Citation: Lichun Wang, Yu Ding, Nan Li, Yamin Chai, Qiyu Li, Yunzheng Du, Zhangyong Hong, Lailiang Ou. Nanobody-based polyvinyl alcohol beads as antifouling adsorbents for selective removal of tumor necrosis factor-α[J]. Chinese Chemical Letters, ;2022, 33(5): 2512-2516. doi: 10.1016/j.cclet.2021.12.087 shu

Nanobody-based polyvinyl alcohol beads as antifouling adsorbents for selective removal of tumor necrosis factor-α

Lichun Wang ^a ,
Yu Ding ^b ,
Nan Li ^a ,
Yamin Chai ^a ,
Qiyu Li ^b ,
Yunzheng Du ^a ,
Zhangyong Hong ^{b, *,} ,
Lailiang Ou ^{a, *,}

a.
The key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China
b.
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, College of Life Sciences, Nankai University, Tianjin 300071, China

hongzy@nankai.edu.cn

ouyll@nankai.edu.cn

Received Date: 12 July 2021
Revised Date: 27 December 2021
Accepted Date: 31 December 2021
Available Online: 7 January 2022

Figures(3)

Highly efficient removal of tumor necrosis factor-α (TNF-α) from plasma by hemoperfusion for autoimmune disease therapy remains a challenge in the clinical field owing to the low adsorption capacity and poor blood compatibility of adsorbents. In this work, a new class of nanobody (Nb)-coupled antifouling polyvinyl alcohol (PVA) beads was constructed as an immunosorbent for the selective removal of TNF-α from plasma. Notably, our immunosorbent exhibited an exceptionally high specific TNF-α adsorption capacity of 416.9 ng/g in human plasma (at a plasma-to-adsorbent ratio of 300). More importantly, the obtained adsorbent beads showed outstanding blood compatibility. In addition, during in vivo experiments, the blood circulation device was constructed to remove TNF-α in rat models, proving that the beads had good removal performance (~85%/60 min). Furthermore, 95% of the original capacity was retained after 6-month storage, showed strong stability and prolonged storage of PVA-Nb. Above all, the results indicate that the novel PVA-Nb immunosorbent has possible clinical applications for treating autoimmune diseases in the clinic.
- Nanobody,
- Polyvinyl alcohol beads,
- Immunosorbents,
- Tumor necrosis factor-α,
- Adsorption
1. [1]
  X.M. Zhou, Z.B. Zheng, H.Y. Liu, et al., Chin. Chem. Lett. 18 (2007) 905-908. doi: 10.1016/j.cclet.2007.06.014
2. [2]
  G. Cheng, Y. Chai, J. Chen, et al., Chem. Commun. 53 (2017) 7744-7747. doi: 10.1039/C7CC02479G
3. [3]
  H. Li, L. Liu, D. Zhang, et al., The Lancet 395 (2020) 1517-1520. doi: 10.1016/S0140-6736(20)30920-X
4. [4]
  C. Huang, Y. Wang, X. Li, et al., The Lancet 395 (2020) 497-506. doi: 10.1016/S0140-6736(20)30183-5
5. [5]
  B.B. Aggarwal, Nat. Rev. Immunol. 3 (2003) 745-756. doi: 10.1038/nri1184
6. [6]
  W. Sun, Y. Wu, M. Zheng, et al., J. Med. Chem. 63 (2020) 8146-8156. doi: 10.1021/acs.jmedchem.0c00377
7. [7]
  J.D. Lewis, New Engl. J. Med. 357 (2007) 296-298. doi: 10.1056/NEJMe078111
8. [8]
  T.T. Hansel, H. Kropshofer, T. Singer, et al., Nat. Rev. Drug Discov. 9 (2010) 325-338. doi: 10.1038/nrd3003
9. [9]
  J.H. Kang, M. Super, C.W. Yung, et al., Nat. Med. 20 (2014) 1211-1216. doi: 10.1038/nm.3640
10. [10]
  S. Shen, F. Han, A. Yuan, et al., Biomaterials 189 (2019) 60-68. doi: 10.1016/j.biomaterials.2018.10.029
11. [11]
  C. Shi, X. Wang, L. Wang, et al., Nat. Commun. 11 (2020) 1-13. doi: 10.1038/s41467-019-13993-7
12. [12]
  T. Eichhorn, S. Rauscher, C. Hammer, et al., Inflammation 39 (2016) 1737-1746. doi: 10.1007/s10753-016-0408-1
13. [13]
