Citation: Jie Chen, Wenjuan Liu, Xiangning Fang, Qinglong Qiao, Zhaochao Xu. BODIPY 493 acts as a bright buffering fluorogenic probe for super-resolution imaging of lipid droplet dynamics[J]. Chinese Chemical Letters, ;2022, 33(12): 5042-5046. doi: 10.1016/j.cclet.2022.03.120 shu

BODIPY 493 acts as a bright buffering fluorogenic probe for super-resolution imaging of lipid droplet dynamics

Jie Chen ^{a, b} ,
Wenjuan Liu ^{a, b} ,
Xiangning Fang ^{a, b} ,
Qinglong Qiao ^a ,
Zhaochao Xu ^{a, *,}

a.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

zcxu@dicp.ac.cn

Received Date: 23 March 2022
Revised Date: 31 March 2022
Accepted Date: 31 March 2022
Available Online: 3 April 2022

Figures(4)

The need for temporal resolution and long-term stability in super-resolution fluorescence imaging has motivated research to improve the photostability of fluorescent probes. Due to the inevitable photobleaching of fluorophores, it is difficult to obtain long-term super-resolution imaging regardless of the self-healing strategy of introducing peroxide scavengers or the strategy of fluorophore structure modification to suppress TICT formation. The buffered fluorogenic probe uses the intact probes in the buffer pool to continuously replace the photobleached ones in the target, which greatly improves the photostability and enables stable dynamic super-resolution imaging for a long time. But the buffering capacity comes at the expense of reducing the number of fluorescent probes in targets, resulting in low staining fluorescence intensity. In this paper, we selected BODIPY 493, a lipid droplet probe with high fluorescence brightness, to explore the dynamic process of lipid droplet staining of this probe in cells. We found that BODIPY 493 only needs very low laser power for lipid droplet imaging due to the high molecular accumulation in lipid droplets and the high brightness, and the spatiotemporal resolution is greatly improved. More importantly, we found that BODIPY 493 also has a certain buffering capacity, which enables BODIPY 493 to be used for super-resolution imaging of lipid droplet dynamics. This work reminds researchers to coordinate the buffering capacity and brightness of fluorogenic probes.
- Lipid droplet,
- Super-resolution imaging,
- Fluorescent probe,
- Brightness,
- Buffering
1. [1]
  A.R. Thiam, R.V. Farese Jr, T.C. Walther, Nat. Rev. Mol. Cell Biol. 14 (2013) 775–786. doi: 10.1038/nrm3699
2. [2]
  M. Bosch, M. Sánchez-Álvarez, A. Fajardo, et al., Science 370 (2020) eaay8085. doi: 10.1126/science.aay8085
3. [3]
  M.A. Welte, A.P. Gould, Biochim. Biophys. Acta, Mol. Cell Biol. Lipids 1862 (2017) 1260–1272.
4. [4]
  R. Dubey, C.E. Stivala, H.Q. Nguyen, et al., Nat. Chem. Biol. 16 (2020) 206–213. doi: 10.1038/s41589-019-0447-7
5. [5]
  A. Romanauska, A. Köhler, Cell 174 (2018) 700–715. doi: 10.1016/j.cell.2018.05.047
6. [6]
  J.A. Olzmann, P. Carvalho, Nat. Rev. Mol. Cell Biol. 20 (2019) 137–155. doi: 10.1038/s41580-018-0085-z
7. [7]
  S. Martin, R.G. Parton, Nat. Rev. Mol. Cell Biol. 7 (2006) 373–378. doi: 10.1038/nrm1912
8. [8]
  F. Wilfling, J.T. Haas, T.C. Walther, et al., Curr. Opin. Cell Biol. 29 (2014) 39–45. doi: 10.1016/j.ceb.2014.03.008
9. [9]
  H. Tian, A.C. Sedgwick, H.H. Han, et al., Coord. Chem. Rev. 427 (2021) 213577. doi: 10.1016/j.ccr.2020.213577
10. [10]
  J. Zheng, S. Qin, L. Gui, et al., Chin. Chem. Lett. 32 (2021) 2385–2389. doi: 10.1016/j.cclet.2021.02.059
