Citation: Keda Chen, Xiaoxin Wu, Qingjing Wang, Ying Wang, Haiyan Zhang, Shuai Zhao, Chaonan Li, Zhiwen Hu, Zhimou Yang, Lanjuan Li. The protective effects of a d-tetra-peptide hydrogel adjuvant vaccine against H7N9 influenza virus in mice[J]. Chinese Chemical Letters, ;2023, 34(1): 107446. doi: 10.1016/j.cclet.2022.04.044 shu

The protective effects of a d-tetra-peptide hydrogel adjuvant vaccine against H7N9 influenza virus in mice

Keda Chen ^{a, b, 1} ,
Xiaoxin Wu ^{b, 1} ,
Qingjing Wang ^a ,
Ying Wang ^a ,
Haiyan Zhang ^c ,
Shuai Zhao ^b ,
Chaonan Li ^a ,
Zhiwen Hu ^{d, e} ,
Zhimou Yang ^{d, e, 1,} ,
Lanjuan Li ^{b, *,}

a.
Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China
b.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
c.
Zhejiang Shuren College, Zhejiang Chinese Medical University, Hangzhou 310053, China
d.
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Synergetic Innovation Center of Chemical Science and Engineering, and National Institute of Functional Materials, Nankai University, Tianjin 300071, China
e.
Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou 221004, China

yangzm@nankai.edu.cn

ljli@zju.edu.cn

Received Date: 23 December 2021
Revised Date: 9 April 2022
Accepted Date: 19 April 2022
Available Online: 22 April 2022

Figures(4)

Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans, with mortality reaching approximately 40%–50%. To prevent possible pandemics, the development of highly effective vaccines against influenza virus H7N9 is highly desired. In the present study, by taking advantage of the d-tetra-peptide adjuvant (G^DF^DF^DY), we reported a simple method to prepare H7N9 vaccines. Naproxen (Npx), with good anti inflammatory and broad anti-viral effects, was employed as an N-terminal capping group to construct a hydrogel precursor, Npx-G^DF^DF^DY. The hydrogel adjuvant was prepared using a routine heating cooling protocol and the final vaccine was ready after mixing with the split A/Zhejiang/DTID-ZJU01/2013 (H7N9) antigen by vortexing. Compared with the traditional Al(OH)₃ adjuvant vaccine and the split vaccine, our hydrogel adjuvant vaccine showed the best preventive effects against H7N9 infection. A mechanistic study illustrated that higher antibody responses and variations in cytokine expression might account for its increased protective effects. Our strategy demonstrated the advantages of a peptide hydrogel adjuvant in the application of vaccines against H7N9 and demonstrated its potential application in vaccines against emerging threats from other viruses.
- H7N9 influenza virus,
- Hydrogel,
- Adjuvant,
- Vaccine,
- Preventative effects
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