Citation: Yixin Liu, Yuanyuan Yao, Beibei Yang, Yan-Jun Liu, Baohong Liu. In-situ monitoring of cell-secreted lactate by electrochemiluminescence sensing under biomimetic microfluidic confinement[J]. Chinese Chemical Letters, ;2022, 33(5): 2705-2707. doi: 10.1016/j.cclet.2021.09.074 shu

In-situ monitoring of cell-secreted lactate by electrochemiluminescence sensing under biomimetic microfluidic confinement

Yixin Liu ^a ,
Yuanyuan Yao ^c ,
Beibei Yang ^d ,
Yan-Jun Liu ^{b, *,} ,
Baohong Liu ^{a, *,}

a.
Department of Chemistry, Shanghai Stomatological Hospital, and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China
b.
Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
c.
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China
d.
Department of Chemistry and Chemical Engineering, Nantong University, Nantong 226000, China

yanjun_liu@fudan.edu.cn

bhliu@fudan.edu.cn

Received Date: 31 May 2021
Revised Date: 6 September 2021
Accepted Date: 22 September 2021
Available Online: 29 September 2021

Figures(4)

Cell migration proceeds in 3D matrices in vivo, which can naturally switch to distinct phenotypes for better invasion in confined microenvironments. The studies of important metabolites under confinement are extremely meaningful for comprehensive insights into cancer metastasis. The integration of cell confinement device and analytical techniques is a key point for in-situ analysis of significant metabolites in vitro. Herein, an electrochemiluminescence (ECL) sensing platform was designed for in-situ monitoring of cell-secreted lactate in highly confined microenvironments. The 3-µm confiner was exactly fabricated via microfabrication and microfluidics technique, and cells in high confinement and low adhesion tended to be round with contractile blebs on cell margins. Significantly, in-situ monitoring of lactate was successfully achieved on the ECL platform with the catalysis of lactate oxidase, in which the levels in different time intervals were acquired in the luminol-hydrogen peroxide system. Furthermore, the results were verified by the liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology, which showed similar fluctuations with the ECL platform. This system offered an available avenue for metabolites analysis in highly confined microenvironments, which may advance deeper insights into metabolic mechanisms of cancer metastasis
- Confinement,
- ECL sensing platform,
- In-situ monitoring
1. [1]
  N. Reymond, B.B. d'Água, A.J. Ridley, Nat. Rev. Cancer13 (2013) 858–870. doi: 10.1038/nrc3628
2. [2]
  J.A. Joyce, J.W. Pollard, Nat. Rev. Cancer9 (2009) 239–252. doi: 10.1038/nrc2618
3. [3]
  P. Friedl, Curr. Opin. Cell Biol. 16 (2004) 14–23. doi: 10.1016/j.ceb.2003.11.001
4. [4]
  H.M. Estabridis, A. Jana, A. Nain, D.J. Odde, Ann. Biomed. Eng. 46 (2018) 392–403. doi: 10.1007/s10439-017-1958-6
5. [5]
  K. Wolf, S. Alexander, V. Schacht, et al., Semin. Cell Dev. Biol20 (2009) 931–941. doi: 10.1016/j.semcdb.2009.08.005
6. [6]
  J.W. Hou, X. Liu, S.B. Zhou, View 2 (2021) 20200023. doi: 10.1002/viw.20200023
7. [7]
  L. Miccio, F. Cimmino, I. Kurelac, et al., View1 (2020) 20200034. doi: 10.1002/viw.20200034
8. [8]
  R. Zhang, B. Le, W. Xu, et al., Small Methods3 (2019) 1800474. doi: 10.1002/smtd.201800474
9. [9]
  H.T. Tan, H.Q. Hu, L. Huang, K. Qian, Analyst145 (2020) 5699–5712. doi: 10.1039/d0an00577k
10. [10]
