Citation: Pengfei Zuo, Feng Gong, Yixia Yang, Xinghu Ji, Zhike He. In situ fluorescence imaging of fungi via (1, 3)-β-D-glucan aptamer and tyramide signal amplification technology[J]. Chinese Chemical Letters, ;2023, 34(1): 107259. doi: 10.1016/j.cclet.2022.02.064 shu

In situ fluorescence imaging of fungi via (1, 3)-β-D-glucan aptamer and tyramide signal amplification technology

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

xhu@whu.edu.cn

zhkhe@whu.edu.cn

Received Date: 10 November 2021
Revised Date: 6 February 2022
Accepted Date: 22 February 2022
Available Online: 25 February 2022

Figures(4)

Fungal infections are hazardous to human health that has drawn wide attention. In this work, a specific and sensitive method combing the recognition of aptamer to (1, 3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi. Fungi could be distinctly observed by fluorescence microscope rapidly. This method provides morphology and diagnostic information for identifying fungi. The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi, bacteria and even eukaryotic cell with the virtue of biomarkers.
- Fungi,
- (1, 3)-β-D-glucan,
- Aptamer,
- Tyramide signal amplification,
- Fluorescence imaging
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