Citation: Jie Chen, Wenjuan Liu, Xiangning Fang, Qinglong Qiao, Zhaochao Xu. BODIPY 493 acts as a bright buffering fluorogenic probe for super-resolution imaging of lipid droplet dynamics[J]. Chinese Chemical Letters, ;2022, 33(12): 5042-5046. doi: 10.1016/j.cclet.2022.03.120 shu

BODIPY 493 acts as a bright buffering fluorogenic probe for super-resolution imaging of lipid droplet dynamics

Jie Chen ^{a, b} ,
Wenjuan Liu ^{a, b} ,
Xiangning Fang ^{a, b} ,
Qinglong Qiao ^a ,
Zhaochao Xu ^{a, *,}

a.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

zcxu@dicp.ac.cn

Received Date: 23 March 2022
Revised Date: 31 March 2022
Accepted Date: 31 March 2022
Available Online: 3 April 2022

Figures(4)

The need for temporal resolution and long-term stability in super-resolution fluorescence imaging has motivated research to improve the photostability of fluorescent probes. Due to the inevitable photobleaching of fluorophores, it is difficult to obtain long-term super-resolution imaging regardless of the self-healing strategy of introducing peroxide scavengers or the strategy of fluorophore structure modification to suppress TICT formation. The buffered fluorogenic probe uses the intact probes in the buffer pool to continuously replace the photobleached ones in the target, which greatly improves the photostability and enables stable dynamic super-resolution imaging for a long time. But the buffering capacity comes at the expense of reducing the number of fluorescent probes in targets, resulting in low staining fluorescence intensity. In this paper, we selected BODIPY 493, a lipid droplet probe with high fluorescence brightness, to explore the dynamic process of lipid droplet staining of this probe in cells. We found that BODIPY 493 only needs very low laser power for lipid droplet imaging due to the high molecular accumulation in lipid droplets and the high brightness, and the spatiotemporal resolution is greatly improved. More importantly, we found that BODIPY 493 also has a certain buffering capacity, which enables BODIPY 493 to be used for super-resolution imaging of lipid droplet dynamics. This work reminds researchers to coordinate the buffering capacity and brightness of fluorogenic probes.
- Lipid droplet,
- Super-resolution imaging,
- Fluorescent probe,
- Brightness,
- Buffering
1. [1]
  A.R. Thiam, R.V. Farese Jr, T.C. Walther, Nat. Rev. Mol. Cell Biol. 14 (2013) 775–786. doi: 10.1038/nrm3699
2. [2]
  M. Bosch, M. Sánchez-Álvarez, A. Fajardo, et al., Science 370 (2020) eaay8085. doi: 10.1126/science.aay8085
3. [3]
  M.A. Welte, A.P. Gould, Biochim. Biophys. Acta, Mol. Cell Biol. Lipids 1862 (2017) 1260–1272.
4. [4]
  R. Dubey, C.E. Stivala, H.Q. Nguyen, et al., Nat. Chem. Biol. 16 (2020) 206–213. doi: 10.1038/s41589-019-0447-7
5. [5]
  A. Romanauska, A. Köhler, Cell 174 (2018) 700–715. doi: 10.1016/j.cell.2018.05.047
6. [6]
  J.A. Olzmann, P. Carvalho, Nat. Rev. Mol. Cell Biol. 20 (2019) 137–155. doi: 10.1038/s41580-018-0085-z
7. [7]
  S. Martin, R.G. Parton, Nat. Rev. Mol. Cell Biol. 7 (2006) 373–378. doi: 10.1038/nrm1912
8. [8]
  F. Wilfling, J.T. Haas, T.C. Walther, et al., Curr. Opin. Cell Biol. 29 (2014) 39–45. doi: 10.1016/j.ceb.2014.03.008
9. [9]
  H. Tian, A.C. Sedgwick, H.H. Han, et al., Coord. Chem. Rev. 427 (2021) 213577. doi: 10.1016/j.ccr.2020.213577
10. [10]
  J. Zheng, S. Qin, L. Gui, et al., Chin. Chem. Lett. 32 (2021) 2385–2389. doi: 10.1016/j.cclet.2021.02.059
11. [11]
  Y.H. Pan, X.X. Chen, L. Dong, et al., Chin. Chem. Lett. 32 (2021) 3895–3898. doi: 10.1016/j.cclet.2021.06.024
12. [12]