  C. Tripisciano, O.P. Kozynchenko, I. Linsberger, et al., Biomacromolecules 12 (2011) 3733-3740. doi: 10.1021/bm200982g
14. [14]
  S. Yachamaneni, G. Yushin, S.H. Yeon, et al., Biomaterials 31 (2010) 4789-4794. doi: 10.1016/j.biomaterials.2010.02.054
15. [15]
  C. Völlenkle, S. Weigert, N. Ilk, et al., Appl. Environ. Microbiol. 70 (2004) 1514-1521. doi: 10.1128/AEM.70.3.1514-1521.2004
16. [16]
  V. Weber, I. Linsberger, M. Ettenauer, et al., Biomacromolecules 6 (2005) 1864-1870. doi: 10.1021/bm040074t
17. [17]
  M.V. DiLeo, J.D. Fisher, B.M. Burton, et al., J. Biomed. Mater. Res. B: Appl. Biomater. 96B (2011) 127-133. doi: 10.1002/jbm.b.31748
18. [18]
  J. Chen, J. Sun, W. Han, et al., J. Mater. Chem. B 6 (2018) 4368-4379. doi: 10.1039/C8TB00563J
19. [19]
  A.M. Bossi, Nat. Chem. 12 (2020) 111-112. doi: 10.1038/s41557-019-0415-6
20. [20]
  T.W. Kang, I.J. Hwang, S. Lee, et al., Adv. Mater. 33 (2021) 2101376. doi: 10.1002/adma.202101376
21. [21]
  J. Xu, H. Miao, J. Wang, et al., Small 16 (2020) 1906644. doi: 10.1002/smll.201906644
22. [22]
  C. McMahon, A.S. Baier, R. Pascolutti, et al., Nat. Struct. Mol. Biol. 25 (2018) 289-296. doi: 10.1038/s41594-018-0028-6
23. [23]
  C. Hamers-Casterman, T. Atarhouch, S. Muyldermans, et al., Nature 363 (1993) 446-448. doi: 10.1038/363446a0
24. [24]
  R.M. Awad, Q. Lecocq, K. Zeven, et al., Mol. Ther. -Meth. Clin. D 22 (2021) 172-182. doi: 10.1016/j.omtm.2021.05.017
25. [25]
  C. Huang, J. Ren, F. Ji, et al., Acta Biomater. 107 (2020) 232-241. doi: 10.1016/j.actbio.2020.02.028
26. [26]
  Y. Liu, Y. Zhao, Y. Jia, et al., Chem. Eng. J. 424 (2021) 130303. doi: 10.1016/j.cej.2021.130303
27. [27]
  Z. Zhou, P. Zhao, C. Wang, et al., Microchim. Acta 187 (2020) 1-9. doi: 10.1007/s00604-019-3921-8
28. [28]
  S.M. Li, Y.S. Wang, S.T. Hsiao, et al., J. Mater. Chem. C 3 (2015) 9444-9453. doi: 10.1039/C5TC01564B
29. [29]
  J. Cohen, E. Abraham, J. Infect. Dis. 180 (1999) 116-121. doi: 10.1086/314839
30. [30]
  W. Zhu, X. Huang, Y. Zhang, et al., Chin. Chem. Lett. 32 (2021) 3382-3386. doi: 10.1016/j.cclet.2021.04.027
31. [31]
  Y.J. Wang, Y.T. Yu, Artif. Cell. Blood Sub. 39 (2011) 92-97. doi: 10.3109/10731199.2010.504924
32. [32]
  N.D. Paul, T. Krämer, J.E. McGrady, et al., Chem. Commun. 46 (2010) 7124-7126. doi: 10.1039/c0cc01880e
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  Viran P. Mahida , Manish P. Patel . Superabsorbent amphoteric nanohydrogels:Synthesis, characterization and dyes adsorption studies. Chinese Chemical Letters, 2016, 27(03): 471-474. doi: 10.1016/j.cclet.2015.12.015
2. [2]
  Yong Qiang He , Na Na Zhang , Xiao Dong Wang . Adsorption of graphene oxide/chitosan porous materials for metal ions. Chinese Chemical Letters, 2011, 22(7): 859-862. doi: 10.1016/j.cclet.2010.12.049
3. [3]
  Yun Tian . Characterization of nitrate ions adsorption and diffusion in P(DMAEMA/HEMA) hydrogels. Chinese Chemical Letters, 2008, 19(9): 1111-1114. doi: 10.1016/j.cclet.2008.05.040
4. [4]
  Xiu-Ming Liu , Dong-Qin He , Kuan-Jun Fang . Adsorption of cationic copolymer nanoparticles onto bamboo fiber surfaces measured by conductometric titration. Chinese Chemical Letters, 2015, 26(9): 1174-1178. doi: 10.1016/j.cclet.2015.05.006
5. [5]
  Jin Nan Wang , Ai Min Li Yang Zhou , Li Xu . Study on the influence of humic acid of different molecular weight on basic ion exchange resin's adsorption capacity. Chinese Chemical Letters, 2009, 20(12): 1478-1482. doi: 10.1016/j.cclet.2009.07.013
6. [6]
