Citation: Shengui He, Shufang Zhang, Xin Zhao, Xinyue Zhu, Lisen Chen, Jingnan Cui. Highly selective NIR fluorescent probe for acetylcholinesterase and its application in pesticide residues detection[J]. Chinese Chemical Letters, ;2022, 33(9): 4233-4237. doi: 10.1016/j.cclet.2022.02.020 shu

Highly selective NIR fluorescent probe for acetylcholinesterase and its application in pesticide residues detection

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

jncui@dlut.edu.cn

Received Date: 2 November 2021
Revised Date: 29 January 2022
Accepted Date: 9 February 2022
Available Online: 15 February 2022

Figures(9)

A series of near-infrared (NIR) fluorescent substrates (NDRO-1~8) derived from fluorophore NDRH with different volumes of ester bond as the recognition group were designed and synthesized for the detection of acetylcholinesterase (AChE), among which NDRO-1 with the smallest acetate group displayed the highest activity toward AChE. The detection limit of NDRO-1 for sensing AChE was 0.32 µg/mL, and K_m was 6.40 µmol/L, indicating ultra-sensitivity and good affinity of NDRO-1 toward AChE. NDRO-1 was used to detect the inhibitory of four kinds of pesticides including methamidophos, dichlorvos, and the detection limit was lower than 50 µg/L, which was further used in pesticide residues detection.
- NIR fluorescent probe,
- Acetylcholinesterase,
- Enzyme-activated,
- Pesticide residue detection
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