11. [11]
  Y.H. Pan, X.X. Chen, L. Dong, et al., Chin. Chem. Lett. 32 (2021) 3895–3898. doi: 10.1016/j.cclet.2021.06.024
12. [12]
  J. Chen, C. Wang, W. Liu, et al., Angew. Chem. Int. Ed. 60 (2021) 25104–25113. doi: 10.1002/anie.202111052
13. [13]
  R. Zhou, C. Wang, X. Liang, et al., ACS Mater. Lett. 3 (2021) 516–524. doi: 10.1021/acsmaterialslett.1c00143
14. [14]
  Q. Qiao, W. Liu, J. Chen, et al., Angew. Chem. Int. Ed. 60 (2021) 25104–25113.
15. [15]
  W. Zhou, X. Fang, Q. Qiao, et al., Chin. Chem. Lett. 32 (2021) 943–946. doi: 10.1016/j.cclet.2021.02.003
16. [16]
  W. Liu, Q. Qiao, J. Zheng, et al., Biosens. Bioelectron. 176 (2021) 112886. doi: 10.1016/j.bios.2020.112886
17. [17]
  X. Wu, D. Li, J. Li, et al., Chin. Chem. Lett. 32 (2021) 1937–1941. doi: 10.1016/j.cclet.2020.12.038
18. [18]
  C. Wang, W. Chi, Q. Qiao, et al., Chem. Soc. Rev. 50 (2021) 12656–12678. doi: 10.1039/d1cs00239b
19. [19]
  A. Loudet, K. Burgess, Chem. Rev. 107 (2007) 4891–4932. doi: 10.1021/cr078381n
20. [20]
  Y. Yan, X. Zhang, X. Zhang, et al., Chin. Chem. Lett. 31 (2020) 1091–1094. doi: 10.1016/j.cclet.2019.10.025
21. [21]
  Y. Zhao, W. Shi, X. Li, et al., Chem. Commun. 58 (2022) 1495–1509. doi: 10.1039/d1cc05717k
22. [22]
  B. Qiu, M.C. Simon, Bio-protocol. 6 (2016) e1912.
23. [23]
  X. Liu, Q. Qiao, W. Tian, et al., J. Am. Chem. Soc. 138 (2016) 6960–6963. doi: 10.1021/jacs.6b03924
24. [24]
  A. Romieu, C. Massif, S. Rihn, et al., New J. Chem. 37 (2013) 1016–1027. doi: 10.1039/c3nj41093e
25. [25]
  C.L. Jackson, Curr. Opin. Cell Biol. 59 (2019) 88–96. doi: 10.1016/j.ceb.2019.03.018
26. [26]
  M. Espe, S. Xie, S. Chen, et al., Aquacult. Nutr. 25 (2019) 737–746. doi: 10.1111/anu.12905
27. [27]
  T.K. Fam, A.S. Klymchenko, M. Collot, Materials(Basel) 11 (2018) 1768–1786. doi: 10.3390/ma11091768
28. [28]
  M.A. Welte, Biochem. Soc. Trans. 37 (2009) 991–996. doi: 10.1042/BST0370991
1. [1]
  Huamei Zhang , Jingjing Liu , Mingyue Li , Shida Ma , Xucong Zhou , Aixia Meng , Weina Han , Jin Zhou . Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe. Chinese Chemical Letters, 2024, 35(12): 110020-. doi: 10.1016/j.cclet.2024.110020
2. [2]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
3. [3]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
4. [4]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
5. [5]
  Tao Liu , Xuwei Han , Xueyi Sun , Weijie Zhang , Ke Gao , Runan Min , Yuting Tian , Caixia Yin . An activated fluorescent probe to monitor NO fluctuation in Parkinson’s disease. Chinese Chemical Letters, 2025, 36(3): 110170-. doi: 10.1016/j.cclet.2024.110170
6. [6]
  Fan Zheng , Runsha Xiao , Shuai Huang , Zhikang Chen , Chen Lai , Anyao Bi , Heying Yao , Xueping Feng , Zihua Chen , Wenbin Zeng . Accurate visualization colorectal cancer by monitoring viscosity variations with a novel mitochondria-targeted fluorescent probe. Chinese Chemical Letters, 2025, 36(2): 109876-. doi: 10.1016/j.cclet.2024.109876
7. [7]
  Zhixiao Xiong , Shanni Qiu , Yuyu Wang , Houna Duan , Yi Xiao , Yufang Xu , Weiping Zhu , Xuhong Qian . Photocalibrated NO release from the zinc ion fluorescent probe based on naphthalimide and its application in living cells. Chinese Chemical Letters, 2025, 36(4): 110002-. doi: 10.1016/j.cclet.2024.110002
8. [8]
