Citation: Lichun Wang, Yu Ding, Nan Li, Yamin Chai, Qiyu Li, Yunzheng Du, Zhangyong Hong, Lailiang Ou. Nanobody-based polyvinyl alcohol beads as antifouling adsorbents for selective removal of tumor necrosis factor-α[J]. Chinese Chemical Letters, ;2022, 33(5): 2512-2516. doi: 10.1016/j.cclet.2021.12.087 shu

Nanobody-based polyvinyl alcohol beads as antifouling adsorbents for selective removal of tumor necrosis factor-α

Lichun Wang ^a ,
Yu Ding ^b ,
Nan Li ^a ,
Yamin Chai ^a ,
Qiyu Li ^b ,
Yunzheng Du ^a ,
Zhangyong Hong ^{b, *,} ,
Lailiang Ou ^{a, *,}

a.
The key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
b.
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, College of Life Sciences, Nankai University, Tianjin 300071, China

hongzy@nankai.edu.cn

ouyll@nankai.edu.cn

Received Date: 12 July 2021
Revised Date: 27 December 2021
Accepted Date: 31 December 2021
Available Online: 7 January 2022

Figures(3)

Highly efficient removal of tumor necrosis factor-α (TNF-α) from plasma by hemoperfusion for autoimmune disease therapy remains a challenge in the clinical field owing to the low adsorption capacity and poor blood compatibility of adsorbents. In this work, a new class of nanobody (Nb)-coupled antifouling polyvinyl alcohol (PVA) beads was constructed as an immunosorbent for the selective removal of TNF-α from plasma. Notably, our immunosorbent exhibited an exceptionally high specific TNF-α adsorption capacity of 416.9 ng/g in human plasma (at a plasma-to-adsorbent ratio of 300). More importantly, the obtained adsorbent beads showed outstanding blood compatibility. In addition, during in vivo experiments, the blood circulation device was constructed to remove TNF-α in rat models, proving that the beads had good removal performance (~85%/60 min). Furthermore, 95% of the original capacity was retained after 6-month storage, showed strong stability and prolonged storage of PVA-Nb. Above all, the results indicate that the novel PVA-Nb immunosorbent has possible clinical applications for treating autoimmune diseases in the clinic.
- Nanobody,
- Polyvinyl alcohol beads,
- Immunosorbents,
- Tumor necrosis factor-α,
- Adsorption
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