei detected using two-photon fluorescence lifetime imaging microscopy

Peng Zhou ^a ,
Jiawei Shen ^b ,
Jun Liang ^b ,
Tian Xue ^b ,
Yuansheng Sun ^c ,
Longhua Zhang ^{b, *,} ,
Changlin Tian ^{a, b, *,}

a.
High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
b.
The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, and Center for BioAnalytical Chemistry, University of Science and Technology of China, Hefei 230026, China
c.
ISS, Inc., Champaign, IL 61822, United States

zlhustc@ustc.edu.cn

cltian@ustc.edu.cn

Received Date: 23 November 2021
Revised Date: 22 April 2022
Accepted Date: 24 April 2022
Available Online: 29 April 2022

Figures(4)

The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH), a key endogenous coenzyme and metabolic biomarker, can reflect the metabolic state of cells. To implement metabolic imaging of brain tissue at high resolution, we assembled a two-photon fluorescence lifetime imaging microscopy (FLIM) platform and verified the feasibility and stability of NADH-based two-photon FLIM in paraformaldehyde-fixed mouse cerebral slices. Furthermore, NADH based metabolic state oscillation was observed in cerebral nuclei suprachiasmatic nucleus (SCN). The free NADH fraction displayed a relatively lower level in the daytime than at the onset of night, and an ultradian oscillation at night was observed. Through the combination of high-resolution imaging and immunostaining data, the metabolic tendency of different cell types was detected after the first two hours of the day and at night. Thus, two-photon FLIM analysis of NADH in paraformaldehyde-fixed cerebral slices provides a high-resolution and label-free method to explore the metabolic state of deep brain regions.
- NADH,
- Fluorescence lifetime imaging microscopy,
- Brain metabolism,
- Metabolic oscillation,
- High resolution
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