  J. Chen, C. Wang, W. Liu, et al., Angew. Chem. Int. Ed. 60 (2021) 25104–25113. doi: 10.1002/anie.202111052
13. [13]
  R. Zhou, C. Wang, X. Liang, et al., ACS Mater. Lett. 3 (2021) 516–524. doi: 10.1021/acsmaterialslett.1c00143
14. [14]
  Q. Qiao, W. Liu, J. Chen, et al., Angew. Chem. Int. Ed. 60 (2021) 25104–25113.
15. [15]
  W. Zhou, X. Fang, Q. Qiao, et al., Chin. Chem. Lett. 32 (2021) 943–946. doi: 10.1016/j.cclet.2021.02.003
16. [16]
  W. Liu, Q. Qiao, J. Zheng, et al., Biosens. Bioelectron. 176 (2021) 112886. doi: 10.1016/j.bios.2020.112886
17. [17]
  X. Wu, D. Li, J. Li, et al., Chin. Chem. Lett. 32 (2021) 1937–1941. doi: 10.1016/j.cclet.2020.12.038
18. [18]
  C. Wang, W. Chi, Q. Qiao, et al., Chem. Soc. Rev. 50 (2021) 12656–12678. doi: 10.1039/d1cs00239b
19. [19]
  A. Loudet, K. Burgess, Chem. Rev. 107 (2007) 4891–4932. doi: 10.1021/cr078381n
20. [20]
  Y. Yan, X. Zhang, X. Zhang, et al., Chin. Chem. Lett. 31 (2020) 1091–1094. doi: 10.1016/j.cclet.2019.10.025
21. [21]
  Y. Zhao, W. Shi, X. Li, et al., Chem. Commun. 58 (2022) 1495–1509. doi: 10.1039/d1cc05717k
22. [22]
  B. Qiu, M.C. Simon, Bio-protocol. 6 (2016) e1912.
23. [23]
  X. Liu, Q. Qiao, W. Tian, et al., J. Am. Chem. Soc. 138 (2016) 6960–6963. doi: 10.1021/jacs.6b03924
24. [24]
  A. Romieu, C. Massif, S. Rihn, et al., New J. Chem. 37 (2013) 1016–1027. doi: 10.1039/c3nj41093e
25. [25]
  C.L. Jackson, Curr. Opin. Cell Biol. 59 (2019) 88–96. doi: 10.1016/j.ceb.2019.03.018
26. [26]
  M. Espe, S. Xie, S. Chen, et al., Aquacult. Nutr. 25 (2019) 737–746. doi: 10.1111/anu.12905
27. [27]
  T.K. Fam, A.S. Klymchenko, M. Collot, Materials(Basel) 11 (2018) 1768–1786. doi: 10.3390/ma11091768
28. [28]
  M.A. Welte, Biochem. Soc. Trans. 37 (2009) 991–996. doi: 10.1042/BST0370991
1. [1]
  Ying-Hao Pan , Xiao-Xiao Chen , Lei Dong , Na Shao , Li-Ya Niu , Qing-Zheng Yang . Visualizing nitric oxide-dependent HIF-1 activity under hypoxia with a lipid droplet-targeting fluorescent probe. Chinese Chemical Letters, 2021, 32(12): 3895-3898. doi: 10.1016/j.cclet.2021.06.024
2. [2]
  Qing Liu , Lin Xue , Dong-Jian Zhu , Guo-Ping Li , Hua Jiang . Highly selective two-photon fluorescent probe for imaging of nitric oxide in living cells. Chinese Chemical Letters, 2014, 25(1): 19-23.
3. [3]
  Hui Chen , Hui Wang , Xiao-Jun Qin , Chen Chen , Lu Feng , Lai-Zhong Chen , Lu-Pei Du , Min-Yong Li . A bestatin-based fl uorescent probe for aminopeptidase N cell imaging. Chinese Chemical Letters, 2015, 26(5): 513-516. doi: 10.1016/j.cclet.2015.01.023
4. [4]
  Hui-Ying Liu , Miao Zhao , Qing-Long Qiao , Hai-Jing Lang , Jing-Zhe Xu , Zhao-Chao Xu . Fluorescein-derived fl uorescent probe for cellular hydrogen sulfi de imaging. Chinese Chemical Letters, 2014, 25(7): 1060-1064. doi: 10.1016/j.cclet.2014.05.010
5. [5]
  Huimin Chen , Han Wang , Yongchun Wei , Maomao Hu , Bo Dong , Hongbao Fang , Qixin Chen . Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells. Chinese Chemical Letters, 2022, 33(4): 1865-1869. doi: 10.1016/j.cclet.2021.10.025
6. [6]
  Ming SUN , Di Hua SHANG GUAN , Hui Min MA , Li Hua NIE , Xiao Hua LIShao Xiang XIONG . A Novel Fluorescent Probe for Lead Ions. Chinese Chemical Letters, 2003, 14(10): 1024-1026.
7. [7]
  Xiao-Hang Yang , Shu Li , Zhi-Shu Tang , Xi-Di Yu , Tin Huang , Yong Gao . A simple, water-soluble, Fe³⁺-selective fluorescent probe and its application in bioimaging. Chinese Chemical Letters, 2015, 26(1): 129-132. doi: 10.1016/j.cclet.2014.09.025
8. [8]
  Tun Tang , Yu-Qi Chen , Bo-Shi Fu , Zhi-Yong He , Heng Xiao , Fan Wu , Jia-Qi Wang , Shao-Ru Wang , Xiang Zhou . A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells. Chinese Chemical Letters, 2016, 27(4): 540-544.