  J.G. Liu, C.L. Cai, Y.N. Wang, et al., Adv. Sci. 7 (2020) 1903730–1903739. doi: 10.1002/advs.201903730
11. [11]
  S.Y. Sun, R.M. Wang, Y.D. Huang, et al., Small15 (2019) 1902441–1902449. doi: 10.1002/smll.201902441
12. [12]
  P. Friedl, S. Alexander, Cell147 (2011) 992–1009. doi: 10.1016/j.cell.2011.11.016
13. [13]
  S. Alexander, B. Weigelin, F. Winkler, P. Friedl, Curr. Opin. Cell Biol. 25 (2013) 659–671. doi: 10.1016/j.ceb.2013.07.001
14. [14]
  Y.J. Liu, M.L. Berre, F. Lautenschlaeger, et al., Cell160 (2015) 659–672. doi: 10.1016/j.cell.2015.01.007
15. [15]
  G. Bergers, S.M. Fendt, Nat. Rev. Cancer21 (2021) 162–180. doi: 10.1038/s41568-020-00320-2
16. [16]
  A.L. Hu, Y.H. Liu, H.H. Deng, et al., Biosens. Bioelectron. 61 (2014) 374–378. doi: 10.1016/j.bios.2014.05.048
17. [17]
  N. Nesakumar, K. Thandavan, S. Sethuraman, et al., J. Colloid Interface Sci. 414 (2014) 90–96. doi: 10.1016/j.jcis.2013.09.052
18. [18]
  K.K. Hussain, N.G. Gurudatt, M.H. Akthar, et al., Biosens. Bioelectron. 155 (2020) 112094–112102. doi: 10.1016/j.bios.2020.112094
19. [19]
  S. Majeed, W.Y. Gao, Y. Zholudov, K. Muzyka, G.B. Xu, Electroanalysis28 (2016) 2672–2679. doi: 10.1002/elan.201600209
20. [20]
  T.T. Zhao, C.S. Lin, Q.H. Yao, X. Chen, Talanta154 (2016) 492–497. doi: 10.1016/j.talanta.2016.03.091
21. [21]
  X. Xiong, Y. Zhang, Y.F. Wang, et al., Sens. Actuators B297 (2019) 126812–126820. doi: 10.1016/j.snb.2019.126812
22. [22]
  Y. Zou, H.X. Zhang, Z.H. Wang, et al., Talanta198 (2019) 39–44. doi: 10.5539/ells.v9n3p39
23. [23]
  Y.J. Liu, M.L. Berre, F. Lautenschlaeger, et al., Cell160 (2015) 659–672. doi: 10.1016/j.cell.2015.01.007
24. [24]
  E. Sahai, C.J. Marshall, Nat. Cell Biol. 5 (2003) 711–719. doi: 10.1038/ncb1019
1. [1]
  Xiao Yan , Xiao-Zheng Lan . Phase behavior of undecane-tetradecane mixtures confined in SBA-15. Chinese Chemical Letters, 2013, 24(10): 885-888.
2. [2]
  Xiao Zheng Lan , Hai Rong Pei , Chuan Xian Cheng . Phase behavior of binary system of bromobenzene-chlorobenzene confined in SBA-15 and MCM-41. Chinese Chemical Letters, 2011, 22(12): 1497-1500. doi: 10.1016/j.cclet.2011.07.007
3. [3]
  Zheng Tangfei , Jiang Jinxia , Wang Jian , Hu Sufang , Ding Wei , Wei Zidong . Regulation of Electrocatalysts Based on Confinement-Induced Properties. Acta Physico-Chimica Sinica, 2021, 37(11): 2011027-0. doi: 10.3866/PKU.WHXB202011027
4. [4]
  Guorui Wu , Wenshuai Li , Weijun Du , Aiqin Yue , Jinzhong Zhao , Dingbin Liu . In-situ monitoring of nitrile-bearing pesticide residues by background-free surface-enhanced Raman spectroscopy. Chinese Chemical Letters, 2022, 33(1): 519-522. doi: 10.1016/j.cclet.2021.06.051
5. [5]
  Meng Dan , Si Jianpeng , Wang Mingyue , Wang Guosheng , Shen Yanbai , San Xiaoguang , Meng Fanli . In-situ growth of V₂O₅ flower-like structures on ceramic tubes and their trimethylamine sensing properties. Chinese Chemical Letters, 2020, 31(8): 2133-2136. doi: 10.1016/j.cclet.2019.12.021
6. [6]
  Li Tingting , Shen Yanbai , Zhao Sikai , Zhou Pengfei , Zhong Xiangxi , Gao Shuling , Wei Dezhou , Meng Fanli . Synthesis and in-situ noble metal modification of WO₃·0.33H₂O nanorods from a tungsten-containing mineral for enhancing NH₃ sensing performance. Chinese Chemical Letters, 2020, 31(8): 2037-2040. doi: 10.1016/j.cclet.2020.01.024
7. [7]
  Jian Min ZHANG , Qiu Zhi SHI , Chang Chun YANG , Quan Feng DONG . In-situ pH Measurement at the Electrode/Solution Interface. Chinese Chemical Letters, 2000, 11(7): 617-620.