  Zhuo Guo , Li Feng Wang , Zhan Gao , Wei Wei Zhang . Equilibrium and kinetic studies on the adsorption of VB₁₂ onto CMK-3. Chinese Chemical Letters, 2007, 18(2): 233-236. doi: 10.1016/j.cclet.2006.12.032
7. [7]
  Chun Hai Yang , Cheng Guo Hu , Sheng Shui Hu . Predominating stable adsorption and direct electrochemistry of glucose oxidase on carbon nanotubes by oxygen-containing groups. Chinese Chemical Letters, 2007, 18(3): 313-315. doi: 10.1016/j.cclet.2007.01.021
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  Zu Pei LIANG , Ya Qing FENG , Zhi Yan LIANG , Shu Xian MENG . Kinetic of Adsorption of Urea Nitrogen onto Chitosan Coated Dialdehyde Cellulose under Catalysis of Immobilized Urease. Chinese Chemical Letters, 2005, 16(5): 697-700.
9. [9]
  Le-Chen Chen , Shan Lei , Mo-Zhen Wang , Jun Yang , Xue-Wu Ge . Fabrication of macroporous polystyrene/graphene oxide composite monolith and its adsorption property for tetracycline. Chinese Chemical Letters, 2016, 27(4): 511-517.
10. [10]
  Qian-Tao Shi , Wei Yan . Adsorption of arsenate on lanthanum-impregnated activated alumina: In situ ATR-FTIR and two-dimensional correlation analysis study. Chinese Chemical Letters, 2015, 26(2): 200-204. doi: 10.1016/j.cclet.2014.12.009
11. [11]
  Cheng Cheng , Jin Nan Wang , Li Xu , Ai Min Li . Preparation of new hyper cross-linked chelating resin for adsorption of Cu²⁺ and Ni²⁺ from water. Chinese Chemical Letters, 2012, 23(2): 245-248. doi: 10.1016/j.cclet.2011.10.019
12. [12]
  Hui Zhang , Fei Huang , De-Lei Liu , Peng Shi . Highly efficient removal of Cr(VI) from wastewater via adsorption with novel magnetic Fe₃O₄@C@MgAl-layered double-hydroxide. Chinese Chemical Letters, 2015, 26(9): 1137-1143. doi: 10.1016/j.cclet.2015.05.026
13. [13]
  Feng Ting Li , Hong Yang , Yan Zhao , Ran Xu . Novel modified pectin for heavy metal adsorption. Chinese Chemical Letters, 2007, 18(3): 325-328. doi: 10.1016/j.cclet.2007.01.034
14. [14]
  Gu Qing Xiao , Li Ping Long , Jiao Liang Wang . Synthesis of the water-compatible p-acetaminophen resin and its adsorption performances for vanillin in aqueous solution. Chinese Chemical Letters, 2012, 23(1): 123-126. doi: 10.1016/j.cclet.2011.10.007
15. [15]
  BAYOL E. , GVRTEN A. A. , DURSUN M. , KAYAKIRILMAZ K. . Adsorption Behavior and Inhibition Corrosion Effect of Sodium Carboxymethyl Cellulose on Mild Steel in Acidic Medium. Acta Physico-Chimica Sinica, 2008, 24(12): 2236-2242. doi: 10.1016/S1872-1508(08)60085-6
16. [16]
  SOLMAZ R. , MERT M. E. , KARDAS G. , YAZICI B. , ERBIL M. . Adsorption and Corrosion Inhibition Effect of 1,1’-Thiocarbonyldiimidazole on Mild Steel in H2SO4 Solution and Synergistic Effect of Iodide Ion. Acta Physico-Chimica Sinica, 2008, 24(07): 1185-1191. doi: 10.1016/S1872-1508(08)60053-4
17. [17]
  Meng-Qiao Wang , Qing Zhou , Man-Cheng Zhang , Chen-Dong Shuang , Yang Zhou , Ai-Min Li . Preparation of a novel magnetic resin for effective removal of both natural organic matter and organic micropollutants. Chinese Chemical Letters, 2013, 24(07): 601-604.
18. [18]
  Yue Sun , Zhi-Chao Li , Yan Xu . Preparation and application of a novel orotic acid chelating resin for removal of Cu(Ⅱ) in aqueous solutions. Chinese Chemical Letters, 2013, 24(8): 747-750.
19. [19]
  Marjaneh Samadizadeh , Ali Ahmadi Peyghan , Somayeh F. Rastegar . Sensing behavior of BN nanosheet toward nitrous oxide: A DFT study. Chinese Chemical Letters, 2015, 26(8): 1042-1045. doi: 10.1016/j.cclet.2015.05.048

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