9. [9]
  Cheng Dan , Pan Yue , Yin Bin-Cheng , Yuan Lin , Zhang Xiao-Bing . A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress. Chinese Chemical Letters, 2017, 28(10): 1987-1990. doi: 10.1016/j.cclet.2017.08.021
10. [10]
  Feng Wei , Qiao Qing-Long , Leng Shuang , Miao Lu , Yin Wen-Ting , Wang Li-Qiu , Xu Zhao-Chao . A 1, 8-naphthalimide-derived turn-on fluorescent probe for imaging lysosomal nitric oxide in living cells. Chinese Chemical Letters, 2016, 27(9): 1554-1558. doi: 10.1016/j.cclet.2016.06.016
11. [11]
  Duan Houna , Ding Yu , Huang Chusen , Zhu Weiping , Wang Rui , Xu Yufang . A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells. Chinese Chemical Letters, 2019, 30(1): 55-57. doi: 10.1016/j.cclet.2018.03.016
12. [12]
  Zhang Baoxin , Zhang Haijuan , Zhong Miao , Wang Song , Xu Qianhe , Cho Dong-Hyung , Qiu Hongdeng . A novel off-on fluorescent probe for specific detection and imaging of cysteine in live cells and in vivo. Chinese Chemical Letters, 2020, 31(1): 133-135. doi: 10.1016/j.cclet.2019.05.061
13. [13]
  Shuangzhe Zhang , Haidong Li , Qichao Yao , Sahar Ghazali , Jiangli Fan , Jianjun Du , Jingyun Wang , Fengli Gao , Miao Li , Haibo Wang , Chengyong Dong , Xiaojun Peng . A unique two-photon fluorescent probe based on ICT mechanism for imaging palladium in living cells and mice. Chinese Chemical Letters, 2020, 31(11): 2913-2916. doi: 10.1016/j.cclet.2020.01.006
14. [14]
  Yuyu Wang , Houna Duan , Hongyuan Shi , Shiwei Zhang , Yufang Xu , Weiping Zhu , Xuhong Qian . A highly sensitive fluorescent probe for tracking intracellular zinc ions and direct imaging of prostatic tissue in mice. Chinese Chemical Letters, 2020, 31(11): 2933-2936. doi: 10.1016/j.cclet.2020.05.028
15. [15]
  Qing Wang , Jingwen Fan , Xiaoyan Bian , Hang Yao , Xiaohui Yuan , Ying Han , Chaoguo Yan . A microenvironment sensitive pillar[5]arene-based fluorescent probe for cell imaging and drug delivery. Chinese Chemical Letters, 2022, 33(4): 1979-1982. doi: 10.1016/j.cclet.2021.10.040
16. [16]
  Li Ling Hao , Jun Fen Li , Pei Hua Lin , Rui Feng Dong , Du Xin Li , Shao Min Shuang , Chuan Dong . A novel carbazole-based fluorescent probe: 3,6-Bis-[(N-ethylcarbazole-3-yl)-propene-1-keto]-N-ethylcarbazole. Chinese Chemical Letters, 2010, 21(1): 9-12. doi: 10.1016/j.cclet.2009.07.003
17. [17]
  Qin Huihuan , Li Lingling , Li Kun , Yu Xiaoqi . Novel strategy of constructing fluorescent probe for MAO-B via cascade reaction and its application in imaging MAO-B in human astrocyte. Chinese Chemical Letters, 2019, 30(1): 71-74. doi: 10.1016/j.cclet.2018.05.018
18. [18]
  Zheng Li , Xiaofeng Xia , Yu You , Cuifen Lu , Guichun Yang , Chao Ma , Junqi Nie , Qi Sun , Shuilin Wu , Jun Ren , Feiyi Wang . Fast-response fluorescent probe with favorable water solubility for highly sensitive imaging of endogenous tyrosinase in living cells and zebrafish model. Chinese Chemical Letters, 2021, 32(5): 1785-1789. doi: 10.1016/j.cclet.2020.12.053
19. [19]
  Xiwei Li , Caiyun Liu , Na Gao , Wenlong Sheng , Baocun Zhu . A melatonin-based targetable fluorescent probe for screening of tumor cells and real-time imaging of glutathione fluctuations in tumor cells. Chinese Chemical Letters, 2022, 33(5): 2527-2531. doi: 10.1016/j.cclet.2021.11.080
20. [20]
  Dong Ju , Sheng Mei Song , Yan Bo Wu , Shao Min Shuang , Chuan Dong . Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin. Chinese Chemical Letters, 2010, 21(4): 480-483. doi: 10.1016/j.cclet.2009.11.042

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