8. [8]
  Lingbo Zong , Xin Chen , Shuming Dou , Kaicai Fan , Zumin Wang , Wenjun Zhang , Yunmei Du , Jie Xu , Xiaofei Jia , Qi Zhang , Xia Li , Yida Deng , Yanan Chen , Lei Wang . Stable confinement of Fe/Fe₃C in Fe, N-codoped carbon nanotube towards robust zinc-air batteries. Chinese Chemical Letters, 2021, 32(3): 1121-1126. doi: 10.1016/j.cclet.2020.08.029
9. [9]
  M. Rashid , Shams Qamar Usmani , Suhail Sabir . In-situ copolymerization of aniline with alkyl amine by APS:Kinetics and application. Chinese Chemical Letters, 2008, 19(11): 1358-1362. doi: 10.1016/j.cclet.2008.06.045
10. [10]
  Lan Dong LI , Ji Xin CHEN , Shu Juan ZHANG , Nai Jia GUAN . In-situ Synthesis of Binderless ZSM-5 Zeolitic Coatings on Aluminum. Chinese Chemical Letters, 2005, 16(2): 253-256.
11. [11]
  Zi Pin ZHANG , Bao Kang JIN , Yu Peng TIAN , Shi Jun WANG . Electrochemistry and In-situ Spectroelectrochemistry Studies of Carbazole and 9-Ethylcarbazole. Chinese Chemical Letters, 2005, 16(12): 1621-1624.
12. [12]
  Lin Jie ZHI , Jin Yue TANG , Tong ZHAO , Yun Zhao YU . Preparation of Phenolic Resin/Silver Nanocomposites via in-situ Reduction. Chinese Chemical Letters, 2003, 14(4): 425-428.
13. [13]
  Peng DU , Bao Kang JIN , Jia Xiang YANG , Xiang Qin LIN . In-situ FTIR Spectroelectrochemical and Electrochemical Studies of Ferrocene and Derivatives at a Platinum Electrode. Chinese Chemical Letters, 2004, 15(2): 204-207.
14. [14]
  Ai Li BO , Xiang Qin LIN . In-situ FTIR Spectroelectrochemical Study of Chromium Hexacyanoferrate on Glassy Carbon Electrode. Chinese Chemical Letters, 1998, 9(9): 851-854.
15. [15]
  Feng Sheng , Yan Pengcheng , Xu Li , Xia Jiexiang , Li Huaming . Exploitation of a photoelectrochemical sensing platform for bisphenol A quantitative determination using Cu/graphitic carbon nitride nanocomposites. Chinese Chemical Letters, 2018, 29(11): 1629-1632. doi: 10.1016/j.cclet.2018.08.010
16. [16]
  Lai Yujian , Dong Lijie , Liu Rui , Lu Shaoyu , He Zuoliang , Shan Wanyu , Geng Fanglan , Cai Yaqi , Liu Jingfu . Synthesis of highly-branched Au@AgPd core/shell nanoflowers for in situ SERS monitoring of catalytic reactions. Chinese Chemical Letters, 2020, 31(9): 2437-2441. doi: 10.1016/j.cclet.2020.04.050
17. [17]
  Danni Guo , Lixia Zhao , Hui Zhang . Crystallinity engineering of Au nanoparticles on graphene for in situ SERS monitoring of Fenton-like reaction. Chinese Chemical Letters, 2022, 33(3): 1263-1266. doi: 10.1016/j.cclet.2021.07.051
18. [18]
  LUO Ya , FU Lu-Lu , XIANG Jing . Three New Zn(Ⅱ) Coordination Compounds Based on the in-situ Formed Tetrazolate Ligand: Syntheses, Crystal Structures and Luminescence Properties. Chinese Journal of Structural Chemistry, 2015, 34(1): 95-102. doi: 10.14102/j.cnki.0254-5861.2011-404
19. [19]
  Shu Ping XU , Huan Wen CHEN , Ai Min YU , Qin Han JIN , Hua Ying LIAO . Rapid In-situ Determination of Chromium (VI) in Water Using Modified Diphenylcarbazine with Citrate. Chinese Chemical Letters, 2002, 13(7): 660-661.
20. [20]
  Xue Lei SHAN , Nai Jia GUAN , Xiang ZENG , Shou He XIANG . In-situ Synthesis of ZSM-5 with Different Si/AlRatios on Honeycombshaped Cordierite and their Behavior on NO Decomposition. Chinese Chemical Letters, 1999, 10(10): 885-888.

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(28)
HTML views(2)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142