  Chuanfeng Fan , Jian Gao , Yingkai Gao , Xintong Yang , Gaoning Li , Xiaochun Wang , Fei Li , Jin Zhou , Haifeng Yu , Yi Huang , Jin Chen , Yingying Shan , Li Chen . A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging. Chinese Chemical Letters, 2024, 35(10): 109838-. doi: 10.1016/j.cclet.2024.109838
9. [9]
  Lei Shen , Hongmei Liu , Ming Jin , Jinchao Zhang , Caixia Yin , Shuxiang Wang , Yutao Yang . “Three-in-one” strategy of trifluoromethyl regulated blood-brain barrier permeable fluorescent probe for peroxynitrite and antiepileptic evaluation of edaravone. Chinese Chemical Letters, 2024, 35(10): 109572-. doi: 10.1016/j.cclet.2024.109572
10. [10]
  Jiajia Lv , Jie Gao , Hongyu Li , Zeli Yuan , Nan Dong . Rational design of hydroxytricyanopyrrole-based probes with high affinity and rapid visualization for amyloid-β aggregates in vitro and in vivo. Chinese Chemical Letters, 2024, 35(5): 108940-. doi: 10.1016/j.cclet.2023.108940
11. [11]
  Chao Liu , Chao Jia , Shi-Xian Gan , Qiao-Yan Qi , Guo-Fang Jiang , Xin Zhao . A luminescent one-dimensional covalent organic framework for organic arsenic sensing in water. Chinese Chemical Letters, 2024, 35(11): 109750-. doi: 10.1016/j.cclet.2024.109750
12. [12]
  Yunkang Tong , Haiqiao Huang , Haolan Li , Mingle Li , Wen Sun , Jianjun Du , Jiangli Fan , Lei Wang , Bin Liu , Xiaoqiang Chen , Xiaojun Peng . Cooperative bond scission by HRP/H₂O₂ for targeted prodrug activation. Chinese Chemical Letters, 2024, 35(12): 109663-. doi: 10.1016/j.cclet.2024.109663
13. [13]
  Quan Zhang , Shunjie Xing , Jingqian Han , Li Feng , Jianchun Li , Zhaosheng Qian , Jin Zhou . Organic pollutant sensing for human health based on carbon dots. Chinese Chemical Letters, 2025, 36(1): 110117-. doi: 10.1016/j.cclet.2024.110117
14. [14]
  Hui Zhang , Rong Feng , Wanyi Yu , Hongbei Wei , Tianhong Wu , Peng Zhang , Wenhai Bian , Xin Li , Di Gao , Guojun Weng , Zhe Yang , Tony D. James , Xiaolong Sun . Evaluating the global thiols redox state in living cells using a reducing sulfur species responsive fluorescence switching platform. Chinese Chemical Letters, 2025, 36(4): 110528-. doi: 10.1016/j.cclet.2024.110528
15. [15]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
16. [16]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
17. [17]
  Fengkai Zou , Borui Su , Han Leng , Nini Xin , Shichao Jiang , Dan Wei , Mei Yang , Youhua Wang , Hongsong Fan . Red-emissive carbon quantum dots minimize phototoxicity for rapid and long-term lipid droplet monitoring. Chinese Chemical Letters, 2024, 35(10): 109523-. doi: 10.1016/j.cclet.2024.109523
18. [18]
  Rui Cheng , Tingting Zhang , Xin Huang , Jian Yu . Facile synthesis of high-brightness green-emitting carbon dots with narrow bandwidth towards backlight display. Chinese Chemical Letters, 2024, 35(5): 108763-. doi: 10.1016/j.cclet.2023.108763
19. [19]
  Linfang Wang , Jing Liu , Minghao Ren , Wei Guo . A highly sensitive fluorescent HClO probe for discrimination between cancerous and normal cells/tissues. Chinese Chemical Letters, 2024, 35(6): 108945-. doi: 10.1016/j.cclet.2023.108945
20. [20]
  Yang Liu , Leilei Zhang , Kaixuan Liu , Ling-Ling Wu , Hai-Yu Hu . Penicillin G acylase-responsive near-infrared fluorescent probe: Unravelling biofilm regulation and combating bacterial infections. Chinese Chemical Letters, 2024, 35(11): 109759-. doi: 10.1016/j.cclet.2024.109759

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(742)
HTML views(59)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142